Acid fast bacilli in aspiration smears from tuberculous patients
Open accessAbstract
Fine-Needle aspiration (FNA) has been used in the diagnosis of tuberculous adenitis or extrapulmonary tuberculosis of extranodal sites for more than ten years in the Tikur Anbessa Teaching Hospital, Department of Pathology, Faculty of Medicine, Addis Ababa University. The purpose of this study was to see the correlation between the cytomorphologic diagnosis and the subsequent demonstration of acid fast bacilli in the smear and also to know the overall acid fast bacilli (AFB) positivity. A cross sectional study, involving a total of 205 consecutive patients diagnosed to have tuberculosis by cytomorphology (FNA) between mid 1996 and early 1997 were included in the study. The May-Grunwald Giemsa stained smears were classified into three categories: Epithelioid granuloma without caseous necrosis, Epithelioid granuloma with caseous necrosis, Abscess and/or Caseous necrosis. There were 131 (63.9%) cases with granuloma with caseous necrosis, 67(32.7%) cases with abscess and/or caseous necrosis , and only seven (3.4%) cases with granuloma without caseous necrosis. The AFB positivity as stained by Ziehl Neelsen stain was 99 (75.6%) in granuloma with caseous necrosis, 46(68.7%) in tuberculous abscess and/or caseous necrosis and 2(28.5%) in granuloma without necrosis. The overall acid fast positivity was 71.7%. This study confirmed that FNA alone is helpful enough in making a diagnosis of tuberculosis in most of the cases. We recommend that the evaluation of all fine needle aspiration samples from suspected cases of tuberculosis should include staining for AFB for confirmation and in particular for cases of abscess and necrosis as part a diagnostic step. However, culture of the aspirate is still required when the smears are AFB negative and cytologically equivocal whenever it is available. (Ethiopian Journal of Health Development: 2000, 14(1): 99-104)
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
ZN smear microscopy has low sensitivity in detecting tubercle bacilli in FNA specimens.
“Ziehl Nelson (ZN) smear microscopy has low sensitivity in the detection of tubercle bacilli in FNA specimens [7,8].”
From Diagnostic accuracy of Xpert MTB/RIF assay and non-molecular methods for the diagnosis of tuberculosis lymphadenitis (2019) · cites this paper as [8]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
No references could be resolved yet.
Research works printed but not linked: 19
- [1]1. Lucas PF. Lymphnode smears in diagnosis of lymphadenopathy. Blood 1963;10:1030-54.
- [2]2. Finfer M, Perchik A, Burstein ED. Fine needle aspiration biopsy diagnosis of tuberculous lymphadenitis in patients with and with out the Acquired Immunodefi ciency Syndrome. Acta Cytol, 1991;35(3):325-32.
- [3]3. Prasad RR, Narasimhan R, Sankaran V, Veliath AJ. Fine needle aspiration cytology in the diagnosis of superficial lymphadenopathy, an analysis of 2418 cases, Diagn Cytopathol, 1996;15(5):382-6.
- [4]4. Das DK, Pant JN, Chachra Kl, et al: Tuberculous lymphadenitis: Correlation of cellular components and necrosis in lymphnode aspirate with AFB positivity and bacillary count. Indian J Pathol Microbiol, 1990;33:110.
- [5]5. Rajwanshi A, Bhambhani S, Das DK. Fine 5. needle aspiration cytology diagnosis of tuberculosis. Diagn cytopathol, 1987;3:13-6.
- [7]7. Dlip K Das, Lymph nodes. In: Maruluce Bibbo eds. Comprehensive cytopathology 2nd ed. Philadelphia. W.B. Saunders. 1997;707-9.
- [8]8. Bailey TM, Akhtar M, Ali MA: Fine needle aspiration biopsy in the diagnosis of tuberculosis. Acta cytol 1985;29:732-6.
- [9]9. Das Dk, Bhambhani S, Pant JN, et al. Superficial and deep seated tuberculous lesions. Fine needle aspiration cytology diagnosis of 574 cases. Diagn cytopathol, 1992;8:211-5.
- [10]10. Gupta SK, Chungh TD, Sheikh ZA. Air - abah NAR: Cytodiagnosis of tuberculous lymphadenitis. A correlative study with microbiologic examination. Acta cytol, 1993;37:329-32.
- [11]11. Sadanah MM, Janyaram G: Acid Fast bacilli in aspiration smear from tuberculous lymphnodes an analysis of 255 cases. Acta Cytol, 1981;31:17-9.
- [12]12. Radhika S, Rajwanshi A, Kochhor S, et al. Abdominal tuberculosis: Diagnosis by Find needle aspiration cytology. Acta Cytol, 1993;37:673-8.
- [14]14. Qadri SMH, Akhar M, Ashraf M. Sensitivity of fine needle aspiration biopsy in the detection by Mycobacterial infections. Diagn cytopathol, 1991;7:142-6.
- [15]15. Nambuya A, Sewankambo N, Mugerwa J, et al. Tuberculous lymphadenitis associated with human immunodeficiency virus (HIV) in Uganda. J Clin Pathol, 1983;41:93-6.
