Anti stress activity of phytoecdysteroids isolated from aerial part of Silene claviformis
Open accessAbstract
Aims: The aim of present study was to isolate the phytoedysteroids from aerial part of Silene claviformis (Caryophyllaceae) and investigate their biological activity. Place and Duration of Study: The investigation were carried out during 2019 and 2020 at laboratory of the chemistry of glycosides and department of the pharmacological and toxicology of Institute of the Chemistry of Plant Substances AS RUz, Tashkent, Uzbekistan. Methodology: The phytoecdysteroids were isolated from aerial part of Silene claviformis using chromatographic methods. Thin-layer chromatography made on Silufol UV-254 and Merck plates, Fluka Analytical Germany, by spraying with alcohol solution of vanillin and heating for 1-2 minutes for 90-1000, UV lamp light at 254 nm and 365 nm. Their structures were confirmed by NMR and IR spectroscopy. Sum of phytoecdysteroids was administered at a dose of 10 mg/kg orally. The data obtained during the experiments were processed and analyzed by the method of variation statistics using the Student t-criterion. Results: Silene claviformis contains 2-deoxyecdysterone (1), polypodine B (2), 20-hydroxyecdysone (3), ecdysterone-20,22-acetalisovaleric aldehyde (4),integristeron A (5),cyasterone (6), ecdysterone-20,22-acetalisovalerian (7), 2-deoxy-α-ecdysone (8). The compounds 2 and 6 are reported for the first time from this genus. The biological activity (stress-protective effect) of the mentioned phytoecdysteroids studied for the first time. These compounds were tested on male mice for their biological activities and results showed that, the adrenal gland mass increased in relation to the adrenal gland mass of intact animals by 42.6%, they showed a significant decrease in the content of ascorbic acid and cholesterol by 56.5 and 49.1%, respectively. A significant decrease in glycogen content (by 30.1%) and a noticeable activation of lipid peroxidation processes were noted in the liver, as indicated by an increase of 69.2% in the content of malondialdehyde in the organ. Conclusion: This is the first study reporting an orally biological investigation for Silene claviformis. The sum of phytoecdysteroids showed potent stress-protective activity. The stress-protective effect of phytoecdysteroids was more pronounced in compared the eleutherococcus extract.
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In Silene claviformis, the sum of PEs exhibits potent stress-protective activity.
“In Silene claviformis, the sum of PEs exhibits potent stress-protective activity [201].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [201]
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“In Silene claviformis, the sum of PEs exhibits potent stress-protective activity [201].”
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“According to Ramazonov et al. (2020), phytoecdysteroids have beneficial effects on the adrenal gland, in particular a powerful protective activity against stress at the level of this gland.”
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Self-citationMetabolic and Hormonal Modulation by Phytoecdysteroids in Mammals
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Self-citation
- Authors
- Ramazonov, N., Yusupova, U., Egamova, F., & Syrov, V.
- Affiliations
- Yunusov Institute of the Chemistry of Plant Substances (UZ)
- Venue
- Chemical Science International Journal
- Publisher
- Sciencedomain International
- Published
- 2020-09-05
- Volume
- 29
- Issue
- 7
- Pages
- 9-17
- ISSN
- 2249-0205 (ISSN-L)
- 2456-706X
- OpenAlex
- W3083295656
- Cited by
- 7
- References
- 34
- Citations by year
- 2026: 2 · 2025: 2 · 2023: 1 · 2022: 1 · 2021: 1
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Chemical Science International Journal (publishedVersion)
- Chemical Science International Journal (publishedVersion, cc-by)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Medicinal Plants and Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- Caryophyllaceae, polypodine B, 20-hydroxyecdysone, cyasterone, stress-protective effect, adrenal mass, vitamin C, cholesterol, glycogen, lipid peroxidation, malondialdehyde
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