The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Proteomic Analysis of Microglia-Derived Exosomes: Metabolic Role of the Aminopeptidase CD13 in Neuropeptide Catabolism
Abstract
Vesicle transport is a fundamental mechanism of communication in the CNS. In this study we characterized a novel type of vesicle released by murine brain microglial cells: microglial exosomes. Analysis of their protein content identified several enzymes, chaperones, tetraspanins, and membrane receptors previously reported in B cells and dendritic cell-derived exosomes. Additionally, microglia-derived exosomes expressed the aminopeptidase CD13 and the lactate transporter MCT-1. Exosomal CD13 was metabolically active in cleaving leucine- and methionine-enkephalins peptides by releasing the N-terminal tyrosine. Cleaved neuropeptides were unable to bind to the neuronal opioid receptor as assessed by cAMP response. Microglial exosomal vesicles may represent an important, previously unrecognized, cellular communication system in an organ in which cell motility is highly restricted.
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“Aminopeptidase CD13 is an important characteristic protein of exosomes derived from microglia,44”
From Microglia secrete miR-146a-5p-containing exosomes to regulate neurogenesis in depression (2022) · cites this paper as [44]
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Metadata
- Authors
- Potolicchio, I., Carven, G. J., Xu, X., Stipp, C., Riese, R. J., Stern, L. J., & Santambrogio, L.
- Affiliations
- Albert Einstein College of Medicine (US)
- University of Massachusetts Chan Medical School (US)
- Dana-Farber Cancer Institute (US)
- Brigham and Women's Hospital (US)
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-08
- Volume
- 175
- Issue
- 4
- Pages
- 2237-2243
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1524589668
- Cited by
- 365
- References
- 37
- Citations by year
- 2026: 11 · 2025: 6 · 2024: 18 · 2023: 22 · 2022: 32 · 2021: 31 · 2020: 30 · 2019: 29 · 2018: 16 · 2017: 18 · 2016: 29 · 2015: 13 · 2014: 16 · 2013: 14 · 2012: 22
- FWCI
- 3.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Funders
- National Institutes of Health (5k08ai001555-03)
- National Cancer Institute (CA 86712, R01CA086712)
- National Institute of Allergy and Infectious Diseases (K08AI01555, R01-AI38996, R01AI048832, R01AI038996)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- CD13, aminopeptidase, enkephalin, opioid receptors, MCT1, lactate transporters, tetraspanins, cell surface receptors, molecular chaperones
- MeSH terms
- Animals, Antigens, Differentiation, B-Lymphocyte, Histocompatibility Antigens Class II, Cathepsins, Cell Communication, Cell Line, Neuropeptides, Endosomes, Biomarkers, Microglia, CD13 Antigens, Proteome, Cytoplasmic Vesicles, Cell Line, Tumor, Mice
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