The DOI matches its Crossref record; 80 of the 80 references it cites were found in a registry or an index.
Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity
Open accessAbstract
Nuclear pore complexes (NPCs) conduct massive transport mediated by shuttling nuclear transport receptors (NTRs), while keeping nuclear and cytoplasmic contents separated. The NPC barrier in Xenopus relies primarily on the intrinsically disordered FG domain of Nup98. We now observed that Nup98 FG domains of mammals, lancelets, insects, nematodes, fungi, plants, amoebas, ciliates, and excavates spontaneously and rapidly phase-separate from dilute (submicromolar) aqueous solutions into characteristic ‘FG particles’. This required neither sophisticated experimental conditions nor auxiliary eukaryotic factors. Instead, it occurred already during FG domain expression in bacteria. All Nup98 FG phases rejected inert macromolecules and yet allowed far larger NTR cargo complexes to rapidly enter. They even recapitulated the observations that large cargo-domains counteract NPC passage of NTR⋅cargo complexes, while cargo shielding and increased NTR⋅cargo surface-ratios override this inhibition. Their exquisite NPC-typical sorting selectivity and strong intrinsic assembly propensity suggest that Nup98 FG phases can form in authentic NPCs and indeed account for the permeability properties of the pore.
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Metadata
- Authors
- Schmidt, H. B., & Görlich, D.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2015-01-06
- Volume
- 4
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2024387549
- Cited by
- 351
- References
- 80
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- gold
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