CoChat
Sign Up
Cited by 58

The DOI matches its Crossref record.

A Fundamental Role of Myh9 for Neutrophil Migration in Innate Immunity

Zehrer, Annette, Pick, Robert, Salvermoser, Melanie, Boda, Annegret, Miller, Meike, Stark, Konstantin, Weckbach, Ludwig T, Walzog, Barbara, Begandt, Daniela·2018·The Journal of Immunology
DOI 10.4049/jimmunol.1701400
Open this paper in CoChat — free account

Abstract

Neutrophils are the first leukocytes to arrive at sites of injury during the acute inflammatory response. To maintain the polarized morphology during migration, nonmuscle myosins class II are essential, but studies using genetic models to investigate the role of Myh9 for neutrophil migration were missing. In this study, we analyzed the functional role of Myh9 on neutrophil trafficking using genetic downregulation of Myh9 in Vav-iCre+/Myh9wt/fl mice because the complete knockout of Myh9 in the hematopoietic system was lethal. Migration velocity and Euclidean distance were significantly diminished during mechanotactic migration of Vav-iCre+/Myh9wt/fl neutrophils compared with Vav-iCre−/Myh9wt/fl control neutrophils. Similar results were obtained for transmigration and migration in confined three-dimensional environments. Stimulated emission depletion nanoscopy revealed that a certain threshold of Myh9 was required to maintain proper F-actin dynamics in the front of the migrating cell. In laser-induced skin injury and in acute peritonitis, reduced Myh9 expression in the hematopoietic system resulted in significantly diminished neutrophil extravasation. Investigation of bone marrow chimeric mice in the peritonitis model revealed that the migration defect was cell intrinsic. Expression of Myh9-EGFP rescued the Myh9-related defects in two-dimensional and three-dimensional migration of Hoxb8-SCF cell–derived neutrophils generated from fetal liver cells with a Myh9 knockdown. Live cell imaging provided evidence that Myh9 was localized in branching lamellipodia and in the uropod where it may enable fast neutrophil migration. In summary, the severe migration defects indicate an essential and fundamental role of Myh9 for neutrophil trafficking in innate immunity.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.4049/jimmunol.1701400

