Separation of the polysaccharides in Caulerpa racemosa and their chemical composition and antitumor activity
Abstract
Abstract Caulerpa racemosa was extracted with the combined procedure of neutral protease and boiling water to yield a water‐soluble polysaccharide coded as CRP. The obtained C. racemosa polysaccharide (CRP) was fractionated with DEAE‐52 cellulose, which led to two soluble polysaccharide fractions designated as CRPF1 and CRPF2. CRP, CRPF1, and CRPF2 were chemically determined and their antitumor activity was assayed. Results showed that they were all mixtures composed of sulfated polysaccharide with 3.9–7.9% uronic acid and protein, and had similar amino acid composition, but CRP was more than CRPF1 and CRPF2 in total sugar and uronic acid content, and less in sulfate group content. Two main sugars, glucose (Glu, 56.8%) and galactose (Gal, 31.8%), of CRP were enriched to CRPF2 (Glu, 89.1%) and CRPF1 (Gal, 99.2%) respectively; Bioassay indicated that all CRP had strong antitumor activity in both in vitro and in vivo, and its inhibition rate of K562 cells in vitro at the concentration of 6.0–10.0 mg mL−1 and of H22 tumor transplanted in mice at a dose of 100 mg kg−1 day were 59.5–83.8% (48 h) and 53.9% (14 days), respectively. Moreover, at a lower dose (0.05–0.2 mg mL−1) and longer time (72 h), CRP exhibited stronger inhibition effect on K562 cells than CRPF1 and CRPF2. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
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Promising antioxidant and antiproliferative activities were found in sulfated polysaccharides isolated from several tropical species of green algae.
“Promising antioxidant and antiproliferative activities were recently found in the sulfated polysaccharides isolated from several tropical species of green algae.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [108]
Two polysaccharide fractions from Caulerpa racemosa showed antitumor activities with inhibition rates of H22 tumor transplanted in mice of 59.5%-83.8% at 48 h and 53.9% at 14 days at a dose of 100 mg/kg/day.
“Two polysaccharide fractions obtained from the green alga Caulerpa racemosa showed antitumor activities, and their inhibition rates of H22 tumor transplanted in mice were 59.5%–83.8% (48 h) and 53.9% (14 days) at a dose of 100 mg/kg/day, respectively [108].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [108]
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Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
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- Authors
- Ji, H., Shao, H., Zhang, C., Hong, P., & Xiong, H.
- Affiliations
- Guangdong Ocean University (CN)
- Corresponding author
- Ji, Hongwu
- ORCID
- Hong, Pengzhi
- Venue
- Journal of Applied Polymer Science
- Publisher
- Wiley
- Published
- 2008-07-17
- Volume
- 110
- Issue
- 3
- Pages
- 1435-1440
- ISSN
- 0021-8995 (ISSN-L)
- 1097-4628
- OpenAlex
- W1994049875
- Cited by
- 56
- References
- 13
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 5 · 2019: 5 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 3 · 2014: 7 · 2013: 6 · 2012: 4
- FWCI
- 2.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Polymer Science (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- Caulerpa racemosa, sulfated polysaccharides, polysaccharide fractionation, uronic acid, glucose, galactose, anticancer activity, K562 cells, H22 hepatocellular carcinoma, seaweed polysaccharides, polysaccharide composition
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