Peptides-derived from Scales of Branchiostegus japonicus Inhibit Ultraviolet B-induced Oxidative Damage and Photo-aging in Skin Cells
Open accessAbstract
생체에서 가장 많은 비율로 분포하고 있는 콜라겐 펩타이드는 동물의 뼈와 해양 생물의 비늘에 많이 함유되어 있다. 콜라겐은 동물 생체의 여러 결합조직에서 구조 단백질로 흔하게 발견된다. 또한, 이들은 생물 의료 자재, 제약, 화장품, 식품 및 가죽 산업에 널리 이용된다. 각종 어류 비늘에서 추출된 펩타이드는 UVB 조사에 의해 유도된 피부의 손상 및 광 노화에 대한 보호 효과가 있었다. 그럼에도 불구하고, UVB 조사에 대한 옥돔 비늘 유래의 펩타이드 특성은 명확히 알려져 있지 않다. 이 연구에서는 옥돔 비늘 추출물에서 분리된 1 kDa 이상(HMP)과 1 kDa 이하(LMP)의 펩타이드를 이용하여 UVB 조사에 의해 유도된 피부 손상과 광 노화에 대한 효과를 연구하였다. 이들 펩타이드는 농도 의존적으로 DPPH 라디칼 소거능을 보였으며 LMP는 HaCaT 인간 피부세포에서 UVB 조사에 의해 유도된 세포 지질 과산화 산물인 8-isoprostane 생성을 억제하였다. 그리고 LMP와 HMP는 B16F10 마우스 흑색종 세포에서 tyrosinase 활성 및 melanin 함량을 감소시켰으며 또한 HaCaT 세포에서 UVB로 유도된 elastase 활성을 감소시켰고 matrix metalloproteinase-1의 활성을 감소시켰다. 이러한 결과는 옥돔 비늘에서 유래된 펩타이드가 미백효과, 항산화제 및 광 노화 억제제로서 유용한 물질이 될 것으로 기대된다. Collagen peptides, which are found at high concentrations in the human body, are present in animal bones and the skin of marine organisms, namely, fish scales. Collagen is the most abundant structural protein of various connective tissues in animals. Furthermore, it is widely used in biomedical material, pharmaceutical, cosmetic, food, and leather industries. Peptides extracted from scales of various fish protect against ultraviolet B (UVB)-induced skin damage and photo-aging. However, the protective effects of collagen peptides derived from the scales of Branchiostegus japonicus against UVB exposure are unclear. This study investigated the effects of peptides larger than 1 kDa (high-molecular weight peptides [HMP]) and smaller than 1 kDa (low-molecular weight peptides [LMP]), derived from extracts of B. japonicus scales, against UVB-induced skin damage and photo-aging. These peptides scavenged 1,1-diphenyl-2-picrylhydrazyl radicals in a dose-dependent manner. In UVB-exposed HaCaT human keratinocytes, LMP inhibited 8-isoprostane generation, a marker of cellular lipid peroxidation. The peptides also suppressed the UVB-induced increase in tyrosinase activity and melanin content in B16F10 mouse melanoma cells. In addition, the LMP and HMP treatment suppressed UVB-induced elastase and matrix metalloproteinase-1 activities in the HaCaT cells. These results indicate that peptides derived from B. japonicus scales have antioxidant, antiphoto-aging, and skin-whitening effects.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Oh et al.'s study lacks a control group or comparison with existing treatments.
“Additionally, the study lacks a control group or comparison with existing treatments, which would provide a better understanding of the efficacy of the peptides.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [49]
The Results compare peptide-treated cells with untreated and UVB-only control groups and report a positive-control group treated with NAC. The methods also describe groups treated with NAC and arbutin alongside the peptides. Thus, the study did include controls and comparator treatments, contrary to the claim. (LLM, conf=0.91)
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredOh et al.'s study on peptides from Branchiostegus japonicus has limited evidence because sample size and experimental design are not clearly described.
“However, the level of evidence provided in this study is limited. The sample size and experimental design are not clearly described, making it difficult to assess the validity and generalizability of the findings.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [49]
The paper’s experimental and statistical procedures (section labeled 고 찰; page unavailable in our copy) describe cell seeding, peptide and UVB treatments, controls, assays, and at least three repetitions of each experiment. This contradicts the claim that its sample size and experimental design are not clearly described. The broader judgment about validity and generalizability is the citing authors’ assessment, not a limitation the source states. (LLM, conf=0.88)
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredOh et al.'s results show promising effects of peptides in reducing oxidative damage and photoaging.