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 52

  1. Ley, 2007, Getting to the site of inflammation: the leukocyte adhesion cascade updated, Nat. Rev. Immunol., 7, 678, 10.1038/nri2156 · DOI · Paper page
  2. Nourshargh, 2014, Leukocyte migration into inflamed tissues, Immunity, 41, 694, 10.1016/j.immuni.2014.10.008 · DOI
  3. Mócsai, 2015, Intracellular signalling during neutrophil recruitment, Cardiovasc. Res., 107, 373, 10.1093/cvr/cvv159 · DOI
  4. Begandt, 2017, How neutrophils resist shear stress at blood vessel walls: molecular mechanisms, subcellular structures, and cell-cell interactions, J. Leukoc. Biol., 102, 699, 10.1189/jlb.3MR0117-026RR · DOI
  5. Lämmermann, 2008, Rapid leukocyte migration by integrin-independent flowing and squeezing, Nature, 453, 51, 10.1038/nature06887 · DOI · Paper page
  6. Renkawitz, 2009, Adaptive force transmission in amoeboid cell migration, Nat. Cell Biol., 11, 1438, 10.1038/ncb1992 · DOI
  7. Pestonjamasp, 2006, Rac1 links leading edge and uropod events through Rho and myosin activation during chemotaxis, Blood, 108, 2814, 10.1182/blood-2006-01-010363 · DOI
  8. Xu, 2003, Divergent signals and cytoskeletal assemblies regulate self-organizing polarity in neutrophils, Cell, 114, 201, 10.1016/S0092-8674(03)00555-5 · DOI
  9. Liu, 2015, Moesin and myosin phosphatase confine neutrophil orientation in a chemotactic gradient, J. Exp. Med., 212, 267, 10.1084/jem.20140508 · DOI
  10. Golomb, 2004, Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family, J. Biol. Chem., 279, 2800, 10.1074/jbc.M309981200 · DOI
  11. Vicente-Manzanares, 2009, Non-muscle myosin II takes centre stage in cell adhesion and migration, Nat. Rev. Mol. Cell Biol., 10, 778, 10.1038/nrm2786 · DOI
  12. Maupin, 1994, Differential localization of myosin-II isozymes in human cultured cells and blood cells, J. Cell Sci., 107, 3077, 10.1242/jcs.107.11.3077 · DOI
  13. Marigo, 2004, Correlation between the clinical phenotype of MYH9-related disease and tissue distribution of class II nonmuscle myosin heavy chains, Genomics, 83, 1125, 10.1016/j.ygeno.2003.12.012 · DOI · Paper page
  14. Shin, 2014, Contractile forces sustain and polarize hematopoiesis from stem and progenitor cells, Cell Stem Cell, 14, 81, 10.1016/j.stem.2013.10.009 · DOI
  15. Képiró, 2014, para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor, Angew. Chem. Int. Ed. Engl., 53, 8211, 10.1002/anie.201403540 · DOI
  16. Kolega, 2004, Phototoxicity and photoinactivation of blebbistatin in UV and visible light, Biochem. Biophys. Res. Commun., 320, 1020, 10.1016/j.bbrc.2004.06.045 · DOI
  17. Ostap, 2002, 2,3-Butanedione monoxime (BDM) as a myosin inhibitor, J. Muscle Res. Cell Motil., 23, 305, 10.1023/A:1022047102064 · DOI
  18. Houk, 2012, Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration, Cell, 148, 175, 10.1016/j.cell.2011.10.050 · DOI
  19. Stroka, 2013, Human neutrophil cytoskeletal dynamics and contractility actively contribute to trans-endothelial migration, PLoS One, 8, e61377, 10.1371/journal.pone.0061377 · DOI
  20. Eddy, 2000, Ca2+-dependent myosin II activation is required for uropod retraction during neutrophil migration, J. Cell Sci., 113, 1287, 10.1242/jcs.113.7.1287 · DOI
  21. Jacobelli, 2013, Activated T cell trans-endothelial migration relies on myosin-IIA contractility for squeezing the cell nucleus through endothelial cell barriers, PLoS One, 8, e75151, 10.1371/journal.pone.0075151 · DOI
  22. Jacobelli, 2010, Confinement-optimized three-dimensional T cell amoeboid motility is modulated via myosin IIA-regulated adhesions, Nat. Immunol., 11, 953, 10.1038/ni.1936 · DOI
  23. Jacobelli, 2009, Myosin-IIA and ICAM-1 regulate the interchange between two distinct modes of T cell migration, J. Immunol., 182, 2041, 10.4049/jimmunol.0803267 · DOI
  24. Chabaud, 2015, Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. [Published erratum appears in 2015 Nat. Commun. 6: 8122.], Nat. Commun., 6, 7526, 10.1038/ncomms8526 · DOI
  25. Léon, 2007, Megakaryocyte-restricted MYH9 inactivation dramatically affects hemostasis while preserving platelet aggregation and secretion, Blood, 110, 3183, 10.1182/blood-2007-03-080184 · DOI
  26. de Boer, 2003, Transgenic mice with hematopoietic and lymphoid specific expression of Cre, Eur. J. Immunol., 33, 314, 10.1002/immu.200310005 · DOI · Paper page
  27. Schymeinsky, 2006, The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration, Blood, 108, 3919, 10.1182/blood-2005-12-030387 · DOI
  28. Schymeinsky, 2007, Syk-mediated translocation of PI3Kdelta to the leading edge controls lamellipodium formation and migration of leukocytes, PLoS One, 2, e1132, 10.1371/journal.pone.0001132 · DOI
  29. Wang, 2006, Quantitative production of macrophages or neutrophils ex vivo using conditional Hoxb8, Nat. Methods, 3, 287, 10.1038/nmeth865 · DOI
  30. Redecke, 2013, Hematopoietic progenitor cell lines with myeloid and lymphoid potential, Nat. Methods, 10, 795, 10.1038/nmeth.2510 · DOI
