The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Preferential Cell Death of CD8+ Effector Memory (CCR7−CD45RA−) T Cells by Hydrogen Peroxide-Induced Oxidative Stress
Open accessAbstract
T cells are used in many cell-based cancer treatments. However, oxidative stress that is induced during various chronic inflammatory conditions, such as cancer, can impair the immune system and have detrimental effects on T cell function. In this study, we have investigated the sensitivity of different human T cell subsets to H2O2-induced oxidative stress. We showed that central memory (CD45RA−CCR7+) and effector memory (CD45RA−CCR7−) T cells are more sensitive to H2O2 as compared with naive (CD45RA+CCR7+) T cells. Furthermore, the study showed that CD8+ effector memory T cells are more sensitive to low levels of H2O2 (5 μM) compared with other types of T cells investigated. H2O2-exposed CD45RO+ T cells showed mitochondrial depolarization prior to caspase 3 activity. Moreover, the pan-caspase inhibitor z-Val-Ala-Asp(OMe)-fluoromethylketone rescued cells from death. These experiments suggest that H2O2-induced cell death of CD45RO+ T cells acts via the mitochondrial pathway and that caspase involvement is needed. This study suggests that oxidative stress in cancer patients can be disadvantageous for T cell-based adoptive cell transfer therapies, since effector memory T cells are the primary phenotype of the cells administered.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“T cells expressing cell-surface proteins associated with differentiation or “senescence” (e.g., CD57 and KLRG1) are more susceptible to H2O2 induced apoptosis than total lymphocytes (Wang and Lin 2010) and cells of a naïve phenotype (e.g., CD45RA + CCR7+) in vitro (Takahashi et al. 2005).”
From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Takahashi et al. 2005
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
Sallusto, F., Lenig, D., Förster, R., Lipp, M., & Lanzavecchia, A. (1999) · Nature · doi:10.1038/44385 · Cited by 5,846
Central Memory and Effector Memory T Cell Subsets: Function, Generation, and Maintenance
Sallusto, F., Geginat, J., & Lanzavecchia, A. (2004) · Annual Review of Immunology · doi:10.1146/annurev.immunol.22.012703.104702 · Cited by 3,067
Cancer Regression and Autoimmunity in Patients After Clonal Repopulation with Antitumor Lymphocytes
Dudley, M. E., Wunderlich, J. R., Robbins, P. F., Yang, J. C., Hwu, P., Schwartzentruber, D. J., Topalian, S. L., Sherry, R., Restifo, N. P., Hubicki, A. M., et al. (2002) · Science · doi:10.1126/science.1076514 · Cited by 2,823
The mitochondrial permeability transition
Zoratti, M., & Szabó, I. (1995) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(95)00003-a · Cited by 2,410
Almand, B., Clark, J. I., Nikitina, E., van Beynen, J., English, N. R., Knight, S. C., Carbone, D. P., & Gabrilovich, D. I. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.1.678 · Cited by 1,340
Schmielau, J., & Finn, O. J. (2001) · PubMed · Cited by 867
Respiratory burst in human neutrophils
Dahlgren, C., & Karlsson, A. (1999) · Journal of Immunological Methods · doi:10.1016/s0022-1759(99)00146-5 · Cited by 810
The Rel/NF-κB Family Directly Activates Expression of the Apoptosis Inhibitor Bcl-x L
Chen, C., Edelstein, L. C., & Gélinas, C. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.8.2687-2695.2000 · Cited by 760
Zong, W.-X., Edelstein, L., Chen, C.-L., Bash, J., & Gélinas, C. (1999) · Genes & Development · doi:10.1101/gad.13.4.382 · Cited by 738
Dual regulation of caspase activity by hydrogen peroxide: implications for apoptosis
Hampton, M. B., & Orrenius, S. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01068-5 · Cited by 663
