Regulation of the expression of inducible nitric oxide synthase
Abstract
Nitric oxide (NO) generated by the inducible isoform of nitric oxide synthase (iNOS) is involved in complex immunomodulatory and antitumoral mechanisms and has been described to have multiple beneficial microbicidal, antiviral and antiparasital effects. However, dysfunctional induction of iNOS expression seems to be involved in the pathophysiology of several human diseases. Therefore iNOS has to be regulated very tightly. Modulation of expression, on both the transcriptional and post-transcriptional level, is the major regulation mechanism for iNOS. Pathways resulting in the induction of iNOS expression vary in different cells or species. Activation of the transcription factors NF-kappaB and STAT-1alpha and thereby activation of the iNOS promoter seems to be an essential step for the iNOS induction in most human cells. However, at least in the human system, also post-transcriptional mechanisms involving a complex network of RNA-binding proteins build up by AUF1, HuR, KSRP, PTB and TTP is critically involved in the regulation of iNOS expression. Recent data also implicate regulation of iNOS expression by non-coding RNAs (ncRNAs).
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In proteins, HOCl predominantly oxidizes cysteine and methionine residues to disulfide and methionine sulfoxide, respectively.
“Although HOCl reacts with a variety of biomolecule targets (755), in proteins, it predominantly oxidizes cysteine and methionine residues to disulfide and methionine sulfoxide, respectively (726).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [726]
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Kong, D., Ryu, J., Shin, N., Lee, S., Kim, N. G., Kim, H., Kim, M., Choi, J., Kim, D., & Kang, K. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202400919 · Cited by 36 · see citing works
Proteomic analysis of the NOS2 interactome in human airway epithelial cells
Foster, M. W., Thompson, J. W., Forrester, M. T., Sha, Y., McMahon, T. J., Bowles, D. E., Moseley, M. A., & Marshall, H. E. (2013) · Nitric Oxide · doi:10.1016/j.niox.2013.02.079 · Cited by 25 · see citing works
- Authors
- Pautz, A., Art, J., Hahn, S., Nowag, S., Voss, C., & Kleinert, H.
- Affiliations
- University Medical Center of the Johannes Gutenberg University Mainz (DE)
- Corresponding author
- Kleinert, Hartmut
- Venue
- Nitric Oxide
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 23
- Issue
- 2
- Pages
- 75-93
- ISSN
- 1089-8603 (ISSN-L)
- OpenAlex
- W2012154760
- Cited by
- 549
- References
- 221
- Citations by year
- 2026: 16 · 2025: 15 · 2024: 25 · 2023: 34 · 2022: 29 · 2021: 42 · 2020: 24 · 2019: 49 · 2018: 39 · 2017: 33 · 2016: 28 · 2015: 44 · 2014: 39 · 2013: 43 · 2012: 53
- FWCI
- 4.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nitric Oxide (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- inducible nitric oxide synthase, NF-κB, iNOS promoter, transcriptional regulation, post-transcriptional regulation, RNA-binding proteins, AUF1, HuR, KSRP, PTB, TTP, non-coding RNA, immunomodulation
- MeSH terms
- Humans, Promoter Regions, Genetic, RNA, Messenger, Transcription Factors, Gene Expression Regulation, Enzymologic, RNA-Binding Proteins, Nitric Oxide Synthase Type II
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