  31. Southgate, 2008, Identification of formyl peptides from Listeria monocytogenes and Staphylococcus aureus as potent chemoattractants for mouse neutrophils, J. Immunol., 181, 1429, 10.4049/jimmunol.181.2.1429 · DOI
  32. Yan, 1997, Signaling by adhesion in human neutrophils: activation of the p72syk tyrosine kinase and formation of protein complexes containing p72syk and Src family kinases in neutrophils spreading over fibrinogen, J. Immunol., 158, 1902, 10.4049/jimmunol.158.4.1902 · DOI
  33. Shivanandan, 2013, MosaicIA: an ImageJ/Fiji plugin for spatial pattern and interaction analysis, BMC Bioinformatics, 14, 349, 10.1186/1471-2105-14-349 · DOI
  34. Helmuth, 2010, Beyond co-localization: inferring spatial interactions between sub-cellular structures from microscopy images, BMC Bioinformatics, 11, 372, 10.1186/1471-2105-11-372 · DOI
  35. Salvermoser, 2018, Myosin 1f is specifically required for neutrophil migration in 3D environments during acute inflammation, Blood, 131, 1887, 10.1182/blood-2017-10-811851 · DOI
  36. Schymeinsky, 2009, A fundamental role of mAbp1 in neutrophils: impact on beta(2) integrin-mediated phagocytosis and adhesion in vivo, Blood, 114, 4209, 10.1182/blood-2009-02-206169 · DOI
  37. Pick, 2017, Coronin 1A, a novel player in integrin biology, controls neutrophil trafficking in innate immunity, Blood, 130, 847, 10.1182/blood-2016-11-749622 · DOI
  38. Zigmond, 1977, Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors, J. Cell Biol., 75, 606, 10.1083/jcb.75.2.606 · DOI
  39. Hepper, 2012, The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions, J. Immunol., 188, 4590, 10.4049/jimmunol.1100878 · DOI
  40. Kurz, 2016, MST1-dependent vesicle trafficking regulates neutrophil transmigration through the vascular basement membrane, J. Clin. Invest., 126, 4125, 10.1172/JCI87043 · DOI
  41. Ng, 2008, Migratory dermal dendritic cells act as rapid sensors of protozoan parasites, PLoS Pathog., 4, e1000222, 10.1371/journal.ppat.1000222 · DOI
  42. Stark, 2013, Capillary and arteriolar pericytes attract innate leukocytes exiting through venules and ‘instruct’ them with pattern-recognition and motility programs, Nat. Immunol., 14, 41, 10.1038/ni.2477 · DOI · Paper page
  43. Khandoga, 2009, In vivo imaging and quantitative analysis of leukocyte directional migration and polarization in inflamed tissue, PLoS One, 4, e4693, 10.1371/journal.pone.0004693 · DOI
  44. Conti, 2004, Defects in cell adhesion and the visceral endoderm following ablation of nonmuscle myosin heavy chain II-A in mice, J. Biol. Chem., 279, 41263, 10.1074/jbc.C400352200 · DOI
  45. Shin, 2010, Spatiotemporal organization, regulation, and functions of tractions during neutrophil chemotaxis, Blood, 116, 3297, 10.1182/blood-2009-12-260851 · DOI
  46. Mortensen, 2010, Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo, Proc. Natl. Acad. Sci. USA, 107, 832, 10.1073/pnas.0913170107 · DOI
  47. Finney, 2012, CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development, Blood, 119, 1747, 10.1182/blood-2011-09-380709 · DOI
  48. Pecci, 2005, Pathogenetic mechanisms of hematological abnormalities of patients with MYH9 mutations, Hum. Mol. Genet., 14, 3169, 10.1093/hmg/ddi344 · DOI
  49. Klapproth, 2015, Minimal amounts of kindlin-3 suffice for basal platelet and leukocyte functions in mice, Blood, 126, 2592, 10.1182/blood-2015-04-639310 · DOI
  50. Ding, 1999, Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration, J. Immunol., 163, 5029, 10.4049/jimmunol.163.9.5029 · DOI
  51. Lefort, 2012, Neutrophil arrest by LFA-1 activation, Front. Immunol., 3, 157, 10.3389/fimmu.2012.00157 · DOI
  52. Phillipson, 2006, Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade, J. Exp. Med., 203, 2569, 10.1084/jem.20060925 · DOI

Cited by

See all 58

Metadata

Authors
Zehrer, A., Pick, R., Salvermoser, M., Boda, A., Miller, M., Stark, K., Weckbach, L. T., Walzog, B., & Begandt, D.
Affiliations
  • Walter Brendel Centre of Experimental Medicine, University Hospital and Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, LMU Munich , 82152 Planegg-Martinsried ,
  • Medizinische Klinik und Poliklinik I, Klinikum der Universität, Ludwig-Maximilians-Universität München , 81377 Munich ,
ORCID
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2018-09
Volume
201
Issue
6
Pages
1748-1764
ISSN
  • 0022-1767
  • 1550-6606
DOI
10.4049/jimmunol.1701400
Cited by
58
References
0
Language
en
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: A Fundamental Role of Myh9 for Neutrophil Migration in Innate Immunity. https://app.cochat.ai/papers/10.4049/jimmunol.1701400
The paper
Zehrer, A., Pick, R., Salvermoser, M., Boda, A., Miller, M., Stark, K., Weckbach, L. T., Walzog, B., & Begandt, D. (2018). A Fundamental Role of Myh9 for Neutrophil Migration in Innate Immunity. The Journal of Immunology. https://doi.org/10.4049/jimmunol.1701400