H2B (Ser10) Phosphorylation is Induced during Apoptosis and Meiosis in S. cerevisiae
Open accessAbstract
The nucleosome, composed of an octamer of highly conserved histone proteins and associated DNA, is the fundamental unit of eukaryotic chromatin. How arrays of nucleosomes are folded into higher-order structures, and how the dynamics of such compaction are regulated, are questions that remain largely unanswered. Our recent studies demonstrated that phosphorylation of histone H2B is necessary to induce cell death that exhibits phenotypic hallmarks of apoptosis including DNA fragmentation and chromatin condensation in yeast (serine 10)1 and in mammalian cells (serine 14)2. In this article, we extend these findings by uncovering a role for H2B phosphorylation at serine 10 (Ser10) in another biological event that is associated with dramatic alterations in higher-order chromatin structure, meiosis. Our data show strong staining, indicative of H2B (Ser10) phosphorylation, during the pachytene stage of yeast meiotic prophase. These data broaden the use of this phosphorylation mark in chromatin remodeling that closely correlates with chromatin compaction. How phosphorylation marks are translated into meaningful downstream events during processes as diverse as apoptosis and meiosis remain a challenge for future studies.
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PCD can also occur during meiosis of diploid yeast cells, where it is regulated by histone H2B phosphorylation.
“PCD can also occur during meiosis of diploid yeast cells, where it is regulated by histone H2B phosphorylation (Ahn et al., 2006; Ahn et al., 2005).”
From Programmed cell death in aging (2015) · cites this paper as Ahn et al., 2005
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- Authors
- Ahn, S.-H., Henderson, K. A., Keeney, S., & Allis, C. D.
- Affiliations
- Rockefeller University (US)
- Memorial Sloan Kettering Cancer Center (US)
- Venue
- Cell Cycle
- Publisher
- Informa UK Limited
- Published
- 2005-04-13
- Volume
- 4
- Issue
- 6
- Pages
- 780-783
- ISSN
- 1538-4101 (ISSN-L)
- 1551-4005
- OpenAlex
- W2045180043
- Cited by
- 53
- References
- 0
- Citations by year
- 2026: 2 · 2022: 3 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 2.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cell Cycle (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (GM-40922, R01GM040922)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- apoptosis, meiosis, Saccharomyces cerevisiae, chromatin condensation, chromatin remodeling, pachytene, histone modifications
- MeSH terms
- Animals, Histones, Meiosis, Phosphorylation, Saccharomyces cerevisiae, Time Factors, Apoptosis
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