Bruchpilot Promotes Active Zone Assembly, Ca 2+ Channel Clustering, and Vesicle Release
Open accessAbstract
The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.
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Active zones of invertebrate synapses are similar to central vertebrate synapses, except for specializations such as t bars in Drosophila that appear to function in recruiting vesicles to active zones.
“Active zones of invertebrate synapses are similar to central vertebrate synapses, except for specializations such as the t bars in Drosophila that similar to synaptic ribbons appear to function in recruiting vesicles to active zones (Kittel et al., 2006).”
From The Presynaptic Active Zone (2012) · cites this paper as Kittel et al., 2006
The bruchpilot deletion decreased neurotransmitter release only by ~30%.
“the bruchpilot deletion decreased neurotransmitter release only by ~30% (Kittel et al., 2006).”
From The Presynaptic Active Zone (2012) · cites this paper as Kittel et al., 2006
Cites
Altrock, W. D., tom Dieck, S., Sokolov, M., Meyer, A. C., Sigler, A., Brakebusch, C., Fässler, R., Richter, K., Boeckers, T. M., Potschka, H., et al. (2003) · Neuron · doi:10.1016/s0896-6273(03)00088-6 · Cited by 258 · see citing works
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Südhof, T. C. (2012) · Neuron · doi:10.1016/j.neuron.2012.06.012 · Cited by 1,062 · see citing works
Calcium Channel Regulation and Presynaptic Plasticity
Catterall, W. A., & Few, A. P. (2008) · Neuron · doi:10.1016/j.neuron.2008.09.005 · Cited by 647 · see citing works
Nanodomain coupling between Ca2+ channels and sensors of exocytosis at fast mammalian synapses
Eggermann, E., Bucurenciu, I., Goswami, S. P., & Jonas, P. (2012) · Nature Reviews Neuroscience · doi:10.1038/nrn3125 · Cited by 500 · see citing works
Live-cell imaging of dendritic spines by STED microscopy
Nägerl, U. V., Willig, K. I., Hein, B., Hell, S. W., & Bonhoeffer, T. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0810028105 · Cited by 412 · see citing works
Self-citationCardona, A., Saalfeld, S., Preibisch, S., Schmid, B., Cheng, A., Pulokas, J., Tomancak, P., & Hartenstein, V. (2010) · PLoS Biology · doi:10.1371/journal.pbio.1000502 · Cited by 372 · see citing works
Wolff, T., Iyer, N. A., & Rubin, G. M. (2015) · Journal of Comparative Neurology · doi:10.1002/cne.23705 · Cited by 286 · see citing works
Synaptic vesicle recycling: steps and principles
Rizzoli, S. O. (2014) · The EMBO Journal · doi:10.1002/embj.201386357 · Cited by 317 · see citing works
Phelps, J. S., Hildebrand, D. G. C., Graham, B. J., Kuan, A. T., Thomas, L. A., Nguyen, T. M., Buhmann, J., Azevedo, A. W., Sustar, A., Agrawal, S., et al. (2021) · Cell · doi:10.1016/j.cell.2020.12.013 · Cited by 265 · see citing works
SYD-2 Liprin-α organizes presynaptic active zone formation through ELKS
Dai, Y., Taru, H., Deken, S. L., Grill, B., Ackley, B., Nonet, M. L., & Jin, Y. (2006) · Nature Neuroscience · doi:10.1038/nn1808 · Cited by 218 · see citing works
Wolff, T., & Rubin, G. M. (2018) · Journal of Comparative Neurology · doi:10.1002/cne.24512 · Cited by 156 · see citing works
Gamma‐aminobutyric acid (GABA)‐mediated neural connections in the <i>Drosophila</i> antennal lobe
Okada, R., Awasaki, T., & Ito, K. (2009) · Journal of Comparative Neurology · doi:10.1002/cne.21971 · Cited by 146 · see citing works
Multiple neuropeptides in the <i>Drosophila</i> antennal lobe suggest complex modulatory circuits
Carlsson, M. A., Diesner, M., Schachtner, J., & Nässel, D. R. (2010) · Journal of Comparative Neurology · doi:10.1002/cne.22405 · Cited by 146 · see citing works
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking
Wang, S. S. H., Held, R. G., Wong, M. Y., Liu, C., Karakhanyan, A., & Kaeser, P. S. (2016) · Neuron · doi:10.1016/j.neuron.2016.07.005 · Cited by 143 · see citing works
Visualizing presynaptic function
Kavalali, E. T., & Jorgensen, E. M. (2014) · Nature Neuroscience · doi:10.1038/nn.3578 · Cited by 126 · see citing works
Assembly of the presynaptic active zone
Emperador-Melero, J., & Kaeser, P. S. (2020) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2020.03.008 · Cited by 109 · see citing works
GABAB Receptor Modulation of Feedforward Inhibition through Hippocampal Neurogliaform Cells
Price, C. J., Scott, R., Rusakov, D. A., & Capogna, M. (2008) · Journal of Neuroscience · doi:10.1523/jneurosci.4673-07.2008 · Cited by 107 · see citing works
The role of histone deacetylase 6 in synaptic plasticity and memory
Perry, S., Kiragasi, B., Dickman, D., & Ray, A. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.01.028 · Cited by 49 · see citing works
Presynaptic active zone density during development and synaptic plasticity
Clarke, G. L. (2012) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2012.00012 · Cited by 33 · see citing works
Emperador-Melero, J., Andersen, J. W., Metzbower, S. R., Levy, A. D., Dharmasri, P. A., de Nola, G., Blanpied, T. A., & Kaeser, P. S. (2024) · Nature Neuroscience · doi:10.1038/s41593-024-01720-5 · Cited by 27 · see citing works
- Authors
- Kittel, R. J., Wichmann, C., Rasse, T. M., Fouquet, W., Schmidt, M., Schmid, A., Wagh, D. A., Pawlu, C., Kellner, R. R., Willig, K. I., Hell, S. W., Buchner, E., Heckmann, M., & Sigrist, S. J.
- Affiliations
- University of Würzburg (DE)
- Corresponding author
- Kittel, Robert J.
- Wichmann, Carolin
- Rasse, Tobias M.
- Heckmann, Manfred
- Sigrist, Stephan J.
- ORCID
- Venue
- Science
- Published
- 2006-05-19
- Volume
- 312
- Issue
- 5776
- Pages
- 1051-1054
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2102548112
- Cited by
- 772
- References
- 1
- Citations by year
- 2026: 17 · 2025: 29 · 2024: 31 · 2023: 33 · 2022: 31 · 2021: 38 · 2020: 45 · 2019: 34 · 2018: 47 · 2017: 40 · 2016: 33 · 2015: 38 · 2014: 59 · 2013: 41 · 2012: 45
- FWCI
- 16.98
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- other-oa
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- OPUS (Augsburg University) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- Bruchpilot, neurotransmitter release, synaptic vesicle release, T-bar, neuromuscular synapse, short-term plasticity, Drosophila, coiled-coil proteins, STED microscopy
- MeSH terms
- Action Potentials, Animals, Drosophila, Female, Larva, Male, Models, Neurological, Mutation, Nerve Tissue Proteins, Synapses, Synaptic Vesicles, Calcium Channels, Presynaptic Terminals, Drosophila Proteins
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