The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
The measurement of microbial carbohydrate exopolymers from intertidal sediments
Open accessAbstract
Measurements of microbial extracellular polymeric substances (EPS) of natural field populations are required to understand the processes of biogenic stabilization and the microbial ecology of intertidal sediments. EPS in sediments can be measured by the phenol‐sulfuric acid assay to measure carbohydrate concentration in sediment samples. We conducted comparative studies of storage and extraction methods for carbohydrate fractions on intertidal estuarine sediments. Measurements of both total and colloidal (material remaining in suspension after aqueous extraction and centrifugation) carbohydrate concentrations were highly dependent on storage conditions, sample size, extraction media, and time. Precipitation (in 70% ethanol) of EPS from saline (25‰) extractions of colloidal carbohydrate (colloidal S) from four different sediment microbial assemblages showed that 20% of colloidal carbohydrate present in extracts consisted of EPS. Concentrations of colloidal S were highly correlated with diatom biomass as determined by chlorophyll a concentrations. Extractions that used 100 mM Na 2 EDTA as the extracting medium (colloidal EDTA) increased the concentrations of colloidal carbohydrate obtained and, in sediments dominated by bacteria and cyanobacteria, increased the percentage of EPS in the material to 38%. Uronic acids comprised 20% of the total amount of carbohydrate measured in both total sediment and colloidal‐S extracts but increased to 65% in colloidal‐EDTA extracts, suggesting that colloidal‐EDTA extractions remove bacterial capsular EPS and EPS more closely associated with sediment particles.
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Cites
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
Use of nuclepore filters for counting bacteria by fluorescence microscopy
Hobbie, J. E., Daley, R. J., & Jasper, S. (1977) · Applied and Environmental Microbiology · doi:10.1128/aem.33.5.1225-1228.1977 · Cited by 4,244
Decho, A. W. (1990) · Oceanography and Marine Biology/Oceanography and marine biology - an annual review · Cited by 1,199
DIATOM EXTRACELLULAR POLYMERIC SUBSTANCES: FUNCTION, FINE STRUCTURE, CHEMISTRY, AND PHYSIOLOGY
Hoagland, K. D., Rosowski, J. R., Gretz, M. R., & Roemer, S. C. (1993) · Journal of Phycology · doi:10.1111/j.0022-3646.1993.00537.x · Cited by 675
Paterson, D. M. (1989) · Limnology and Oceanography · doi:10.4319/lo.1989.34.1.0223 · Cited by 456
Self-citationBiofilms and Microbial Fouling
Characklis, W. G., & Cooksey, K. E. (1983) · Advances in Applied Microbiology · doi:10.1016/s0065-2164(08)70355-1 · Cited by 348
Comparison of Bacterial Extracellular Polymer Extraction Methods
Brown, M. J., & Lester, J. N. (1980) · Applied and Environmental Microbiology · doi:10.1128/aem.40.2.179-185.1980 · Cited by 295
Yallop, M. L., de Winder, B., Paterson, D. M., & Stal, L. J. (1994) · Estuarine, Coastal and Shelf Science · doi:10.1016/s0272-7714(06)80010-7 · Cited by 294
Self-citationThe role of extracellular polysaccharides in biofilms
Christensen, B. E. (1989) · Journal of Biotechnology · doi:10.1016/0168-1656(89)90064-3 · Cited by 211
Effects of bacterial exopolymer adhesion on the entrainment of sand
Dade, W. B., Davis, J. D., Nichols, P. D., Nowell, A. R. M., Thistle, D., Trexler, M. B., & White, D. C. (1990) · Geomicrobiology Journal · doi:10.1080/01490459009377874 · Cited by 185
Cited by
Microbial biofilms in intertidal systems: an overview
Decho, A. W. (2000) · Continental Shelf Research · doi:10.1016/s0278-4343(00)00022-4 · Cited by 894
Cheah, Y. T., & Chan, D. J. C. (2022) · Journal of Applied Microbiology · doi:10.1111/jam.15455 · Cited by 108
Metadata
- Authors
- Underwood, G. J. C., Paterson, D. M., & Parkes, R. J.
- Venue
- Limnology and Oceanography
- Publisher
- Wiley
- Published
- 1995-11
- Volume
- 40
- Issue
- 7
- Pages
- 1243-1253
- ISSN
- 0024-3590 (ISSN-L)
- 1939-5590
- OpenAlex
- W2167294269
- Cited by
- 367
- References
- 18
- FWCI
- 6.27
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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