Advantage of Using Inosine at the 3′ Termini of 16S rRNA Gene Universal Primers for the Study of Microbial Diversity
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To overcome the shortcomings of universal 16S rRNA gene primers 8F and 907R when studying the diversity of complex microbial communities, the 3′ termini of both primers were replaced with inosine. A comparison of the clone libraries derived using both primer sets showed seven bacterial phyla amplified by the altered primer set (8F-I/907R-I) whereas the original set amplified sequences belonging almost exclusively to Proteobacteria(95.8%). Sequences belonging to Firmicutes(42.6%) and Thermotogae(9.3%) were more abundant in a library obtained by using 8F-I/907R-I at a PCR annealing temperature of 54°C, while Proteobacteria sequences were more frequent (62.7%) in a library obtained at 50°C, somewhat resembling the result obtained using the original primer set. The increased diversity revealed by using primers 8F-I/907R-I confirms the usefulness of primers with inosine at the 3′ termini in studying the microbial diversity of environmental samples.
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Forage intended for silage usually contains a low number of lactic acid bacteria, so starter cultures of selected strains are necessary.
“The forage before ensiling intended for silages usually contains a low number of lactic acid bacteria (LAB), so it is necessary to apply starter cultures of selected strains.”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Ben-Dov et al. 2006
Natural populations of LAB on plant materials are often heterofermentative and low in number.
“Natural populations of LAB on plant materials are often heterofermentative and low in number (Ben-Dov et al. 2006).”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Ben-Dov et al. 2006
The composition and abundance of epiphytic bacterial microflora on ensiled plant raw material is insufficient to initiate lactic acid production by the LAB present.
“The composition and abundance of the epiphytic bacterial microflora on ensiled plant raw material is insufficient to initiate the production of lactic acid by the LAB present.”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Ben-Dov et al. 2006
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- Authors
- Ben-Dov, E., Shapiro, O. H., Siboni, N., & Kushmaro, A.
- Affiliations
- Department of Biotechnology Engineering
- Ben-Gurion University of the Negev (IL)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2006-11
- Volume
- 72
- Issue
- 11
- Pages
- 6902-6906
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1636166
- OpenAlex
- W2107352655
- Cited by
- 100
- References
- 31
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 6 · 2020: 5 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 7 · 2012: 8
- FWCI
- 7.23
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UTS ePRESS (University of Technology Sydney) (submittedVersion)
- Topics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Physical Sciences › Environmental Science › Ecology › Environmental DNA in Biodiversity Studies
- Keywords
- 16S rRNA gene, universal primers, microbial diversity, bacterial phyla, Proteobacteria, Firmicutes, Thermotogae, PCR annealing temperature, clone libraries, environmental microbial communities
- MeSH terms
- Bacteria, Industrial Waste, Inosine, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Sodium Chloride, Genetic Variation, Water Microbiology, Polymerase Chain Reaction, Sequence Analysis, DNA, Ecosystem, DNA Primers, Genes, rRNA
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