PSPH Mediates the Metastasis and Proliferation of Non-small Cell Lung Cancer through MAPK Signaling Pathways
Open accessAbstract
Growing evidence indicates that phosphoserine phosphatase (PSPH) is up-regulated and correlates with prognosis in multiple types of cancer. However, little is known about the roles of PSPH in NSCLC. Thus, the aim of the present study was to demonstrate the expression of PSPH in human NSCLC and reveal its biological functions and the underlying mechanisms. qRT-PCR, western blot and immunohistochemistry were used to assess the expression of NSCLC patient specimens and NSCLC cell lines. The functions of PSPH in migration and invasion were determined using trans-well and wound-healing assays. Cell proliferation capacity was performed by cell counting kit-8 (CCK-8), colony formation assays and cell cycle analysis. We demonstrated that PSPH was overexpressed in NSCLC specimens compared with the adjacent non-tumorous specimens, and high expression of PSPH was associated with clinical stage, metastasis and gender in NSCLC. Decreased expression of PSPH inhibited NSCLC cells migration, invasion and proliferation. Most importantly, further experiments demonstrated that PSPH might regulate NSCLC progress through MAPK signaling pathways. Lastly, immunohistochemistry (IHC) revealed that the PSPH expression level was positively correlated with the clinical stage in NSCLC patients. These results suggest that PSPH may act as a putative oncogene and a potential therapeutic target in NSCLC.
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Augmented PSPH level is correlated with prognosis in multiple cancers including cutaneous squamous cell carcinoma, breast cancer, non-small cell lung cancer, colorectal cancer, and hepatocellular carcinoma.
“Additionally, several studies reported that augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma [9], breast cancer [10], non-small cell lung cancer [11], colorectal cancer [12] and hepatocellular carcinoma [13].”
From Phosphoserine phosphatase as a prognostic biomarker in patients with gastric cancer and its potential association with immune cells (2022) · cites this paper as [11]
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- Authors
- Liao, L., Ge, M., Zhan, Q., Huang, R., Ji, X., Liang, X., & Zhou, X.
- Affiliations
- Fudan University (CN)
- Corresponding author
- Liang, Xiaohua
- Zhou, Xinli
- Venue
- International Journal of Biological Sciences
- Publisher
- Ivyspring International Publisher
- Published
- 2019
- Volume
- 15
- Issue
- 1
- Pages
- 183-194
- ISSN
- 1449-2288 (ISSN-L)
- PMC
- PMC6329917
- OpenAlex
- W2904922853
- Cited by
- 48
- References
- 26
- Citations by year
- 2026: 9 · 2025: 10 · 2024: 6 · 2023: 6 · 2022: 9 · 2021: 5 · 2020: 2 · 2019: 1
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- International Journal of Biological Sciences (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Lung Cancer Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mechanisms of cancer metastasis
- Health Sciences › Medicine › Pathology and Forensic Medicine › Cancer Mechanisms and Therapy
- Keywords
- phosphoserine phosphatase, non-small cell lung cancer, MAPK signaling, metastasis, cell proliferation, cell migration and invasion, oncogenes, therapeutic target, clinical stage
- MeSH terms
- Carcinoma, Non-Small-Cell Lung, Cell Movement, Female, Humans, Immunohistochemistry, Lung Neoplasms, Male, Middle Aged, Neoplasm Invasiveness, Phosphoric Monoester Hydrolases, MAP Kinase Signaling System, Cell Line, Tumor, Cell Proliferation
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