The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation
Open accessAbstract
In addition to their chemical properties and topographical features, the mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, which include cell attachment, proliferation, and differentiation. In this work, multilayer films made of poly(L‐lysine)/hyaluronan (PLL/HA) of controlled stiffness have been used to investigate the effects of the mechanical properties of thin films on skeletal muscle cell (C2C12 cells) differentiation. Prior to differentiation, the cells need adhere and proliferate in a growth medium. Stiff films (E0 > 320 kPa) promote the formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films are not favorable for cell anchoring, spreading, or proliferation. C2C12 cells were then switched to a low serum‐containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions are only moderately affected by the film stiffness, the morphology of the myotubes exhibit striking stiffness‐dependent differences. Soft films allow differentiation only for a few days and the myotubes are very short and thick. Cell clumping followed by aggregate detachment is observed after ∼2 to 4 d. On stiffer films, significantly more elongated and thinner myotubes are observed for up to ∼2 weeks. Myotube striation is also observed but only for the stiffer films. These results demonstrate that film stiffness strongly modulates adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Calcium's Role in Mechanotransduction during Muscle Development
Benavides Damm, T., & Egli, M. (2014) · Cellular Physiology and Biochemistry · doi:10.1159/000356667 · Cited by 14,502
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites
Decher, G. (1997) · Science · doi:10.1126/science.277.5330.1232 · Cited by 10,054
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382
Cell Movement Is Guided by the Rigidity of the Substrate
Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y.-L. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76279-5 · Cited by 3,289
Cell locomotion and focal adhesions are regulated by substrate flexibility
Pelham, R. J., & Wang, Y.-L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13661 · Cited by 3,027
Exploring and Engineering the Cell Surface Interface
Stevens, M. M., & George, J. H. (2005) · Science · doi:10.1126/science.1106587 · Cited by 2,607
RGD modified polymers: biomaterials for stimulated cell adhesion and beyond
Hersel, U., Dahmen, C., & Kessler, H. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00343-0 · Cited by 2,582
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Myotubes differentiate optimally on substrates with tissue-like stiffness
Engler, A. J., Griffin, M. A., Sen, S., Bönnemann, C. G., Sweeney, H. L., & Discher, D. E. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200405004 · Cited by 1,743
Cited by
Influence of substrate stiffness on the phenotype of heart cells
Bhana, B., Iyer, R. K., Chen, W. L. K., Zhao, R., Sider, K. L., Likhitpanichkul, M., Simmons, C. A., & Radisic, M. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22647 · Cited by 373
Skeletal muscle tissue engineering: methods to form skeletal myotubes and their applications
Ostrovidov, S., Hosseini, V., Ahadian, S., Fujie, T., Parthiban, S. P., Ramalingam, M., Bae, H., Kaji, H., & Khademhosseini, A. (2014) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2013.0534 · Cited by 302
Aligned electrospun polymer fibres for skeletal muscle regeneration
School of Materials, B. G., Aviss, K., Gough, J., & Downes, S. (2010) · European Cells and Materials · doi:10.22203/ecm.v019a19 · Cited by 253
Cellular modulation by the elasticity of biomaterials
Han, F., Zhu, C., Guo, Q., Yang, H., & Li, B. (2016) · Journal of Materials Chemistry B · doi:10.1039/c5tb02077h · Cited by 83
Fujie, T., Ricotti, L., Desii, A., Menciassi, A., Dario, P., & Mattoli, V. (2011) · Langmuir · doi:10.1021/la203140a · Cited by 52
Metadata
- Authors
- Ren, K., Crouzier, T., Roy, C., & Picart, C.
- Venue
- Advanced Functional Materials
- Publisher
- Wiley
- Published
- 2008-05-09
- Volume
- 18
- Issue
- 9
- Pages
- 1378-1389
- ISSN
- 1616-301X (ISSN-L)
- 1616-3028
- OpenAlex
- W1999666701
- Cited by
- 273
- References
- 63
- FWCI
- 9.07
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation. https://app.cochat.ai/papers/10.1002/adfm.200701297
- The paper
- Ren, K., Crouzier, T., Roy, C., & Picart, C. (2008). Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation. Advanced Functional Materials. https://doi.org/10.1002/adfm.200701297
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