“While the results of the study show promising effects of the peptides in reducing oxidative damage and photoaging, further research with a larger sample size, randomized controlled trials, and longer follow‐up periods are needed to confirm these findings.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [49]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Carmichael, J., DeGraff, W., Gazdar, A. F., Minna, J. D., & Mitchell, J. B. (1987) · PubMed · Cited by 3,813 · see citing works
Decreased Collagen Production in Chronologically Aged Skin
Varani, J., Dame, M. K., Rittie, L., Fligiel, S. E. G., Kang, S., Fisher, G. J., & Voorhees, J. J. (2006) · The American Journal of Pathology · doi:10.2353/ajpath.2006.051302 · Cited by 910 · see citing works
Enzymatic control of pigmentation in mammals
Hearing, V. J., & Tsukamoto, K. (1991) · The FASEB Journal · doi:10.1096/fasebj.5.14.1752358 · Cited by 754 · see citing works
Collagenase unwinds triple‐helical collagen prior to peptide bond hydrolysis
Chung, L., Dinakarpandian, D., Yoshida, N., Lauer‐Fields, J. L., Fields, G. B., Visse, R., & Nagase, H. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600318 · Cited by 677 · see citing works
Isolation and characterization of fish scale collagen of higher thermal stability
Pati, F., Adhikari, B., & Dhara, S. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2009.12.133 · Cited by 406 · see citing works
Hosoi, J., Abe, E., Suda, T., & Kuroki, T. (1985) · PubMed · Cited by 295 · see citing works
Ogawa, M., Portier, R. J., Moody, M. W., Bell, J., Schexnayder, M. A., & Losso, J. N. (2004) · Food Chemistry · doi:10.1016/j.foodchem.2004.02.006 · Cited by 263 · see citing works
Lipid rafts: structure, function and role in HIV, Alzheimer's and prion diseases
Fantini, J., Garmy, N., Mahfoud, R., & Yahi, N. (2002) · Expert Reviews in Molecular Medicine · doi:10.1017/s1462399402005392 · Cited by 258 · see citing works
Mammalian tyrosinase. A comparison of tyrosine hydroxylation and melanin formation
Hearing, V. J., & Ekel, T. M. (1976) · Biochemical Journal · doi:10.1042/bj1570549 · Cited by 209 · see citing works
Tsuji, N., Moriwaki, S., Suzuki, Y., Takema, Y., & Imokawa, G. (2001) · Photochemistry and Photobiology · doi:10.1562/0031-8655(2001)0740283troesb2.0.co2 · Cited by 195 · see citing works
Effects of Collagen Peptide Ingestion on UV-B-Induced Skin Damage
TANAKA, M., KOYAMA, Y.-I., & NOMURA, Y. (2009) · Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.80649 · Cited by 174 · see citing works
Matsumura, Y., & Ananthaswamy, H. N. (2002) · Expert Reviews in Molecular Medicine · doi:10.1017/s146239940200532x · Cited by 163 · see citing works
Sugimoto, K., Nishimura, T., Nomura, K., Sugimoto, K., & Kuriki, T. (2004) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.27.510 · Cited by 154 · see citing works
Plant physiological responses to UV-B radiation
Zlatev, Z. S., Lidon, F. C., & Kaimakanova, M. (2012) · Emirates Journal of Food and Agriculture · doi:10.9755/ejfa.v24i6.14669 · Cited by 144 · see citing works
Barker, D. L. A., Dixon, K., Medrano, E. E., Smalara, D., Im, S., Mitchell, D., Babcock, G. F., & ABDEL‐MALEK, Z. A. (1995) · PubMed · Cited by 137 · see citing works
Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts
Moon, H. J., Lee, S. R., Shim, S. N., Jeong, S. H., Stonik, V. A., Rasskazov, V. A., Zvyagintseva, T., & Lee, Y. H. (2008) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.31.284 · Cited by 131 · see citing works
Sugimoto, K., Nishimura, T., Nomura, K., Sugimoto, K., & Kuriki, T. (2003) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.51.798 · Cited by 126 · see citing works
Nazeer, R. A., Kumar, N. S. S., & Ganesh, R. J. (2012) · Peptides · doi:10.1016/j.peptides.2012.03.028 · Cited by 124 · see citing works
Boissy, R. E., Sakai, C., Zhao, H., Kobayashi, T., & Hearing, V. J. (1998) · Experimental Dermatology · doi:10.1111/j.1600-0625.1998.tb00324.x · Cited by 121 · see citing works
Plant physiological responses to UV-B radiation
Zlatev, Z. S., Lidon, F. J. C., & Kaimakanova, M. (2012) · Emirates Journal of Food and Agriculture · doi:10.9755/ejfa.v24i6.481501 · Cited by 120 · see citing works
