The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
A HIGHLY SENSITIVE AND SPECIFIC REAL-TIME PCR ASSAY FOR THE DETECTION OF SPOTTED FEVER AND TYPHUS GROUP RICKETTSIAE
Abstract
A highly specific real-time polymerase chain reaction (PCR) assay was developed to detect spotted fever and typhus group rickettsiae using the citrate synthase gene as the target. The assay amplified rickettsial members of the spotted fever and typhus group including Rickettsia akari, R. australis, R. conorii, R. honei, “R. marmionii,” R. sibirica, R. rickettsii, R. typhi, and R. prowazekii. The ancestral group rickettsia, R. bellii, did not produce a positive reaction, nor did other members of the order Rickettsiales or any non-rickettsial bacteria. The assay had a sensitivity of one target copy number per reaction as determined by serial dilutions of a plasmid containing a spotted fever group target sequence. This quantitative assay is useful for the enumeration of rickettsiae in clinical specimens and the diagnosis of rickettsial illnesses, when rickettsial numbers are very low.
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Cites
Basic Methods in Molecular Biology
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The genome sequence of Rickettsia prowazekii and the origin of mitochondria
Andersson, S. G. E., Zomorodipour, A., Andersson, J. O., Sicheritz-Pontén, T., Alsmark, U. C. M., Podowski, R. M., Näslund, A. K., Eriksson, A.-S., Winkler, H. H., & Kurland, C. G. (1998) · Nature · doi:10.1038/24094 · Cited by 1,697
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Rickettsioses as paradigms of new or emerging infectious diseases
Raoult, D., & Roux, V (1997) · Clinical Microbiology Reviews · doi:10.1128/cmr.10.4.694 · Cited by 927
ROUX, V., RYDKINA, E., EREMEEVA, M., & RAOULT, D. (1997) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-47-2-252 · Cited by 562
Multiplex Real-Time PCR for Detection of Anaplasma phagocytophilum and Borrelia burgdorferi
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Towards a Phylogeny and Definition of Species at the Molecular Level within the Genus Mycobacterium
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Cited by
Rickettsia helvetica in Patient with Meningitis, Sweden, 2006
Nilsson, K., Elfving, K., & Påhlson, C. (2010) · Emerging Infectious Diseases · doi:10.3201/eid1603.090184 · Cited by 145
Metadata
- Authors
- STENOS, J., GRAVES, S. R., & UNSWORTH, N. B.
- Venue
- The American Journal of Tropical Medicine and Hygiene
- Published
- 2005-12
- Volume
- 73
- Issue
- 6
- Pages
- 1083-1085
- ISSN
- 0002-9637 (ISSN-L)
- OpenAlex
- W2123539550
- Cited by
- 299
- References
- 13
- FWCI
- 2.54
- Type
- journal article
- Language
- en
- Open access
- closed
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