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Cited by 11Spinal Cord Injury ResearchMesenchymal stem cell researchNerve injury and regeneration

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Transplantation of neurotrophin-3-transfected bone marrow mesenchymal stem cells for the repair of spinal cord injury

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Dong, Yuzhen, Yang, Libin, Yang, Lin, Zhao, Hongxing, Zhang, Chao, Wu, Dapeng·2014·Neural Regeneration Research
DOI 10.4103/1673-5374.139478PMC PMC4192969
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Abstract

Bone marrow mesenchymal stem cell transplantation has been shown to be therapeutic in the repair of spinal cord injury. However, the low survival rate of transplanted bone marrow mesenchymal stem cells in vivo remains a problem. Neurotrophin-3 promotes motor neuron survival and it is hypothesized that its transfection can enhance the therapeutic effect. We show that in vitro transfection of neurotrophin-3 gene increases the number of bone marrow mesenchymal stem cells in the region of spinal cord injury. These results indicate that neurotrophin-3 can promote the survival of bone marrow mesenchymal stem cells transplanted into the region of spinal cord injury and potentially enhance the therapeutic effect in the repair of spinal cord injury.

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Figure 1 The number of bone marrow mesenchymal stem cells (BMSCs) transfected with or without neurotrophin-3 (NT-3) during culture. After 3 days, the number of BMSCs transfected with NT-3 was significantly higher than that of non-transfected with NT-3 (P < 0.05). The data were expressed as mean ± SD and analyzed using two-sample t-test.
Figure 1 The number of bone marrow mesenchymal stem cells (BMSCs) transfected with or without neurotrophin-3 (NT-3) during culture. After 3 days, the number of BMSCs transfected with NT-3 was significantly higher than that of non-transfected with NT-3 (P < 0.05). The data were expressed as mean ± SD and analyzed using two-sample t-test.CC BY-NC-SA · PubMed Central
Figure 2 Expression of enhanced green fluorescent protein (EGFP; green fluorescence) in the bone marrow mesenchymal stem cells (BMSCs) transplanted into the injured spinal cord at 4 weeks after transplantation (fluorescence microscope, × 200). EGFP expression was significantly greater in the BMSCs + neurotrophin-3 group (C) than in the spinal cord injury (A) and BMSCs groups (B). The expression was lowest in the spinal cord injury group.
Figure 2 Expression of enhanced green fluorescent protein (EGFP; green fluorescence) in the bone marrow mesenchymal stem cells (BMSCs) transplanted into the injured spinal cord at 4 weeks after transplantation (fluorescence microscope, × 200). EGFP expression was significantly greater in the BMSCs + neurotrophin-3 group (C) than in the spinal cord injury (A) and BMSCs groups (B). The expression was lowest in the spinal cord injury group.CC BY-NC-SA · PubMed Central
Figure 3 Apoptosis of motor neurons in spinal cord anterior horn at 4 weeks after transplantation (TUNEL staining, × 400). (A–C) Spinal cord injury, bone marrow mesenchymal stem cells (BMSCs), and BMSCs + neurotrophin-3 (NT-3) groups. Apoptotic cells in the spinal cord anterior horn exhibited yellow-brown nuclei. The number of apoptotic cells was lowest in the BMSCs + NT-3 group but highest in the spinal cord injury group.
Figure 3 Apoptosis of motor neurons in spinal cord anterior horn at 4 weeks after transplantation (TUNEL staining, × 400). (A–C) Spinal cord injury, bone marrow mesenchymal stem cells (BMSCs), and BMSCs + neurotrophin-3 (NT-3) groups. Apoptotic cells in the spinal cord anterior horn exhibited yellow-brown nuclei. The number of apoptotic cells was lowest in the BMSCs + NT-3 group but highest in the spinal cord injury group.CC BY-NC-SA · PubMed Central

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Research works printed but not linked: 32

