Non‐Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co‐Delivery of Cas9 mRNA and sgRNA

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

Genome engineering using the CRISPR-Cas9 system

Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013) · Nature Protocols · doi:10.1038/nprot.2013.143 · Cited by 11,133

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210

CRISPR-Cas systems for editing, regulating and targeting genomes

Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2842 · Cited by 3,153

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

Platt, R. J., Chen, S., Zhou, Y., Yim, M. J., Swiech, L., Kempton, H. R., Dahlman, J. E., Parnas, O., Eisenhaure, T. M., Jovanovic, M., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.014 · Cited by 2,001

Rational design of cationic lipids for siRNA delivery

Semple, S. C., Akinc, A., Chen, J., Sandhu, A. P., Mui, B. L., Cho, C. K., Sah, D. W. Y., Stebbing, D., Crosley, E. J., Yaworski, E., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt.1602 · Cited by 1,822

Delivery materials for siRNA therapeutics

Kanasty, R., Dorkin, J. R., Vegas, A., & Anderson, D. (2013) · Nature Materials · doi:10.1038/nmat3765 · Cited by 1,800

Image-based analysis of lipid nanoparticle–mediated siRNA delivery, intracellular trafficking and endosomal escape

Gilleron, J., Querbes, W., Zeigerer, A., Borodovsky, A., Marsico, G., Schubert, U., Manygoats, K., Seifert, S., Andree, C., Stöter, M., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2612 · Cited by 1,681

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, J. K., Chen, Z.-Y., & Liu, D. R. (2015) · Nature Biotechnology · doi:10.1038/nbt.3081 · Cited by 1,460

Nonviral Nanoparticles for CRISPR-Based Genome Editing: Is It Just a Simple Adaption of What Have Been Developed for Nucleic Acid Delivery?

Qiu, M., Glass, Z., & Xu, Q. (2019) · Biomacromolecules · doi:10.1021/acs.biomac.9b00783 · Cited by 30

Biomedically Relevant Applications of Bolaamphiphiles and Bolaamphiphile-Containing Materials

Hughes, J. R., Miller, A. S., Wallace, C. E., Vemuri, G. N., & Iovine, P. M. (2021) · Frontiers in Chemistry · doi:10.3389/fchem.2020.604151 · Cited by 30

Enhanced CRISPR-Cas9 RNA system delivery using cell penetrating peptides-based nanoparticles for efficient in vitro and in vivo applications

Gonzalez, V. G., Grunenberger, A., Nicoud, O., Czuba, E., Vollaire, J., Josserand, V., Guével, X. L., Desai, N., Coll, J., Divita, G., et al. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.11.008 · Cited by 29

CRISPR/Cas9-Based therapeutics as a promising strategy for management of Alzheimer’s disease: progress and prospects

Khan, M. S., Qureshi, N., Khan, R., Son, Y., & Maqbool, T. (2025) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2025.1578138 · Cited by 37

Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5′ cap-modified mRNAs

Ohno, H., Akamine, S., Mochizuki, M., Hayashi, K., Akichika, S., Suzuki, T., & Saito, H. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad019 · Cited by 31

Zinc Coordination Substitute Amine: A Noncationic Platform for Efficient and Safe Gene Delivery

Liu, S., Jia, H., Yang, J., Pan, J., Liang, H., Zeng, L., Zhou, H., Chen, J., & Guo, T. (2018) · ACS Macro Letters · doi:10.1021/acsmacrolett.8b00374 · Cited by 29

Nanomaterials-assisted gene editing and synthetic biology for optimizing the treatment of pulmonary diseases

Lei, L., Pan, W., Shou, X., Shao, Y., Ye, S., Zhang, J., Kolliputi, N., & Shi, L. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02627-w · Cited by 32

Enhanced delivery of CRISPR/Cas9 system based on biomimetic nanoparticles for hepatitis B virus therapy

Wu, K., He, M., Mao, B., Xing, Y., Wei, S., Jiang, D., Wang, S., Alkuhali, A. A., Guo, J., Gan, Z., et al. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.08.019 · Cited by 28

