Cited by 36Atherosclerosis and Cardiovascular DiseasesPluripotent Stem Cells Research3D Printing in Biomedical Research

In Vitro Modeling of Atherosclerosis Using iPSC‐Derived Blood Vessel Organoids

Kong, Dasom, Ryu, Jae‐Chul, Shin, Nari, Lee, Seung‐Eun, Kim, Nam Gyo, Kim, Hee‐Yeong, Kim, Min‐Ji, Choi, Jungju +2 more·2025·Advanced Healthcare Materials

Abstract

As modeling of atherosclerosis requires recapitulating complex interactions with vasculature and immune cells, previous in vitro models have limitations due to their insufficient 3D vascular structures. However, induced pluripotent stem cell-derived blood vessel organoids (BVOs) are applicable for modeling vascular diseases, containing multiple cell types, including endothelial and vascular smooth muscle cells self-assembled into a blood vessel structure. Atherosclerotic BVOs with a microenvironment associated with atherogenesis, such as shear stress, low-density lipoprotein, pro-inflammatory cytokine, and monocyte co-culture are successfully developed. In atherosclerotic BVOs, representative atherosclerotic phenotypes, including endothelial dysfunction, inflammatory responses, formation of foam cells and fibrous plaque, and moreover, calcification of the plaques are observed. To verify the drug response in this model, it is treated with clinically used lovastatin and confirm phenotype attenuation. Furthermore, the therapeutic efficacy of nano-sized graphene oxides (NGOs) is evaluated on atherosclerosis. Due to their anti-inflammatory effects, NGOs effectively alleviate the pathologic lesions in atherosclerotic BVOs by promoting macrophage polarization toward M2. These results suggest that atherosclerotic BVOs are advanced in vitro models suitable for drug discovery and elucidation of therapeutic mechanisms. From the perspective of precision medicine, this platform using pati…

Integrity signals

Registry

Crossref · 10.1002/adhm.202400919

Cites

83 of 84 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

3 of the 87 references couldn’t be matched to a known paper.

Research works printed but not linked: 6

  1. Hakan S., 2018, Atherosclerosis
  2. Islam K., 2016, SM J Biomed Eng., 2, 1008
  3. Chen J., 2022, Frontiers in Cardiovascular Medicine, 8, 2176
  4. Yvan‐Charvet L., 2007, J. Clin. Invest., 117, 3900
  5. Masiello T., 2018, Cells, 7
  6. Fatkhullina A. R., 2016, Biochemistry, 81, 1358

Cited by

Sorted by citations

Authors
Kong, D., Ryu, J., Shin, N., Lee, S., Kim, N. G., Kim, H., Kim, M., Choi, J., Kim, D., & Kang, K.
Affiliations
  • Seoul National University (KR)
  • Adult Stem Cell Research Center and Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University Seoul 08826 Republic of Korea
  • Sungshin Women's University (KR)
Corresponding author
  • Kim, Da‐Hyun
  • Kang, Kyung‐Sun
Venue
Advanced Healthcare Materials
Publisher
Wiley
Published
2024-11-24
Volume
14
Issue
1
Pages
e2400919-e2400919
ISSN
  • 2192-2640 (ISSN-L)
  • 2192-2659
OpenAlex
W4404656966
Cited by
36
References
87
Citations by year
2026: 25 · 2025: 11
FWCI
5.98
Type
journal article
Language
en
Open access
closed
Locations
  • Advanced Healthcare Materials (publishedVersion)
  • PubMed (publishedVersion)
Funders
Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
atherosclerosis, blood vessel organoids, induced pluripotent stem cells, endothelial dysfunction, foam cells, fibrous plaque, plaque calcification, low-density lipoprotein, shear stress, lovastatin, graphene oxide nanoparticles, macrophage polarization, M2 macrophages, anti-inflammatory therapy, drug screening, precision medicine
MeSH terms
Blood Vessels, Humans, Lovastatin, Organoids, Coculture Techniques, Atherosclerosis, Induced Pluripotent Stem Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review