- [16]16. Niedt GW, Schinella RA. Acquired Immunodeficency Syndrome: Clinicopathologic study of 56 Autopsies. Arch Pathol Lab Med, 1985;109:727-34.
- [17]17. G.Di Perri, Cazzodori A, Vento S, et al. Comparative histopathologic study of pulmonary tuberculosis in HIV infected and non infected patients Tubercle and lung disease, 1996;3:244.
- [18]18. Maygarden SJ, Flanders EL. Mycobacteria can be seen as "negative images" in cytology smears from patients with AIDS. Mod Pathol, 1989;2:239-42.
- [19]19. Krishnaswami H, Job CK. The role of Ziehl Neelsen and Fluorescent stains in tissue sections in the diagnosis of tuberculosis. Indian J Tuberc, 1974;21:18-21.
- [20]20. Rickman TW, Moyer NP. Increased sensitivity of acid fast smears. J clin Microbiol, 1980;11:618-24.
- [21]21. Kim SS, Chung SM, Kim JN,, Lee MA, Ha EH. J Korean Med Sci 1996:11(2):127-32.
Cited by
Sorted by citations
Fantahun, M., Kebede, A., Yenew, B., Gemechu, T., Mamuye, Y., Tadesse, M., Brhane, B., Jibriel, A., Solomon, D., & Yaregal, Z. (2019) · PLoS ONE · doi:10.1371/journal.pone.0222402 · Cited by 38 · see citing works
“Ziehl Nelson (ZN) smear microscopy has low sensitivity in the detection of tubercle bacilli in FNA specimens [7,8].”
BackgroundMethodTadele, A., Beyene, D., Hussein, J., Gemechu, T., Birhanu, A., Mustafa, T., Tsegaye, A., Aseffa, A., & Sviland, L. (2014) · BMC Infectious Diseases · doi:10.1186/s12879-014-0585-1 · Cited by 39 · see citing works
Mitra, S., Misra, R., & Rai, P. (2017) · Journal of Cytology · doi:10.4103/joc.joc_207_15 · Cited by 33 · see citing works
Tuberculosis Lymphadenitis in Ethiopia
Biadglegne, F., Tesfaye, W., Anagaw, B., Tessema, B., Debebe, T., Anagaw, B., Mulu, A., Sack, U., & Rodloff, A. C. (2013) · Japanese Journal of Infectious Diseases · doi:10.7883/yoken.66.263 · Cited by 32 · see citing works
Arul, P., Masilamani, S., & Akshatha, C. (2015) · Journal of Natural Science Biology and Medicine · doi:10.4103/0976-9668.166121 · Cited by 28 · see citing works
“Ergete and Bekele [20] 1997 7 (3.4) 131 (63.7) 67 (32.7) 71.7 Nidhi et al. [15] 2010 25 (14.3) 29 (16.4) 69 (39.2) 71 Chand et al. [16] 2013 156 (28.4) 120 (21.8) 85 (15.4) 44.54 Hemalatha et al .”
InfluentialP.G Resident, D. O. P. M., Singh, D. K. G., Tandon, D. S., Professor and Head, D. O. P. M., Nagdeote, D. S. T., Professor, D. O. P. M., Sharma, D. K., Assistant Professor, D. O. P. M., Kumar, D. A., & P.G Resident, D. O. P. M. (2017) · International Journal of Medical Research and Review · doi:10.17511/ijmrr.2017.i03.05 · Cited by 18 · see citing works
Khan, K. (2024) · Indian Journal of Tuberculosis · doi:10.1016/j.ijtb.2023.05.006 · Cited by 1 · see citing works
Das, S., Bandyopadhyay, U., & Nag, D. (2020) · International Journal of Current Research and Review · doi:10.31782/ijcrr.2020.121618 · Cited by 1 · see citing works
S, S., S, A., Munesh, M., & S, M. (2021) · International journal of scientific research · doi:10.36106/ijsr/5928314
- Authors
- Ergete, W., & Bekele, A.
- ORCID
- Bekele, Alemayehu
- Venue
- Ethiopian Journal of Health Development
- Publisher
- African Journals Online (AJOL)
- Published
- 2000-01-01
- Volume
- 14
- Issue
- 1
- ISSN
- 1021-6790 (ISSN-L)
- OpenAlex
- W2003918220
- Cited by
- 38
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 5 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 6 · 2016: 4 · 2015: 4 · 2014: 1 · 2013: 2
- FWCI
- 0.37
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Ethiopian Journal of Health Development (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Diagnosis and treatment of tuberculosis
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Surgery › Lymphadenopathy Diagnosis and Analysis
- Keywords
- acid-fast bacilli, fine needle aspiration cytology, extrapulmonary tuberculosis, tuberculous lymphadenitis, caseous necrosis, epithelioid granulomas, cell morphology, Ziehl-Neelsen staining, May-Grünwald-Giemsa staining, tuberculosis diagnosis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
