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Cited by 31

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The Use of HRP in Decolorization of Reactive Dyes and Toxicological Evaluation of Their Products

da Silva, Michelle Reis, de Sá, Lívian Ribeiro Vasconcelos, Russo, Carlos, Scio, Elita, Ferreira-Leitão, Viridiana Santana·2010·Enzyme Research

Abstract

This work studied the potential use of horseradish peroxidase (HRP) in the decolorization of the following textile dyes: Drimarene Blue X-3LR (DMBLR), Drimarene Blue X-BLN (DMBBLN), Drimarene Rubinol X-3LR (DMR), and Drimarene Blue CL-R (RBBR). Dyes were individually tested in the reaction media containing 120 mg⋅L-1, considering the following parameters: temperature (20–45°C), H 2 O 2 concentration (0–4.44 mmol⋅L-1), and reaction time (5 minutes, 1 and 24 h). The following conditions: 35°C, 0.55 mmol⋅L-1, and 1h, provided the best set of results of color removal for DMBLR (99%), DMBBLN (77%), DMR (94%), and RBBR (97%). It should be mentioned that only 5 minutes of reaction was enough to obtain 96% of decolorization for DMBLR and RBBR. After the decolorization reactions of DMBLR, DMR, and RBBR, it was possible to observe the reduction of Artemia salina mortality and the no significant increase in toxicity for the products generated from DMBBLN.

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Registry

Crossref · 10.4061/2010/703824

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Figures

Figure 1 Chemical structures of the dyes DMBLR, DMBBLN, DMR e RBBR [33–35].
Figure 1 Chemical structures of the dyes DMBLR, DMBBLN, DMR e RBBR [33–35].CC BY · PubMed Central
Figure 2 Influence of temperature on the decolorization of textile dyes. Reaction conditions: enzyme's activity = 3.5 U·mL−1, dyes concentrations = 120 mg·L−1, H2O2 = 0.55 mmol·L−1, and reaction time = 1 h.
Figure 2 Influence of temperature on the decolorization of textile dyes. Reaction conditions: enzyme's activity = 3.5 U·mL−1, dyes concentrations = 120 mg·L−1, H2O2 = 0.55 mmol·L−1, and reaction time = 1 h.CC BY · PubMed Central
Figure 3 Influence of H2O2 concentration on the decolorization of textile dyes. Reaction conditions: enzyme's activity = 3.5 U·mL−1, dyes concentrations = 120 mg·L−1, temperature = 35°C and, reaction time = 1 h.
Figure 3 Influence of H2O2 concentration on the decolorization of textile dyes. Reaction conditions: enzyme's activity = 3.5 U·mL−1, dyes concentrations = 120 mg·L−1, temperature = 35°C and, reaction time = 1 h.CC BY · PubMed Central
Figure 4 Influence of reaction time on the decolorization of textile dyes. Reaction conditions: enzyme's activity = 3.5 U·mL−1, dyes concentrations = 120 mg·L−1, temperature = 35°C, and H2O2 concentration = 0.55 mmol·L−1.
Figure 4 Influence of reaction time on the decolorization of textile dyes. Reaction conditions: enzyme's activity = 3.5 U·mL−1, dyes concentrations = 120 mg·L−1, temperature = 35°C, and H2O2 concentration = 0.55 mmol·L−1.CC BY · PubMed Central
Figure 5 Percentage of mortality of Artemia salina according to the concentration of reaction media containing each dye—DMBLR, DMR, RBBR, and DMBBLN—before enzymatic action or their respective products after the enzymatic treatment with HRP.
Figure 5 Percentage of mortality of Artemia salina according to the concentration of reaction media containing each dye—DMBLR, DMR, RBBR, and DMBBLN—before enzymatic action or their respective products after the enzymatic treatment with HRP.CC BY · PubMed Central

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Research works printed but not linked: 1

  1. 1982, Planta Medica, 45, 31

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Metadata

Authors
da Silva, M. R., de Sá, L. R. V., Russo, C., Scio, E., & Ferreira-Leitão, V. S.
Venue
Enzyme Research
Publisher
Wiley
Published
2010-01-20
Volume
2010
Pages
1-7
ISSN
2090-0414
Cited by
31
References
37
Language
en
Funders
Conselho Nacional de Desenvolvimento Científico e Tecnológico

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