Flagged first, then by citations
Research works printed but not linked: 58
- 1979, Adv. Exp. Med. Biol., 53
- 1999, J. Immunol. Methods, 3
- 2003, Int. J. Obes. Relat. Metab. Disord., S12
- 1996, Neuron, 345
- 1995, Free Radical Res., 389
- 1994, J. Immunol., 4880
- 1998, Clin. Exp. Immunol., 242
- 1998, J. Immunol., 1354
- 2001, J. Immunol., 2595
- 2000, J. Immunol., 2170
- 1997, J. Immunol., 2973
- 2002, Antioxid. Redox Signal., 543
- 1997, Free Radical Biol. Med., 775
- 2001, Cancer Res., 4756
- 2004, Cancer Immunol. Immunother., 879
- 1995, J. Exp. Med., 1881
- 1996, Proc. Natl. Acad. Sci. USA, 13119
- 1996, Eur. J. Immunol., 1308
- 1996, J. Immunol., 42
- 1998, J. Immunol., 5729
- 1999, Nature, 708
- 2004, Annu. Rev. Immunol., 745
- 1999, Immunity, 735
- 2003, Diabetes, 1423
- 1998, Blood, 4808
- 1997, FEBS Lett., 552
- 1999, Oncogene, 747
- 1998, FEBS Lett., 351
- 1995, Biochim. Biophys. Acta, 139
- 2004, Cell Death Differ., 416
- 2002, Immunol. Lett., 107
- 1993, J. Exp. Med., 427
- 2002, Immunity, 759
- 1994, Eur. J. Immunol., 892
- 1999, J. Immunol., 4527
- 2000, J. Immunol., 3950
- 2000, Mol. Cell. Biol., 2687
- 1999, Genes Dev., 400
- 2000, J. Immunol., 1743
- 1999, Mol. Cell. Biol., 5923
- 1999, Genes Dev., 382
- 1998, Infect. Immun., 499
- 1998, Blood, 585
- 1993, Cancer Res., 5610
- 1995, Int. J. Cancer, 765
- 1996, Clin. Cancer Res., 161
- 1996, Clin. Cancer Res., 1825
- 1998, Int. J. Cancer, 16
- 1999, Clin. Cancer Res., 1263
- 2004, Clin. Cancer Res., 5101
- 2000, Clin. Cancer Res., 1351
- 1999, Cancer Res., 2950
- 2003, Nat. Immunol., 957
- 2000, Blood, 3838
- 2001, J. Immunol., 678
- 2002, Science, 850
- 2005, Blood, 241
- 2002, Clin. Cancer Res., 1772
Cited by
Sorted by citations
Items 101–101 of 101
CD8+ T cells in atherosclerosis and coronary artery disease
Tandon, I., yosri, M., Soliman, H., Khalifa, A. M., Ley, K. F., & Abdelsamed, H. A. (2026) · Nature Reviews Cardiology · doi:10.1038/s41569-026-01295-7 · Cited by 1
Metadata
- Authors
- Takahashi, A., Hanson, M. G. V., Norell, H. R., Havelka, A. M., Kono, K., Malmberg, K.-J., & Kiessling, R. V. R.
- Affiliations
- Karolinska Institutet (SE)
- Department of Oncology and Pathology, Immune and Gene Therapy Laboratory, Cancer Center Karolinska, Karolinska Institutet , Stockholm ,
- University of Yamanashi (JP)
- Karolinska University Hospital (SE)
- Corresponding author
- Takahashi, Akihiro
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-05
- Volume
- 174
- Issue
- 10
- Pages
- 6080-6087
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2139536543
- Cited by
- 101
- References
- 62
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 9 · 2023: 3 · 2022: 4 · 2021: 8 · 2020: 5 · 2019: 3 · 2018: 6 · 2017: 3 · 2016: 7 · 2015: 4 · 2014: 5 · 2013: 9 · 2012: 8
- FWCI
- 1.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Commission
- Cancerfonden
- Karolinska Institutet
- National Institutes of Health
- National Cancer Institute (R01CA102280, CA 102280)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- hydrogen peroxide, oxidative stress, T cell death, CD8+ effector memory T cells, central memory T cells, naive T cells, mitochondrial depolarization, caspase-3, z-VAD-fmk, adoptive cell transfer, T cell subsets, cancer immunotherapy
- MeSH terms
- Amino Acid Chloromethyl Ketones, Cells, Cultured, Cytotoxicity, Immunologic, Dose-Response Relationship, Immunologic, Growth Inhibitors, Humans, Hydrogen Peroxide, Immunologic Memory, Intracellular Membranes, Membrane Potentials, Mitochondria, Permeability, T-Lymphocytes, Cytotoxic, Cell Death, Leukocyte Common Antigens, Oxidative Stress, Receptors, Chemokine, Receptors, CCR7, Caspase Inhibitors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