Dual SORT LNPs for multi-organ base editing

Kim, M., Song, E. S., Chen, J. C., Chatterjee, S., Sun, Y., Lee, S. M., Wu, S., Patel, P., Tian, Z., Kantor, A., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02675-z · Cited by 39

A CRISPR/Cas9 based polymeric nanoparticles to treat/inhibit microbial infections

Verma, R., Sahu, R., Singh, D. D., & Egbo, T. E. (2019) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2019.04.007 · Cited by 29

Recent advancements in exon-skipping therapies using antisense oligonucleotides and genome editing for the treatment of various muscular dystrophies

Hwang, J., & Yokota, T. (2019) · Expert Reviews in Molecular Medicine · doi:10.1017/erm.2019.5 · Cited by 29

A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types

Tarakanchikova, Y. V., Muslimov, A. R., Sergeev, I. S., Lepik, K. V., Yolshin, N. D., Goncharenko, A. A., Vasilyev, K., Eliseev, I. E., Bukatin, A., Sergeev, V. S., et al. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb01359e · Cited by 28

The evolution and history of gene editing technologies

Randhawa, S., & Sengar, S. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.002 · Cited by 31

mRNA therapeutics: Transforming medicine through innovation in design, delivery, and disease treatment

Ige, M. A., Ren, X., Yang, Y., Zhang, H., Shen, C., Jiang, Y., Li, J., & Wan, X. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102721 · Cited by 30

Design principles of lipid nanoparticles for RNA delivery

Arral, M. L., & Whitehead, K. A. (2026) · Nature Reviews Bioengineering · doi:10.1038/s44222-026-00401-1 · Cited by 41

Role of Vitronectin-Rich Protein Corona on Tumor-Specific siRNA Delivery and Transfection with Lipid Nanoparticles

Chen, D., Ganesh, S., Wang, W., Lupieri, A., & Amiji, M. M. (2021) · Nanomedicine · doi:10.2217/nnm-2020-0428 · Cited by 27

Development of Lipid Nanoparticles for the Delivery of Macromolecules Based on the Molecular Design of pH-Sensitive Cationic Lipids

Sato, Y. (2021) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.c21-00705 · Cited by 29

Ionizable drug delivery systems for efficient and selective gene therapy

Zhang, Y., Guo, R., Chen, Y., Li, T., Du, W.-Z., Xiang, R., Guan, J., Li, Y., Huang, Y., Yu, Z., et al. (2023) · Military Medical Research · doi:10.1186/s40779-023-00445-z · Cited by 27

Prime editing: current advances and therapeutic opportunities in human diseases

Fu, Y., He, X., Gao, X. D., Li, F., Ge, S., Yang, Z. H., & Fan, X. (2023) · 中国科学通报：英文版 · doi:10.1016/j.scib.2023.11.015 · Cited by 28

Thermo‐triggered Release of CRISPR‐Cas9 System by Lipid‐Encapsulated Gold Nanoparticles for Tumor Therapy

Wang, P., Zhang, L., Zheng, W., Cong, L., Guo, Z., Xie, Y., Wang, L., Tang, R., Feng, Q., Hamada, Y., et al. (2017) · Angewandte Chemie · doi:10.1002/ange.201708689 · Cited by 27

A combination of LightOn gene expression system and tumor microenvironment-responsive nanoparticle delivery system for targeted breast cancer therapy

Hou, X., Shou, C., He, M., Xu, J., Cheng, Y., Yuan, Z., Lan, M., Zhao, Y., Yang, Y. A., Chen, X., et al. (2020) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2020.04.010 · Cited by 26

Insights into the formulation of lipid nanoparticles for the optimization of mRNA therapeutics

Jiao, X., He, X., Qin, S., Yin, X., Song, T., Duan, X., Shi, H., Jiang, S., Zhang, Y., & Song, X. (2024) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.1992 · Cited by 28

Intelligent Design of Lipid Nanoparticles for Enhanced Gene Therapeutics

Yuan, Y., Li, Y., Li, G., Lei, L., Huang, X., Li, M., & Yao, Y. (2025) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.4c00925 · Cited by 28

Recent trends in CRISPR-Cas system: genome, epigenome, and transcriptome editing and CRISPR delivery systems

Bae, T., Hur, J. W., Kim, D., & Hur, J. K. (2019) · Genes & Genomics · doi:10.1007/s13258-019-00830-w · Cited by 26

