Natural Lactic Acid Bacteria Population and Silage Fermentation of Whole-crop Wheat
Open accessAbstract
Winter wheat is a suitable crop to be ensiled for animal feed and China has the largest planting area of this crop in the world. During the ensiling process, lactic acid bacteria (LAB) play the most important role in the fermentation. We investigated the natural population of LAB in whole-crop wheat (WCW) and examined the quality of whole-crop wheat silage (WCWS) with and without LAB inoculants. Two Lactobacillus plantarum subsp. plantarum strains, Zhengzhou University 1 (ZZU 1) selected from corn and forage and grass 1 (FG 1) from a commercial inoculant, were used as additives. The silages inoculated with LAB strains (ZZU 1 and FG 1) were better preserved than the control, with lower pH values (3.5 and 3.6, respectively) (p<0.05) and higher contents of lactic acid (37.5 and 34.0 g/kg of fresh matter (FM), respectively) (p<0.05) than the control. Sixty LAB strains were isolated from fresh material and WCWS without any LAB inoculation. These LAB strains were divided into the following four genera and six species based on their phenotypic, biochemical and phylogenetic characteristics: Leuconostoc pseudomesenteroides, Leuconostoc citreum, Weissella cibaria, Lactococcus lactis subsp. lactis, Lactobacillus buchneri, and Lactobacillus plantarum subsp. plantarum. However, the prevalent LAB, which was predominantly heterofermentative (66.7%), consisted of Leuconostoc pseudomesenteroides, Leuconostoc citreum, Weissella cibaria, and Lactobacillus buchneri. This study revealed that most of isolated LAB strains from control WCWS were heterofermentative and could not grow well at low pH condition; the selective inoculants of Lactobacillus strains, especially ZZU 1, could improve WCWS quality significantly.
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Lactobacillus is the main bacterial group that plays a major role in the silage process, and changes in the number and species of Lactobacillus are closely related to changes in the quality of silage materials.
“Lactobacillus is the main bacterial group that plays a major role in the silage process, and changes in the number and species of Lactobacillus are closely related to changes in the quality of silage materials [44].”
From Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover (2021) · cites this paper as [44]
The Discussion (page unavailable) says lactobacilli play a particularly important role in sustained lactic acid fermentation, and that lactobacilli counts and the species present can affect silage quality. But it identifies lactic acid bacteria as the predominant group, not Lactobacillus alone, and says species differences might influence quality rather than establishing the claimed close relationship between changes in Lactobacillus numbers and species and changes in quality. (LLM, conf=0.83)
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“Lactobacillus is the main bacterial group that plays a major role in the silage process, and changes in the number and species of Lactobacillus are closely related to changes in the quality of silage materials [44].”
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- Authors
- Ni, K., Wang, Y., Cai, Y., & Pang, H.
- Affiliations
- Institute of Livestock and Grassland Science (JP)
- Corresponding author
- Pang, Huili
- Venue
- Asian-Australasian Journal of Animal Sciences
- Published
- 2015-05-07
- Volume
- 28
- Issue
- 8
- Pages
- 1123-1132
- ISSN
- 1011-2367 (ISSN-L)
- 1976-5517
- PMC
- PMC4478480
- OpenAlex
- W2165278212
- Cited by
- 54
- References
- 25
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 4 · 2023: 3 · 2022: 7 · 2021: 8 · 2020: 7 · 2019: 7 · 2018: 3 · 2017: 4 · 2016: 1
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Asian-Australasian Journal of Animal Sciences (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- lactic acid bacteria, silage fermentation, silage inoculants, heterofermentative lactic acid bacteria, Leuconostoc pseudomesenteroides, Leuconostoc citreum, Weissella cibaria, Lactococcus lactis subsp. lactis, Lactobacillus buchneri, silage quality, silage pH, lactic acid content, winter wheat
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