The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
A new, mild cross‐linking methodology to prepare cross‐linked enzyme aggregates
Abstract
Cross‐linked enzyme aggregates (CLEAs) were prepared from several enzymes (penicillin G acylase, hydroxynitrile lyase, alcohol dehydrogenase, and two different nitrilases) by precipitation and subsequent cross‐linking using dextran polyaldehyde. In most cases, higher immobilization yields were obtained using the latter cross‐linker as compared with the commonly used glutaraldehyde. Active site titration of penicillin acylase CLEAs showed that the higher activity originated from a significantly lower loss in active sites using dextran polyaldehyde as a cross‐linking agent. It is proposed that macromolecular cross‐linkers are too large to penetrate the protein active site and react with catalytically essential amino acid residues. © 2004 Wiley Periodicals, Inc.
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Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes
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Self-citationINTERMOLECULAR CROSS LINKING OF A PROTEIN IN THE CRYSTALLINE STATE: CARBOXYPEPTIDASE-A
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Cross-linked enzyme crystals as robust biocatalysts
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Cross-linked enzyme aggregates with enhanced activity: application to lipases
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Self-citationPreparation of enzymically active, water-insoluble derivatives of trypsin
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Enzyme immobilisation in biocatalysis: why, what and how
Sheldon, R. A., & van Pelt, S. (2013) · Chem. Soc. Rev. · doi:10.1039/c3cs60075k · Cited by 2,675
Self-citationEnzyme Immobilization: The Quest for Optimum Performance
Sheldon, R. A. (2007) · Advanced Synthesis & Catalysis · doi:10.1002/adsc.200700082 · Cited by 2,077
Self-citationImmobilization of enzymes on porous silicas – benefits and challenges
Hartmann, M., & Kostrov, X. (2013) · Chemical Society Reviews · doi:10.1039/c3cs60021a · Cited by 605
Cross-linked enzyme aggregates (CLEA®s): stable and recyclable biocatalysts
Sheldon, R. A. (2007) · Biochemical Society Transactions · doi:10.1042/bst0351583 · Cited by 422
Self-citationMethods for stabilizing and activating enzymes in ionic liquids—a review
Zhao, H. (2010) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2375 · Cited by 351
Metadata
- Authors
- Mateo, C., Palomo, J. M., van Langen, L. M., van Rantwijk, F., & Sheldon, R. A.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2004-03-11
- Volume
- 86
- Issue
- 3
- Pages
- 273-276
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2121758307
- Cited by
- 300
- References
- 14
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