Exploring Advanced CRISPR Delivery Technologies for Therapeutic Genome Editing

Rostami, N., Gomari, M. M., Choupani, E., Abkhiz, S., Fadaie, M., Eslami, S. S., Mahmoudi, Z., Zhang, Y., Puri, M., Monfared, F. N., et al. (2024) · Small Science · doi:10.1002/smsc.202400192 · Cited by 27

Green Transfection: Cationic Lipid Nanocarrier System Derivatized from Vegetable Fat, Palmstearin Enhances Nucleic Acid Transfections

Dharmalingam, P., Rachamalla, H. K., Lohchania, B., Bandlamudi, B. P., Thangavel, S., Mohankumar, K. M., Banerjee‬‬‬‬‬‬‬‬‬, R., Chaudhuri, A., Voshavar, C., & Marepally, S. K. (2017) · ACS Omega · doi:10.1021/acsomega.7b00935 · Cited by 25

Lipid and polymer mediated CRISPR/Cas9 gene editing

Gong, Y., Tian, S., Yang, X., & Zhang, S. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb00207k · Cited by 25

Context-Responsive Nanoparticle Derived from Synthetic Zwitterionic Ionizable Phospholipids in Targeted CRISPR/Cas9 Therapy for Basal-like Breast Cancer

Moitra, P., Skrodzki, D., Molinaro, M., Gunaseelan, N., Sar, D., Aditya, T., Dahal, D., Ray, P., & Pan, D. (2024) · ACS Nano · doi:10.1021/acsnano.4c01400 · Cited by 24

Biomaterials for Drug Delivery: Sources, Classification, Synthesis, Processing, and Applications

Adeosun, S. O., Ilomuanya, M. O., Gbenebor, O. P., Dada, M. O., & Odili, C. C. (2020) · IntechOpen eBooks · doi:10.5772/intechopen.93368 · Cited by 26

Nonviral Delivery of CRISPR/Cas Systems in mRNA Format

Liu, Z., Li, Z., & Li, B. (2022) · Advanced NanoBiomed Research · doi:10.1002/anbr.202200082 · Cited by 25

Strategies for organic nanoparticles delivering CRISPR/Cas9 for cancer therapy: Challenges and breakthroughs

Zhang, T., Ding, J., Lv, Q., Zhao, M., Liu, Y., Wang, Q., Chen, Y., Zhao, H., Ren, H., Jiang, W., et al. (2024) · Materials & Design · doi:10.1016/j.matdes.2024.113097 · Cited by 24

Therapeutic application of the CRISPR system: current issues and new prospects

Lee, M., & Kim, S. (2019) · Human Genetics · doi:10.1007/s00439-019-02028-2 · Cited by 23

Strategies for simultaneous and successive delivery of RNA

Moradian, H., Lendlein, A., & Gossen, M. (2020) · Journal of Molecular Medicine · doi:10.1007/s00109-020-01956-1 · Cited by 21

Efficient mRNA delivery using lipid nanoparticles modified with fusogenic coiled-coil peptides

Zeng, Y., Shen, M., Pattipeiluhu, R., Zhou, X., Zhang, Y., Bakkum, T., Sharp, T. H., Boyle, A. L., & Kros, A. (2023) · Nanoscale · doi:10.1039/d3nr02175k · Cited by 24

Hydrophobic Domain Structure of Linear-Dendritic Poly(ethylene glycol) Lipids Affects RNA Delivery of Lipid Nanoparticles

Zhou, K., Johnson, L. T., Xiong, H., Barrios, S., Minnig, J. T., Yan, Y., Abram, B., Yu, X., & Siegwart, D. J. (2020) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.9b01288 · Cited by 23

Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On‐ and Off‐Target Damage

Newman, A., Starrs, L. M., & Burgio, G. (2020) · BioEssays · doi:10.1002/bies.202000047 · Cited by 26

Understanding In Vivo Fate of Nucleic Acid and Gene Medicines for the Rational Design of Drugs

Fumoto, S., Yamamoto, T., Okami, K., Maemura, Y., Terada, C., Yamayoshi, A., & Nishida, K. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13020159 · Cited by 23