Preparation and partial characterization of collagen sheet from fish (Lates calcarifer) scales
Sankar, S., Sekar, S., Mohan, R., Rani, S., Sundaraseelan, J., & Sastry, T. P. (2007) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2007.08.003 · Cited by 115 · see citing works
4,4′-Dihydroxybiphenyl as a New Potent Tyrosinase Inhibitor
Kim, Y. J., No, J. K., Lee, J., & Chung, H. Y. (2005) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.28.323 · Cited by 104 · see citing works
Photoaging and topical tretinoin: therapy, pathogenesis, and prevention
Kang, S. (1997) · Archives of Dermatology · doi:10.1001/archderm.133.10.1280 · Cited by 95 · see citing works
Photoaging and Topical Tretinoin
Kang, S. (1997) · Archives of Dermatology · doi:10.1001/archderm.1997.03890460104012 · Cited by 95 · see citing works
Age dependent increase of elastase type protease activity in mouse skin
Labat-Robert, J., Fourtanier, A., Boyer-Lafargue, B., & Robert, L. (2000) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/s1011-1344(00)00085-3 · Cited by 90 · see citing works
Imokawa, G. (2007) · Archives of Dermatological Research · doi:10.1007/s00403-007-0798-x · Cited by 89 · see citing works
Pigmentation effects of solar‐simulated radiation as compared with UVA and UVB radiation
Wolber, R., Schlenz, K., Wakamatsu, K., Smuda, C., Nakanishi, Y., Hearing, V., & Ito, S. (2008) · Pigment Cell & Melanoma Research · doi:10.1111/j.1755-148x.2008.00470.x · Cited by 86 · see citing works
Review of Major Theories of Skin Aging
Gragnani, A., Cornick, S. M., Chominski, V., de Noronha, S. M. R., de Noronha, S. A. A. C., & Ferreira, L. M. (2014) · Advances in Aging Research · doi:10.4236/aar.2014.34036 · Cited by 75 · see citing works
Oxidative damage, skin aging, antioxidants and a novel antioxidant rating system.
Palmer, D. M., & Kitchin, J. S. (2010) · PubMed · Cited by 65 · see citing works
Puglia, C., Offerta, A., Saija, A., Trombetta, D., & Cardile, V. (2014) · Journal of Cosmetic Dermatology · doi:10.1111/jocd.12083 · Cited by 57 · see citing works
Akiu, S., Suzuki, Y., Asahara, T., et al. (1991) · PubMed · Cited by 54 · see citing works
Viator, R. J., Rest, R. F., Hildebrandt, E. F., & McGee, D. J. (2008) · BMC Biochemistry · doi:10.1186/1471-2091-9-15 · Cited by 48 · see citing works
Tada, M., Kohno, M., & Niwano, Y. (2014) · BMC Biochemistry · doi:10.1186/1471-2091-15-23 · Cited by 34 · see citing works
Hepatoprotective and Anticancer Activities of Allomyrina dichotoma Larvae
Lee, J., Jo, D., Lee, A., et al. (2015) · Journal of Life Science · doi:10.5352/jls.2015.25.3.307 · Cited by 27 · see citing works
RALGA (Diacnéal®) Decreases Melanin Content in a Human Skin Model
Boisnic, S., Branchet, M., Nocera, T., et al. (2005) · Dermatology · doi:10.1159/000081503 · Cited by 9 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Cosmeceuticals in photoaging: A review.
Chan, L. K. W., Lee, K. W. A., Lee, C. H., Lam, K. W. P., Lee, K. F. V., Wu, R., Wan, J., Shivananjappa, S., Sky, W. T. H., Choi, H., et al. (2024) · Skin Research and Technology · doi:10.1111/srt.13730 · Cited by 23 · see citing works
- Authors
- Oh, M. C., Kim, K. C., Ko, C.-I., Ahn, Y. S., & Hyun, J. W.
- Affiliations
- Jeju National University (KR)
- Corresponding author
- Hyun, Jin Won
- Venue
- Journal of Life Science
- Publisher
- Korean Society of Life Science
- Published
- 2015-03-30
- Volume
- 25
- Issue
- 3
- Pages
- 269-275
- ISSN
- 1225-9918 (ISSN-L)
- OpenAlex
- W1491263751
- Cited by
- 7
- References
- 35
- Citations by year
- 2024: 2 · 2023: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2016: 1
- FWCI
- 0.74
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Journal of Life Science (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- Branchiostegus japonicus, fish scales, collagen peptides, photoaging, skin aging, antioxidant activity, DPPH radical scavenging, 8-isoprostane, lipid peroxidation, tyrosinase inhibition, melanin, elastase, MMP-1, HaCaT keratinocytes, B16F10 melanoma cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