  1. Alexanian AR, Maiman DJ, Kurpad SN, Gennarelli TA (2008) In vitro and in vivo characterization of neurally modified mesenchymal stem cells induced by epigenetic modifiers and neural stem cell environment. Stem Cells Dev 17:1123-1130. · DOI
  2. Alto LT, Havton LA, Conner JM, Hollis ER 2 nd , Blesch A, Tuszynski MH (2009) Chemotropic guidance facilitates axonal regeneration and synapse formation after spinal cord injury. Nat Neurosci 12:1106-1113. · DOI
  3. Awad BI, Carmody MA, Steinmetz MP (2013) Potential role of growth factors in the management of spinal cord injury. World Neurosurg doi: 10.1016/j.wneu.2013.01.042. · DOI
  4. Boyce VS, Park J, Gage FH, Mendell LM (2012) Differential effects of brain-derived neurotrophic factor and neurotrophin-3 on hindlimb function in paraplegic rats. Eur J Neurosci 35:221-232. · DOI
  5. Dasari VR, Veeravalli KK, Dinh DH (2014) Mesenchymal stem cells in the treatment of spinal cord injuries: A review. World J Stem Cells 6:120-133. · DOI
  6. David S, Kroner A (2011) Repertoire of microglial and macrophage responses after spinal cord injury. Nat Rev Neurosci 12:388-399. · DOI · Paper page
  7. Fan CG, Zhang QJ, Zhou JR (2011) Therapeutic potentials of mesenchymal stem cells derived from human umbilical cord. Stem Cell Rev Rep 7:195-207. · DOI
  8. Fan J, Xiao Z, Zhang H,Chen B, Tang G, Hou X, Ding W, Wang B, Zhang P, Dai J, Xu R (2010) Linear ordered collagen scaffolds loaded with collagen-binding neurotrophin-3 promote axonal regeneration and partial functional recovery after completespinal cord transection. J Neurotrauma 27:1671-1683. · DOI
  9. Gou S,Wang C,Liu T,Wu H, Xiong J, Zhou F, Zhao G (2010) Spontaneous differentiation of murine bone marrow-derived mesenchymal stem cells into adipocytes without malignant transformation after long-term culture. Cells Tissues Organs 191:185-192. · DOI
  10. Gray M, Palispis W, Popovich K, Popovich PG, van Rooijen N, Gupta R (2007) Macrophage depletion alters the blood-nerve barrier without affecting Schwann cell function after neural injury. J Neurosci Res 85:766-777. · DOI
  11. Harvey RL, Chopp M (2003) The therapeutic effects of cellular therapy for functional recovery after brain injury. Phys Med Rehabil Clin N Am 14:S143-151. · DOI
  12. Huang EJ, Reichardt LF (2001) Neurotrophins: roles in neuronal development and function. Annu Rev Neurosci 24:677-736. · DOI · Paper page
  13. Ide C, Nakai Y, Nakano N, Seo T-B, Yamada Y, Endo K, Noda T, Saito F, Suzuki Y, Fukushima M, Nakatani T (2010) Bone marrow stromal cell transplantation for treatment of sub-acute spinal cord injury in the rat. Brain Res 1332:32-47. · DOI
  14. Kan EM, Ling EA, Lu J (2010) Stem cell therapy for spinal cord injury. Curr Med Chem 17:4492-4510. · DOI
  15. Langworthy MM, Appel B (2012) Schwann cell myelination requires Dynein function. Neural Dev 7:37. · DOI
  16. Lin WP, Chen XW, Zhang LQ, Wu CY, Huang ZD, Lin JH (2013) Effect of neuroglobin genetically modified bone marrow mesenchymal stem cells transplantation onspinal cord injury in rabbits. PLoS One 8:e63444. · DOI
  17. Naghdi M, Tiraihi T, Mesbah-Namin SA, Arabkharadmand J, Kazemi H, Taheri T (2013) Improvement of contused spinal cord in rats by cholinergic-like neuron therapy. Iran Red Crescent Med J 15:127-135. · DOI
  18. Oda Y, Tani K, Isozaki A, Haraguchi T, Itamoto K, Nakazawa H, Taura Y (2014) Effects of polyethylene glycol administration and bone marrow stromal cell transplantation therapy in spinal cord injury mice. J Vet Med Sci 76:415-421. · DOI
  19. Panepucci RA, Siufi JL, Silva WA Jr, Proto-Siquiera R, Neder L, Orellana M (2004) Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells. Stem Cells 14:1263-1278. · DOI
  20. Saito F, Nakatani T, Iwase M, Maeda Y. Murao Y, Suzuki Y, Fukushima M, Ide C (2012) Administration of cultured autologous bone marrow stromal cells into cerebrospinal fluid in spinal injury patients: a pilot study. Restor Neurol Neurosci 30:127-136. · DOI
  21. Smith RR, Brown EH, Shum-Siu A, Whelan A, Burke DA, Benton RL, Magnuson DS (2009) Swim training initiated acutely after spinal cord injury is ineffective and induces extravasation in and around the epicenter. J Neurotrauma 26:1017-1027. · DOI
  22. Solev IN, Balabanyan VY, Volchek IA, Elizarova OS, Litvinova SA, Garibova TL, Voronina TA (2013) Involvement of BDNF and NGF in the mechanism of neuroprotective effect of human recombinant erythropoietin nanoforms. Bull Exp Biol Med 155:242-244. · DOI
  23. Thomas SL, Gorassini MA (2005) Increases in corticospinal tract function by treadmill training after incomplete spinal cord injury. J Neurophysiol 94:2844-2855. · DOI
  24. Thomson SR, Nahon JE, Mutsaers CA, Thomson D, Hamilton G, Parson SH, Gillingwater TH (2012) Morphological characteristics of motor neurons do not determine their relative susceptibility to degeneration in a mouse model of severe spinal muscular atrophy. PLoS One 7:e52605. · DOI
  25. Vawda R, Fehlings MG (2013) Mesenchymal cells in the treatment of spinal cord injury: current & future perspectives. Curr Stem Cell Res Ther 8:25-38. · DOI
  26. Wang X, Li Y, Gao Y, Chen X, Yao J, Lin W, Chen Y, Liu J, Yang Y, Wang X (2013) Combined use of spinal cord-mimicking partition type scaffold architecture and neurotrophin-3 for surgical repair of completely transected spinal cord in rats. J Biomater Sci Polym Ed 24:927-939. · DOI
  27. Wood MD, Moore AM, Hunter DA, Tuffaha S, Borschel GH, Mackinnon SE, Sakiyama-Elbert SE (2009) Affinity-based release of glial-derived neurotrophic factor from fibrin matrices enhances sciatic nerve regeneration. Acta Biomater 5:959-968. · DOI
  28. Yang Z, Duan H, Mo L, Qiao H, Li X (2010) The effect of the dosage of NT-3/chitosan carriers on the proliferation and differentiation of neural stem cells. Biomaterials 31:4846-4854. · DOI
  29. Yip PK, Malaspina A (2012) Spinal cord trauma and the molecular point of no return. Mol Neurodegener 7:6. · DOI
  30. Zhang D, He X (2014) A meta-analysis of the motion function through the therapy of spinal cord injury with intravenous transplantation of bone marrow mesenchymal stem cells in rats. PLoS One 9:e93487. · DOI
  31. Zhang L, Ma Z, Smith GM, Wen X, Pressman Y, Wood PM, Xu XM (2009) GDNF-enhanced axonal regeneration and myelination following spinal cord injury is mediated by primary effects on neurons. Glia 57:1178-1191. · DOI
  32. Zhang YJ, Zhang W, Lin CG, Ding Y, Huang SF, Wu JL, Li Y, Dong H, Zeng YS (2012) Neurotrophin-3 gene modified mesenchymal stem cells promote remyelination and functional recovery in the demyelinated spinal cord of rats. J Neurol Sci 313:64-74. · DOI