Spatiotemporal Delivery of CRISPR/Cas9 Genome Editing Machinery Using Stimuli‐Responsive Vehicles

Cai, W., Luo, T., Mao, L., & Wang, M. (2020) · Angewandte Chemie · doi:10.1002/ange.202005644 · Cited by 25

Translational research to enable personalized treatment of cystic fibrosis

Hagemeijer, M. C., Siegwart, D. J., Strug, L. J., Cebotaru, L., Torres, M. J., Sofoluwe, A., & Beekman, J. M. (2018) · Journal of Cystic Fibrosis · doi:10.1016/j.jcf.2017.10.017 · Cited by 22

CRISPR/dCas9—surface-enhanced Raman scattering for the detection of drug resistance gene macB

Du, Y., Han, D., An, Z., Wang, J., & Gao, Z. (2022) · Microchimica Acta · doi:10.1007/s00604-022-05460-w · Cited by 22

CES1‐Triggered Liver‐Specific Cargo Release of CRISPR/Cas9 Elements by Cationic Triadic Copolymeric Nanoparticles Targeting Gene Editing of PCSK9 for Hyperlipidemia Amelioration

Zhao, Y., Li, Y., Wang, F., Gan, X., Zheng, T., Chen, M., Li, W., Chen, J., & Yu, C. (2023) · Advanced Science · doi:10.1002/advs.202300502 · Cited by 22

Transformative therapies for rare CFTR missense alleles

Oliver, K. E., Han, S. T., Sorscher, E. J., & Cutting, G. R. (2017) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2017.09.018 · Cited by 23

Reversal of tumor malignization and modulation of cell behaviors through genome editing mediated by a multi-functional nanovector

Liu, B., He, X., Zhuo, R., & Cheng, S. (2018) · Nanoscale · doi:10.1039/c8nr07321j · Cited by 22

Polyrotaxane Nanocarriers Can Deliver CRISPR/Cas9 Plasmid to Dystrophic Muscle Cells to Successfully Edit the DMD Gene

Emami, M. R., Young, C. S., Ji, Y. C., Liu, X., Mokhonova, E. I., Pyle, A. D., Meng, H., & Spencer, M. J. (2019) · Advanced Therapeutics · doi:10.1002/adtp.201900061 · Cited by 22

Multivalent Peptide-Functionalized Bioreducible Polymers for Cellular Delivery of Various RNAs

Yang, D., Eldredge, A. C., Hickey, J. C., Muradyan, H., & Guan, Z. (2020) · Biomacromolecules · doi:10.1021/acs.biomac.0c00211 · Cited by 22

CRISPR-Associated (CAS) Effectors Delivery via Microfluidic Cell-Deformation Chip

Peyravian, N., Kebria, M. M., Kiani, J., Milan, P. B., & Mozafari, M. (2021) · Materials · doi:10.3390/ma14123164 · Cited by 22

Systems of Delivery of CRISPR/Cas9 Ribonucleoprotein Complexes for Genome Editing

Амирханов, Р. Н., & Stepanov, G. A. (2019) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s1068162019060025 · Cited by 21

Unlocking the Potential of Chemically Modified Nucleic Acid Therapeutics

Gao, J., Nutan, B., Gargouri, D., Pisal, N. D., Do, V., Zubair, M., Alanzi, H., Wang, H., Lee, D., Joshi, N., et al. (2024) · Advanced Therapeutics · doi:10.1002/adtp.202400231 · Cited by 20

Efficiency of Chitosan-Coated PLGA Nanocarriers for Cellular Delivery of siRNA and CRISPR/Cas9 Complex

Srivastav, A., Gupta, K., Chakraborty, D., Dandekar, P. P., & Jain, R. D. (2020) · Journal of Pharmaceutical Innovation · doi:10.1007/s12247-020-09496-4 · Cited by 21

In vivo genome editing at the albumin locus to treat methylmalonic acidemia

Schneller, J. L., Lee, C. M., Venturoni, L. E., Chandler, R. J., Li, A., Myung, S., Cradick, T. J., Hurley, A. E., Lagor, W. R., Bao, G., et al. (2021) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2021.11.004 · Cited by 20

