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A RAPID PROCEDURE FOR PURINE MEASUREMENT AND ITS USE FOR ESTIMATING NET RUMINAL PROTEIN SYNTHESIS
Abstract
A rapid method for separation and quantitation of purines was applied to ruminal and intestinal digesta for estimating net microbial protein synthesis in the rumen. The procedure combines standard literature methods for hydrolysis of nucleotides by perchloric acid followed by precipitation of free purines with silver nitrate to separate the purines from interfering compounds. Acid resolubilized purines were quantitated spectrophotometrically at 260 nm. Microbial protein was estimated by the ratio of purines to N of isolated bacteria. The procedure is rapid, simple, precise and not costly. Duodenal passage of microbial N estimated by this procedure for steers fed semipurified and purified diets containing no protein was highly correlated (R 2 = 0.98; P < 0.01) with duodenal passage of tungstic acid precipitable N. Results indicate that purines may be useful as a marker for quantitating microbial protein. Key words: Purine, RNA, DNA, microbial protein
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Cited by
Huws, S. A., Creevey, C. J., Oyama, L. B., Mizrahi, I., Denman, S. E., Popova, M., Muñoz-Tamayo, R., Forano, E., Waters, S. M., Hess, M., et al. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02161 · Cited by 487
Invited Review: Nitrogen in Ruminant Nutrition: A Review of Measurement Techniques
Hristov, A. N., Bannink, A., Crompton, L. A., Huhtanen, P., Kreuzer, M., McGee, M., Nozière, P., Reynolds, C. K., Bayat, A. R., Yáñez-Ruiz, D. R., et al. (2019) · Journal of Dairy Science · doi:10.3168/jds.2018-15829 · Cited by 283
Microbial protein supply from the rumen
Dewhurst, R. J., Davies, D. R., & Merry, R. J. (2000) · Animal Feed Science and Technology · doi:10.1016/s0377-8401(00)00139-5 · Cited by 276
Chen, X. B., Chen, Y. K., Franklin, M. F., Ørskov, E. R., & Shand, W. J. (1992) · Journal of Animal Science · doi:10.2527/1992.7051534x · Cited by 260
Johnson, L. M., Harrison, J. H., Davidson, D., Mahanna, W. C., & Shinners, K. (2003) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(03)73601-7 · Cited by 68
Metadata
- Authors
- ZINN, R. A., & OWENS, F. N.
- Venue
- Canadian Journal of Animal Science
- Publisher
- Canadian Science Publishing
- Published
- 1986-03-01
- Volume
- 66
- Issue
- 1
- Pages
- 157-166
- ISSN
- 0008-3984 (ISSN-L)
- 1918-1825
- OpenAlex
- W1991995635
- Cited by
- 816
- References
- 0
- FWCI
- 18.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A RAPID PROCEDURE FOR PURINE MEASUREMENT AND ITS USE FOR ESTIMATING NET RUMINAL PROTEIN SYNTHESIS. https://app.cochat.ai/papers/10.4141/cjas86-017
- The paper
- ZINN, R. A., & OWENS, F. N. (1986). A RAPID PROCEDURE FOR PURINE MEASUREMENT AND ITS USE FOR ESTIMATING NET RUMINAL PROTEIN SYNTHESIS. Canadian Journal of Animal Science. https://doi.org/10.4141/cjas86-017
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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