The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
rsEGFP2 enables fast RESOLFT nanoscopy of living cells
Open accessAbstract
The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25–250 times faster recordings.
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Citations
Cites
Bayesian-Based Iterative Method of Image Restoration*
Richardson, W. H. (1972) · Journal of the Optical Society of America · doi:10.1364/josa.62.000055 · Cited by 4,160
An iterative technique for the rectification of observed distributions
Lucy, L. B. (1974) · The Astronomical Journal · doi:10.1086/111605 · Cited by 3,866 · cited 1×
“To remove this background, we deconvolved the final image by Richardson–Lucy (Richardson, 1972; Lucy, 1974) restoration with a 10% diffraction-limited PSF added to the RESOLFT PSF, as detailed previously (Hofmann et al., 2005).”
MethodDiffraction-unlimited all-optical imaging and writing with a photochromic GFP
Grotjohann, T., Testa, I., Leutenegger, M., Bock, H., Urban, N. T., Lavoie-Cardinal, F., Willig, K. I., Eggeling, C., Jakobs, S., & Hell, S. W. (2011) · Nature · doi:10.1038/nature10497 · Cited by 501
Self-citationNanoscopy of living brain slices with low light levels
Testa, I., Urban, N. T., Jakobs, S., Eggeling, C., Willig, K. I., & Hell, S. W. (2012) · Neuron · doi:10.1016/j.neuron.2012.07.028 · Cited by 131
Self-citation
Cited by
Light-induced cell damage in live-cell super-resolution microscopy
Wäldchen, S., Lehmann, J., Klein, T., van de Linde, S., & Sauer, M. (2015) · Scientific Reports · doi:10.1038/srep15348 · Cited by 536
Metadata
- Authors
- Grotjohann, T., Testa, I., Reuss, M., Brakemann, T., Eggeling, C., Hell, S. W., & Jakobs, S.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2012-12-31
- Volume
- 1
- Pages
- e00248-e00248
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2098275652
- Cited by
- 229
- References
- 4
- FWCI
- 6.61
- Type
- journal article
- Language
- en
- Open access
- gold
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- Grotjohann, T., Testa, I., Reuss, M., Brakemann, T., Eggeling, C., Hell, S. W., & Jakobs, S. (2012). rsEGFP2 enables fast RESOLFT nanoscopy of living cells. eLife. https://doi.org/10.7554/elife.00248
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