The Potential of Nanomedicine to Unlock the Limitless Applications of mRNA

Taina-González, L., & de la Fuente, M. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14020460 · Cited by 23

Microfluidic one-step synthesis of a metal−organic framework for osteoarthritis therapeutic microRNAs delivery

Yang, K., Ni, M., Xu, C., Wang, L., Han, L., Lv, S., Wu, W., & Zheng, D. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1239364 · Cited by 20

Hydrophobic Optimization of Functional Poly(TPAE-co-suberoyl chloride) for Extrahepatic mRNA Delivery following Intravenous Administration

Yu, X., Liu, S., Cheng, Q., Lee, S. M., Wei, T., Zhang, D., Farbiak, L., Johnson, L. T., Wang, X., & Siegwart, D. J. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13111914 · Cited by 20

Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems

Lokras, A. G., Chakravarty, A., Rades, T., Christensen, D., Franzyk, H., Thakur, A., & Foged, C. (2022) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2022.122171 · Cited by 23

Current knowledge on the tissue distribution of mRNA nanocarriers for therapeutic protein expression

Zadory, M., Lopez, E., Babity, S., Gravel, S., & Brambilla, D. (2022) · Biomaterials Science · doi:10.1039/d2bm00859a · Cited by 18

Debugging the genetic code: Non-viral in vivo delivery of therapeutic genome editing technologies

Piotrowski-Daspit, A. S., Glazer, P. M., & Saltzman, W. M. (2018) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2018.08.002 · Cited by 19

In vitro transcribed mRNA for expression of designer nucleases: Advantages as a novel therapeutic for the management of chronic HBV infection

Ely, A., Singh, P., Smith, T. S., & Arbuthnot, P. B. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.05.010 · Cited by 19

Designing biomaterials for the delivery of RNA therapeutics to stimulate bone healing

Andrée, L., Yang, F., Brock, R. E., & Leeuwenburgh, S. C. G. (2021) · Materials Today Bio · doi:10.1016/j.mtbio.2021.100105 · Cited by 21

Protocol for Delivery of CRISPR/dCas9 Systems for Epigenetic Editing into Solid Tumors Using Lipid Nanoparticles Encapsulating RNA

Woodward, E., Wang, E., Wallis, C. J. D., Sharma, R. K., Tie, A. W. J., Murthy, N., & Blancafort, P. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-4051-7_14 · Cited by 18

Dual-use nano-neurotechnology

Nixdorff, K., Borisova, Т., Комісаренко, С. В., & Dando, M. (2018) · Politics and the Life Sciences · doi:10.1017/pls.2018.15 · Cited by 20

CRISPR to the Rescue: Advances in Gene Editing for the FMR1 Gene

Yrigollen, C. M., & Davidson, B. L. (2019) · Brain Sciences · doi:10.3390/brainsci9010017 · Cited by 19

Mannose-Conjugated Cholesterol Containing Lipid Nanoparticles for Active Targeted mRNA Delivery to Liver Sinusoidal Endothelial and Kupffer Cells

Kim, Y., Chatterjee, S., Robertson, A. L., Guerrero, E. D., Vaidya, A., Wang, X., Lee, S. M., Wei, J., Miller, W. E., Farbiak, L., et al. (2025) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.5c00282 · Cited by 18

Hypoxia‐Responsive Gene Editing to Reduce Tumor Thermal Tolerance for Mild‐Photothermal Therapy

Li, X., Pan, Y., Chen, C., Gao, Y., Liu, X., Yang, K., Luan, X., Zhou, D., Zeng, F., Han, X., et al. (2021) · Angewandte Chemie · doi:10.1002/ange.202107036 · Cited by 17

Customizable Lipid Nanoparticle Materials for the Delivery of siRNAs and mRNAs

Fenton, O. S., Kauffman, K. J., McClellan, R. L., Kaczmarek, J. C., Zeng, M. D., Andresen, J. L., Rhym, L. H., Heartlein, M. W., DeRosa, F., & Anderson, D. G. (2018) · Angewandte Chemie · doi:10.1002/ange.201809056 · Cited by 18

Engineered PsCas9 enables therapeutic genome editing in mouse liver with lipid nanoparticles

