The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293 T Cell–Based Systems
Abstract
This unit details the applications of one of the more common retroviral packaging systems, based on the highly transfectable 293T cell. The packaging system employs the use of the Phoenix cell lines. Calcium phosphate‐mediated transfection is described for efficient introduction of retroviral vector plasmid DNA into the cells to generate high yields of virion‐containing supernatant. An alternate protocol describes a method for transfecting retroviruses that contain a vesicular stomatitis virus G (VSV G) protein. Such virions are said to be “pseudotyped” with VSV G glycoprotein. Support protocols provide a simple method for concentrating VSV‐G‐pseudotyped retroviruses, as well as methods for culturing, cryopreserving, thawing, and drug selecting the Phoenix packaging cell lines. Finally, several methods for transfecting adherent or suspension cells with retroviruses are described.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943 · cited 1×
“This allows an internal promoter to function without influence from the LTR. Figure 10.17C.2 shows a tetracycline-regulated con- Current Protocols in Immunology Supplement 31 10.17.25 Molecular Biology struct (Gossen and Bujard, 1992; Hofmann et al., 1996).”
BackgroundInfluentialIn Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996) · Science · doi:10.1126/science.272.5259.263 · Cited by 4,903
Research works printed but not linked: 39
- 10.1128/JVI.65.9.4985-4990.1991 · DOI · Paper page
- 10.1016/0092-8674(89)90134-7 · DOI · Paper page
- Andreadis S.T., 1997, Moloney Murine Leukemia Virus‐derived retroviral vectors decay intracellularly with a half‐life in the range of 5.5 to 7.5 hr, J. Virol., 71, 7541, 10.1128/jvi.71.10.7541-7548.1997 · DOI
- 10.1002/(SICI)1097-0290(19980420)58:2/3<272::AID-BIT23>3.0.CO;2-D · doi:10.1002/(sici)1097-0290(19980420)58:2/3<272::aid-bit23>3.0.co;2-d
- 10.1128/JVI.61.5.1639-1646.1987 · DOI · Paper page
- 10.1073/pnas.90.17.8033 · DOI · Paper page
- Chattopadhyay S.K., 1981, Genomes of murine leukemia viruses isolated from wild mice, J. Virol., 39, 777, 10.1128/jvi.39.3.777-791.1981 · doi:10.1128/jvi.39.3.777-791.1981
- J.M. Coffin S.H. Hughes H.E. Varmus 1997 Cold Spring Harbor Laboratory Press Cold Spring Harbor NY
- 10.1016/0006-291X(90)91425-R · doi:10.1016/0006-291x(90)91425-r
- 10.1073/pnas.90.8.3539 · DOI · Paper page
- 10.1128/MCB.7.1.379 · DOI · Paper page
- 10.1182/blood.V83.1.43.43 · DOI · Paper page
- 10.1016/0042-6822(73)90341-3 · DOI · Paper page
- 10.1099/0022-1317-36-1-59 · DOI · Paper page
- 10.1084/jem.178.4.1175 · DOI
- 10.1016/S1074-7613(00)80551-8 · DOI · Paper page
- 10.1073/pnas.93.11.5185 · DOI · Paper page
- 10.1073/pnas.91.15.7071 · DOI · Paper page
- 10.1016/S0092-8674(00)81630-X · DOI · Paper page
- 10.1089/hum.1996.7.12-1405 · DOI · Paper page
- 10.1089/hum.1994.5.1-19 · DOI · Paper page
- 10.1128/jvi.66.8.5110-5113.1992 · DOI · Paper page
- Miller D.G., 1992, Tunicamycin treatment of CHO cells abrogates multiple blocks to retrovirus infection, one of which is due to a secreted inhibitor, J. Virol., 66, 78, 10.1128/jvi.66.1.78-84.1992 · doi:10.1128/jvi.66.1.78-84.1992
