Facile Conjugation of Biomolecules onto Surfaces via Mussel Adhesive Protein Inspired Coatings
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A new surface bioconjugation strategy is presented. A polydopamine surface coating provides chemical activation on material surfaces, is resistant to hydrolysis, and offers selectivity in coupling of biomolecules via nucleophilic groups through simple pH control. Control of orientation of immobilized biomolecules may be possible using terminally modified DNA or His-containing proteins.
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Biomacromolecules such as proteins, peptides, and DNA can anchor on the surface of biosubstitutes via the polydopamine coating layer.
“In addition, biomacromolecules, such as proteins, peptides, and DNA, can anchor on the surface of biosubstitutes via the PDA coating layer [50, 57–60].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [58]
The main text (page not provided) reports immobilizing the protein trypsin and a model peptide on polydopamine-coated substrates. Its summary says the method “may prove useful” for immobilizing DNA; it does not report DNA attachment as an established result. (LLM, conf=0.86)
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Research works printed but not linked: 13
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- Authors
- Lee, H., Rho, J., & Messersmith, P. B.
- Affiliations
- Northwestern University (US)
- Corresponding author
- Messersmith, Phillip B.
- Venue
- Advanced Materials
- Publisher
- Wiley
- Published
- 2009-01-14
- Volume
- 21
- Issue
- 4
- Pages
- 431-434
- ISSN
- 0935-9648 (ISSN-L)
- 1521-4095
- PMC
- PMC2755254
- OpenAlex
- W2078797075
- Cited by
- 1,495
- References
- 0
- Citations by year
- 2026: 28 · 2025: 52 · 2024: 79 · 2023: 65 · 2022: 84 · 2021: 92 · 2020: 98 · 2019: 136 · 2018: 130 · 2017: 138 · 2016: 125 · 2015: 107 · 2014: 120 · 2013: 96 · 2012: 70
- FWCI
- 12.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Advanced Materials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Dental and Craniofacial Research (R37DE014193)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Physical Sciences › Materials Science › Biomaterials › Diatoms and Algae Research
- Keywords
- polydopamine coating, mussel adhesive proteins, biomolecule immobilization, nucleophilic coupling, hydrolytic stability
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