Degtev, D., Bravo, J. P. K., Emmanouilidi, A., Zdravković, A., Choong, O. K., Touza, J. L., Selfjord, N., Weisheit, I., Francescatto, M., Akçakaya, P., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-53418-8 · Cited by 19

Nanotechnological approaches for the targeted delivery of CRISPR-Cas systems for genomic modifications, biomolecular sensing, and precision medicine

Baig, M. M. F. A., Chien, W. T., & Chair, S. Y. (2025) · Biomaterials Science · doi:10.1039/d5bm00711a · Cited by 19

Lipid Nanoparticle Database towards structure-function modeling and data-driven design for nucleic acid delivery

Collins, E., Ji, J., Kim, S.-G., Witten, J. J., Kim, S., Zhu, R., Park, P., Jung, M., Park, A., Manan, R. S., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-68818-1 · Cited by 26

In situ production and secretion of proteins endow therapeutic benefit against psoriasiform dermatitis and melanoma

Cheng, Q., Farbiak, L., Vaidya, A., Guerrero, E. D., Lee, E. E., Rose, E. K., Wang, X., Robinson, J. J., Lee, S. M., Wei, T., et al. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2313009120 · Cited by 17

Triple‐Targeting Delivery of CRISPR/Cas9 To Reduce the Risk of Cardiovascular Diseases

Zhang, L., Wang, L., Xie, Y., Wang, P., Deng, S., Qin, A., Zhang, J., Yu, X., Zheng, W., & Jiang, X. (2019) · Angewandte Chemie · doi:10.1002/ange.201903618 · Cited by 15

Fabrication of Chlorophyll-Incorporated Nanogels for Potential Applications in Photothermal Cancer Therapy

Chang, R., Hsu, C.-F., & Tsai, W. (2018) · ACS Omega · doi:10.1021/acsomega.8b01689 · Cited by 16

Engineering CRISPR-Cas9 RNA–Protein Complexes for Improved Function and Delivery

Rouet, R., de Oñate, L., Li, J., Murthy, N., & Wilson, R. C. (2018) · The CRISPR Journal · doi:10.1089/crispr.2018.0037 · Cited by 16

mRNA delivery systems 2.0: Engineering extrahepatic delivery for non-vaccine therapeutics

Dalabehera, M., Ghosh, A., Mohanty, S., Chellappan, D. K., Chaudhari, S., Ale, Y., Poonia, N., Subudhi, R. N., Khanna, M., & Lim, H. G. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.102584 · Cited by 22

Nanoparticle-based non-viral CRISPR delivery for enhanced immunotherapy

Shin, H., & Kim, J. (2022) · Chemical Communications · doi:10.1039/d1cc05999h · Cited by 14

An Ultrahydrating Polymer that Protects Protein Therapeutics and RNA‐Lipid Nanoparticles Against Freezing, Heat and Lyophilization Stress

Herrmann, A., Abbina, S., Bathula, N. V., Nouri, P. M. M., Chafeeva, I., Constantinescu, I., Abbasi, E., Abbasi, U., Drayton, M., Luo, H. D., et al. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202406878 · Cited by 15

Cisplatin and Ce6 loaded polyaniline nanoparticles: An efficient near-infrared light mediated synergistic therapeutic agent

You, C., Gao, Z., Wang, M., Wu, H., An, P., Wang, S., Sun, Y., Sun, B., & Zhang, X. (2018) · Materials Science and Engineering C · doi:10.1016/j.msec.2018.03.031 · Cited by 15

Supramolecular Nanosubstrate‐Mediated Delivery for CRISPR/Cas9 Gene Disruption and Deletion

Ban, Q., Yang, P., Chou, S., Qiao, L., Xia, H., Xue, J., Wang, F., Xu, X., Sun, N., Zhang, R. Y., et al. (2021) · Small · doi:10.1002/smll.202100546 · Cited by 16

Latest progress in the study of nanoparticle-based delivery of the CRISPR/Cas9 system

Yu, M., Liu, X., Cheng, H., Kuang, L., Zhang, S., & Yan, X. (2021) · Methods · doi:10.1016/j.ymeth.2021.06.004 · Cited by 15

