The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila
Open accessAbstract
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.
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Cites
A GAL4-Driver Line Resource for Drosophila Neurobiology
Jenett, A., Rubin, G. M., Ngo, T.-T. B., Shepherd, D., Murphy, C., Dionne, H., Pfeiffer, B. D., Cavallaro, A., Hall, D., Jeter, J., et al. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.09.011 · Cited by 1,449
Wagh, D. A., Rasse, T. M., Asan, E., Hofbauer, A., Schwenkert, I., Dürrbeck, H., Buchner, S., Dabauvalle, M.-C., Schmidt, M., Qin, G., et al. (2006) · Neuron · doi:10.1016/j.neuron.2006.02.008 · Cited by 908
Bruchpilot Promotes Active Zone Assembly, Ca 2+ Channel Clustering, and Vesicle Release
Kittel, R. J., Wichmann, C., Rasse, T. M., Fouquet, W., Schmidt, M., Schmid, A., Wagh, D. A., Pawlu, C., Kellner, R. R., Willig, K. I., et al. (2006) · Science · doi:10.1126/science.1126308 · Cited by 772
Fluorescent fusion protein knockout mediated by anti-GFP nanobody
Caussinus, E., Kanca, O., & Affolter, M. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2180 · Cited by 510
Cited by
Liu, L., MacKenzie, K. R., Putluri, N., Maletić-Savatić, M., & Bellen, H. J. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.08.024 · Cited by 556
Self-citationA genome-wide resource for the analysis of protein localisation in Drosophila
Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., KJ, V. V., Krishnan, R., et al. (2016) · eLife · doi:10.7554/elife.12068 · Cited by 359
Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes
Diao, F., Ironfield, H., Luan, H., Diao, F., Shropshire, W. C., Ewer, J., Marr, E., Potter, C. J., Landgraf, M., & White, B. H. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.01.059 · Cited by 347
A genetic, genomic, and computational resource for exploring neural circuit function
Davis, F. P., Nern, A., Picard, S., Reiser, M. B., Rubin, G. M., Eddy, S. R., & Henry, G. L. (2020) · eLife · doi:10.7554/elife.50901 · Cited by 268
Hsieh, Y.-C., Guo, C., Yalamanchili, H. K., Abreha, M., Al-Ouran, R., Li, Y., Dammer, E. B., Lah, J. J., Levey, A. I., Bennett, D. A., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.08.104 · Cited by 196
Metadata
- Authors
- Nagarkar-Jaiswal, S., Lee, P.-T., Campbell, M. E., Chen, K., Anguiano-Zarate, S., Cantu Gutierrez, M., Busby, T., Lin, W.-W., He, Y., Schulze, K. L., Booth, B. W., Evans-Holm, M., Venken, K. J., Levis, R. W., Spradling, A. C., Hoskins, R. A., & Bellen, H. J.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2015-03-31
- Volume
- 4
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2012418668
- Cited by
- 412
- References
- 4
- FWCI
- 14.02
- Type
- journal article
- Language
- en
- Open access
- gold
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- Nagarkar-Jaiswal, S., Lee, P.-T., Campbell, M. E., Chen, K., Anguiano-Zarate, S., Cantu Gutierrez, M., Busby, T., Lin, W.-W., He, Y., Schulze, K. L., Booth, B. W., Evans-Holm, M., Venken, K. J., Levis, R. W., Spradling, A. C., Hoskins, R. A., & Bellen, H. J. (2015). A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila. eLife. https://doi.org/10.7554/elife.05338
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