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Metadata

Authors
Dong, Y., Yang, L., Yang, L., Zhao, H., Zhang, C., & Wu, D.
Affiliations
First Affiliated Hospital of Xinxiang Medical University (CN)
Corresponding author
Dong, Yuzhen
ORCID
Venue
Neural Regeneration Research
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2014
Volume
9
Issue
16
Pages
1520
ISSN
1673-5374 (ISSN-L)
DOI
10.4103/1673-5374.139478
PMC
PMC4192969
OpenAlex
W1985088489
Cited by
11
References
0
Citations by year
2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 1
FWCI
0.99
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Neural Regeneration Research (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • 中国神经再生研究：英文版
Funders
Xinxiang Medical University
Topics
  • Health Sciences › Medicine › Clinical Neurology › Spinal Cord Injury Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
Keywords
neurotrophin-3, bone marrow mesenchymal stem cells, spinal cord injury, transfection, stem cell transplantation, stem cell survival, motor neuron survival, functional recovery

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CoChat. (2026). Citation check: Transplantation of neurotrophin-3-transfected bone marrow mesenchymal stem cells for the repair of spinal cord injury. https://app.cochat.ai/papers/10.4103/1673-5374.139478
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Dong, Y., Yang, L., Yang, L., Zhao, H., Zhang, C., & Wu, D. (2014). Transplantation of neurotrophin-3-transfected bone marrow mesenchymal stem cells for the repair of spinal cord injury. Neural Regeneration Research. https://doi.org/10.4103/1673-5374.139478