- 10.1128/JVI.70.8.5564-5571.1996 · DOI · Paper page
- Miller A.D., 1997, Murine retroviruses use at least six different receptors for entry into Mus dunni cells, J. Virol., 71, 4531, 10.1128/jvi.71.6.4531-4535.1997 · doi:10.1128/jvi.71.6.4531-4535.1997
- 10.1073/pnas.91.1.78 · DOI · Paper page
- 10.1128/jvi.70.8.5701-5705.1996 · DOI · Paper page
- 10.1073/pnas.90.18.8392 · DOI · Paper page
- 10.1385/0-89603-484-4:41 · doi:10.1385/0-89603-484-4:41
- 10.1084/jem.185.9.1711 · DOI · Paper page
- Sladek T.L., 1990, Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry, J. Virol., 64, 3135, 10.1128/jvi.64.6.3135-3138.1990 · doi:10.1128/jvi.64.6.3135-3138.1990
- 10.1093/nar/23.4.628 · DOI · Paper page
- 10.1093/intimm/7.8.1301 · doi:10.1093/intimm/7.8.1301
- 10.1073/pnas.91.3.1168 · DOI · Paper page
- Wang H., 1991, Plasma membrane receptors for ecotropic murine retroviruses require a limiting accessory factor, J. Virol., 65, 6468, 10.1128/jvi.65.12.6468-6477.1991 · DOI
- 10.1038/352729a0 · DOI · Paper page
- 10.1016/S0091-679X(08)60600-7 · DOI · Paper page
- 10.1073/pnas.83.10.3194 · DOI · Paper page
- 10.1128/JVI.69.6.3929-3932.1995 · DOI
Cited by
cGAS surveillance of micronuclei links genome instability to innate immunity
Mackenzie, K. J., Carroll, P., Martin, C.-A., Murina, O., Fluteau, A., Simpson, D. J., Olova, N., Sutcliffe, H., Rainger, J. K., Leitch, A., et al. (2017) · Nature · doi:10.1038/nature23449 · Cited by 1,752
Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis
Brentnall, M., Rodriguez-Menocal, L., De Guevara, R. L., Cepero, E., & Boise, L. H. (2013) · BMC Cell Biology · doi:10.1186/1471-2121-14-32 · Cited by 1,147
Cyclin-dependent kinases regulate the antiproliferative function of Smads
Matsuura, I., Denissova, N. G., Wang, G., He, D., Long, J., & Liu, F. (2004) · Nature · doi:10.1038/nature02650 · Cited by 503
CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions
Zimmermann, M., Murina, O., Reijns, M. A. M., Agathanggelou, A., Challis, R., Tarnauskaitė, Ž., Muir, M., Fluteau, A., Aregger, M., McEwan, A., et al. (2018) · Nature · doi:10.1038/s41586-018-0291-z · Cited by 401
Silvis, M. R., Kreger, B. T., Lien, W.-H., Klezovitch, O., Rudakova, G. M., Camargo, F. D., Lantz, D. M., Seykora, J. T., & Vasioukhin, V. (2011) · Science Signaling · doi:10.1126/scisignal.2001823 · Cited by 342
Metadata
- Authors
- Swift, S., Lorens, J., Achacoso, P., & Nolan, G. P.
- Venue
- Current Protocols in Immunology
- Publisher
- Wiley
- Published
- 1999-06
- Volume
- 31
- Issue
- 1
- Pages
- Unit 10.17C-Unit 10.17C
- ISSN
- 1934-3671 (ISSN-L)
- 1934-368X
- OpenAlex
- W1516459565
- Cited by
- 313
- References
- 2
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293 T Cell–Based Systems. https://app.cochat.ai/papers/10.1002/0471142735.im1017cs31
- The paper
- Swift, S., Lorens, J., Achacoso, P., & Nolan, G. P. (1999). Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293 T Cell–Based Systems. Current Protocols in Immunology. https://doi.org/10.1002/0471142735.im1017cs31
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
