Evaluation of the Importance of Centrifugation as a Component of Zinc Sulfate Fecal Flotation Examinations
Abstract
Fifty canine fecal samples were evaluated by five flotation procedures to compare the sensitivity of the zinc sulfate (ZnSO 4 ) centrifugation flotation test with ZnSO 4 flotation tests using benchtop incubation during the flotation period. One or more parasite species were detected in 40 samples. Results showed that centrifugation with ZnSO 4 solution was significantly more likely to detect a positive sample than benchtop procedures. The difference in procedures was due primarily to increased detection of Trichuris eggs and Giardia cysts by centrifugal flotation. No significant difference was seen in the ability of benchtop procedures to detect positive samples when tests sat either for 5 or 10 minutes before examination.
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The larger the amount of faeces examined and the longer the set-aside time, the higher the likelihood of detecting T. vulpis eggs.
“The larger the amount of faeces examined and the longer the set-aside time, the higher is the likelihood of detecting T. vulpis eggs [63,64].”
From Are we paying too much attention to cardio-pulmonary nematodes and neglecting old-fashioned worms like Trichuris vulpis? (2011) · cites this paper as [64]
Whipworm eggs have a specific gravity of 1.15, so a solution of more than 1.20-1.35 s.gr (e.g. zinc sulphate) should be selected to allow flotation.
“As whipworm eggs have 1.15 s. gr., then a solution of more than 1.20-1.35 s.gr (e.g. using zinc sulphate) should be selected to allow flotation”
From Are we paying too much attention to cardio-pulmonary nematodes and neglecting old-fashioned worms like Trichuris vulpis? (2011) · cites this paper as [64]
Centrifugation of stool samples before the flotation procedure may help in detection.
“centrifugation of stool samples before the flotation procedure may help in detection [63,64].”
From Are we paying too much attention to cardio-pulmonary nematodes and neglecting old-fashioned worms like Trichuris vulpis? (2011) · cites this paper as [64]
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- Authors
- Zajac, A. M., Johnson, J., & King, S. E.
- Affiliations
- Virginia–Maryland College of Veterinary Medicine (US)
- ORCID
- Zajac, Anne M.
- Venue
- Journal of the American Animal Hospital Association
- Publisher
- American Animal Hospital Association
- Published
- 2002-05
- Volume
- 38
- Issue
- 3
- Pages
- 221-224
- ISSN
- 0587-2871 (ISSN-L)
- 1547-3317
- OpenAlex
- W2173153644
- Cited by
- 98
- References
- 7
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 3 · 2023: 5 · 2022: 4 · 2021: 4 · 2020: 2 · 2019: 8 · 2018: 4 · 2017: 4 · 2016: 3 · 2015: 1 · 2014: 9 · 2013: 6 · 2012: 1
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Animal Hospital Association (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Parasitic infections in humans and animals
- Keywords
- fecal flotation, canine parasites, Giardia cysts, parasite detection sensitivity, dogs
- MeSH terms
- Ancylostoma, Animals, Centrifugation, Dog Diseases, Dogs, Feces, Giardia, Giardiasis, Intestinal Diseases, Parasitic, Parasite Egg Count, Predictive Value of Tests, Sensitivity and Specificity, Toxocara, Trichuris, Zinc Sulfate
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