The DOI matches its Crossref record; 83 of the 83 references it cites were found in a registry or an index.
Tet2 and Tet3 cooperate with B-lineage transcription factors to regulate DNA modification and chromatin accessibility
Open accessAbstract
Ten-eleven translocation (TET) enzymes oxidize 5-methylcytosine, facilitating DNA demethylation and generating new epigenetic marks. Here we show that concomitant loss of Tet2 and Tet3 in mice at early B cell stage blocked the pro- to pre-B cell transition in the bone marrow, decreased Irf4 expression and impaired the germline transcription and rearrangement of the Igκ locus. Tet2/3-deficient pro-B cells showed increased CpG methylation at the Igκ 3’ and distal enhancers that was mimicked by depletion of E2A or PU.1, as well as a global decrease in chromatin accessibility at enhancers. Importantly, re-expression of the Tet2 catalytic domain in Tet2/3-deficient B cells resulted in demethylation of the Igκ enhancers and restored their chromatin accessibility. Our data suggest that TET proteins and lineage-specific transcription factors cooperate to influence chromatin accessibility and Igκ enhancer function by modulating the modification status of DNA.
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“which can physically interact with the myeloid master transcription factor (TF; Supplementary Fig. S5K and S5L; ref. 34).”
From PU.1-Dependent Enhancer Inhibition Separates Tet2 -Deficient Hematopoiesis from Malignant Transformation (2022) · cites this paper as [34]
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“Tet2 binds and governs site-specific DNA demethylation upon being recruited to the chromatin by DNA-binding TF, such as RUNX1 (51) or PU.1 (34)”
From PU.1-Dependent Enhancer Inhibition Separates Tet2 -Deficient Hematopoiesis from Malignant Transformation (2022) · cites this paper as [34]
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Metadata
- Authors
- Lio, C.-W., Zhang, J., González-Avalos, E., Hogan, P. G., Chang, X., & Rao, A.
- Affiliations
- Division of Signaling and Gene Expression, San Diego, United States
- Shanghai Jiao Tong University (CN)
- University of California San Diego (US)
- Corresponding author
- Chang, Xing
- Rao, Anjana
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2016-11-21
- Volume
- 5
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC5142813
- OpenAlex
- W2552742463
- Cited by
- 170
- References
- 83
- Citations by year
- 2026: 9 · 2025: 11 · 2024: 14 · 2023: 14 · 2022: 25 · 2021: 22 · 2020: 24 · 2019: 22 · 2018: 21 · 2017: 8
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Cancer Research Institute (Irvington Postdoctoral Fellowship)
- Ministry of Science and Technology of the People's Republic of China (2014CB943600)
- National Institutes of Health (AI44432, CA151535)
- Leukemia and Lymphoma Society (6187-12)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- TET2, TET3, B cell development, IRF4, germline transcription, DNA demethylation, 5-methylcytosine oxidation, CpG methylation, E2A, PU.1, transcription factor cooperation, bone marrow
- MeSH terms
- Interferon Regulatory Factor-4, Animals, B-Lymphocytes, Cell Differentiation, Chromatin, DNA, DNA-Binding Proteins, Gene Expression Regulation, Proto-Oncogene Proteins, Transcription, Genetic, 5-Methylcytosine, Dioxygenases, Interferon Regulatory Factors, Mice, Gene Knockout Techniques
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