Co-delivery of mRNA and SPIONs through amino-ester nanomaterials

Luo, X. L., Zhao, W., Li, B., Zhang, X., Zhang, C., Bratasz, A., Deng, B., McComb, D. W., & Dong, Y. (2018) · Nano Research · doi:10.1007/s12274-018-2082-0 · Cited by 15

Viral and nonviral nanocarriers for in vivo CRISPR-based gene editing

Guo, Z., Zhu, A. T., Fang, R. H., & Zhang, L. (2024) · Nano Research · doi:10.1007/s12274-024-6748-5 · Cited by 18

Engineering stimuli-responsive nanocarriers for CRISPR/Cas9 genome editing: next-generation cancer therapeutics

Mehnath, S. (2025) · Journal of Pharmacy and Pharmacology · doi:10.1093/jpp/rgaf127 · Cited by 14

Autonomous Feedback-Driven Engineered DNAzyme-Coated Trojan Horse-like Nanocapsules for On-Demand CRISPR/Cas9 Delivery

Tang, X., Chen, Y., Wang, B., Luo, D., Wang, J., He, Y., Feng, L., Xu, Y. X., Xie, S., Chen, M., et al. (2024) · ACS Nano · doi:10.1021/acsnano.4c04147 · Cited by 15

Advances in Development of mRNA-Based Therapeutics

Huang, L., Zhang, L., Li, W., Li, S., Wen, J., Li, H., & Liu, Z. (2020) · Current topics in microbiology and immunology · doi:10.1007/82_2020_222 · Cited by 16

Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics

Li, X., Feng, Q., Han, Z., & Jiang, X. (2021) · Chinese Journal of Chemical Engineering · doi:10.1016/j.cjche.2021.02.009 · Cited by 14

Recent advances in research on biointerfaces: From cell surfaces to artificial interfaces

Hori, K., Yoshimoto, S., Yoshino, T., Zako, T., Hirao, G., Fujita, S., Nakamura, C., Yamagishi, A., & Kamiya, N. (2022) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2021.12.004 · Cited by 14

Current Advances Toward the Encapsulation of Cas9

Kanduri, V., LaVigne, D., & Larsen, J. (2021) · ACS Macro Letters · doi:10.1021/acsmacrolett.1c00538 · Cited by 14

Preclinical Advances in LNP-CRISPR Therapeutics for Solid Tumor Treatment

Wang, S., Zhu, Y., Du, S., & Zheng, Y. (2024) · Cells · doi:10.3390/cells13070568 · Cited by 16

Cell and Gene Therapies for Mucopolysaccharidoses: Base Editing and Therapeutic Delivery to the CNS

Christensen, C. L., Ashmead, R. E., & Choy, F. Y. M. (2019) · Diseases · doi:10.3390/diseases7030047 · Cited by 13

Constructing a Ready-to-Use mRNA Vaccine Delivery System for the Prevention of Influenza A virus, Utilizing FDA-Approved Raw Materials

Zhang, Y., Zhuang, X., Hu, Y., Chen, J., Hao, K., Tang, Z., Tian, M., Tian, H., Jin, N., & Chen, X. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.4c00365 · Cited by 12

Essential cues of engineered polymeric materials regulating gene transfer pathways

Islam, M. A., Park, T., Firdous, J., Li, H.-S., Jiménez-Pérez, Z. E., Lim, M., Choi, J.-W., Yun, C., & Cho, C. (2022) · Progress in Materials Science · doi:10.1016/j.pmatsci.2022.100961 · Cited by 12

Investigating the functional contributions of phospholipids in selective organ targeting lipid nanoparticles

Guerrero, E. D., Vaidya, A., Santelli, J., Tian, Z., Pazzi, G. A., & Siegwart, D. J. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123671 · Cited by 11

Autonomous Nucleic Acid and Protein Nanocomputing Agents Engineered to Operate in Living Cells

Panigaj, M., Roy, T. B., Skelly, E., Chandler, M. R., Wang, J., Ekambaram, S., Bircsak, K. M., Dokholyan, N. V., & Afonin, K. A. (2025) · ACS Nano · doi:10.1021/acsnano.4c13663 · Cited by 13

