The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis
Open accessAbstract
Autophagy is a catabolic process for bulk degradation of cytosolic materials mediated by double-membraned autophagosomes. The membrane determinant to initiate the formation of autophagosomes remains elusive. Here, we establish a cell-free assay based on LC3 lipidation to define the organelle membrane supporting early autophagosome formation. In vitro LC3 lipidation requires energy and is subject to regulation by the pathways modulating autophagy in vivo. We developed a systematic membrane isolation scheme to identify the endoplasmic reticulum–Golgi intermediate compartment (ERGIC) as a primary membrane source both necessary and sufficient to trigger LC3 lipidation in vitro. Functional studies demonstrate that the ERGIC is required for autophagosome biogenesis in vivo. Moreover, we find that the ERGIC acts by recruiting the early autophagosome marker ATG14, a critical step for the generation of preautophagosomal membranes.
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Cites
AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1
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LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
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“We then monitored autophagosome biogenesis by immunofluorescence analysis of LC3 puncta formation (Klionsky et al., 2012).”
InfluentialSelf-citationThe role of autophagy during the early neonatal starvation period
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Mammalian autophagy: core molecular machinery and signaling regulation
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The Atg12-Atg5 Conjugate Has a Novel E3-like Activity for Protein Lipidation in Autophagy
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“Furthermore, cytosol deficient in ATG7, ATG3 or ATG5, key factors in the ubiquitin-like pathway for LC3 lipidation, failed to generate lipidated T7-LC3 in vitro (Figure 3D,E and F).”
Influential
Cited by
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Self-citationThe autophagosome: origins unknown, biogenesis complex
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Autophagy induction via STING trafficking is a primordial function of the cGAS pathway
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Autophagy and Neurodegeneration: Pathogenic Mechanisms and Therapeutic Opportunities
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Autophagy during viral infection — a double-edged sword
Choi, Y., Bowman, J. W., & Jung, J. U. (2018) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0003-6 · Cited by 838
Metadata
- Authors
- Ge, L., Melville, D., Zhang, M., & Schekman, R.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2013-08-06
- Volume
- 2
- Pages
- e00947-e00947
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2127767424
- Cited by
- 445
- References
- 20
- FWCI
- 23.13
- Type
- journal article
- Language
- en
- Open access
- gold
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- Ge, L., Melville, D., Zhang, M., & Schekman, R. (2013). The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis. eLife. https://doi.org/10.7554/elife.00947
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