Lipid Nanoparticles for mRNA Delivery in Cancer Immunotherapy

Alshehry, Y., Liu, X., Li, W., Wang, Q., Cole, J., & Zhu, G. (2025) · The AAPS Journal · doi:10.1208/s12248-025-01051-8 · Cited by 13

Dramatic influence of the hydroxy functionality of azasugar moiety in the head group region of tocopherol-based cationic lipids on in vitro gene transfection efficacies

Muripiti, V., Lohchania, B., Ravula, V., Manturthi, S., Marepally, S. K., Velidandi, A., & Patri, S. V. (2020) · New Journal of Chemistry · doi:10.1039/d0nj03717f · Cited by 11

Saponin and fluorine-modified polycation as a versatile gene delivery system

Hasanzadeh, A., Vahabi, A. H., Hooshmand, S. E., Hosseini, E. S., Azar, B. K. Y., Kiani, J., Saeedi, S., Shahbazi, A., Rudra, A., Hamblin, M. R., et al. (2022) · Nanotechnology · doi:10.1088/1361-6528/ac842d · Cited by 10

Development and Application of Automated Sandwich ELISA for Quantitating Residual dsRNA in mRNA Vaccines

Holland, D. A., Acevedo‐Skrip, J., Barton, J. R., Thompson, R., Bowman, A. M., Dewar, E. A., Miller, D. V., Zhao, K., Swartz, A. R., & Loughney, J. W. (2024) · Vaccines · doi:10.3390/vaccines12080899 · Cited by 10

Reversible switching of primary cells between normal and malignant state by oncogenic virus KSHV and CRISPR/Cas9‐mediated targeting of a major viral latent protein

Ju, E., Li, T., da Silva, S. R., Markazi, A. D., & Gao, S. (2021) · Journal of Medical Virology · doi:10.1002/jmv.27046 · Cited by 10

Sensors for agricultural pest management using nanotechnology/nanomaterials

Srujana, S., & Bhagat, D. (2022) · Nanotechnology for Environmental Engineering · doi:10.1007/s41204-022-00241-5 · Cited by 10

Selecting a Cell Engineering Methodology During Cell Therapy Product Development

Timmins, L. M., Burr, A., Carroll, K., Keefe, R., Teryek, M., Cantolupo, L. J., van der Loo, J. C. M., Heathman, T. R. J., Gormley, A. J., Smith, D. W., et al. (2021) · Cell Transplantation · doi:10.1177/09636897211003022 · Cited by 10

Genome editing: An essential technology for cancer treatment

Tufail, M. (2022) · Medicine in Omics · doi:10.1016/j.meomic.2022.100015 · Cited by 10

Green Synthesis of Zinc Oxide Nanoparticles Mediated from Cassia renigera Bark and Detect Its Effects on Four Varieties of Rice

Parveen, K., Kumar, N., & Ledwani, L. (2022) · ChemistrySelect · doi:10.1002/slct.202200415 · Cited by 10

Engineering of Ionizable Lipid Nanoparticle for Efficient Crispr-Cas9 Delivery and Genome EditingPreprint

Sun, Q., Zhang, H., Ding, F., Zhu, Z., & Yang, C. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4476006

Comparative analysis of lipid Nanoparticle-Mediated delivery of CRISPR-Cas9 RNP versus mRNA/sgRNA for gene editing in vitro and in vivo

Walther, J., Porenta, D., Wilbie, D., Seinen, C. W., Benne, N., Yang, Q., de Jong, O. G., Lei, Z., & Mastrobattista, E. (2024) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2024.114207 · Cited by 80

Development of ionizable lipid nanoparticles and a lyophilized formulation for potent CRISPR-Cas9 delivery and genome editing

Sun, Q., Zhang, H., Ding, F., Gao, X., Zhu, Z., & Yang, C. (2024) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2024.123845 · Cited by 18

Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles

Liu, J., Chang, J., Jiang, Y., Meng, X., Sun, T., Mao, L., Xu, Q., & Wang, M. (2019) · Advanced Materials · doi:10.1002/adma.201902575 · Cited by 373

Finely tuned ionizable lipid nanoparticles for CRISPR/Cas9 ribonucleoprotein delivery and gene editing

Im, S. H., Jang, M., Park, J., & Chung, H. J. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02427-2 · Cited by 43