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Cited by 177

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Monitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy

Pistorius, Arthur M.A., DeGrip, Willem J., Egorova‐Zachernyuk, Tatjana A.·2009·Biotechnology and Bioengineering
DOI 10.1002/bit.22220
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Abstract

An FT‐IR spectroscopic method was developed for the simultaneous quantitative analysis of biomacromolecular components in biomass, originating from various microbiological sources. For the determination of protein, lipid and carbohydrate content, creatine phosphokinase, egg phosphatidyl choline and starch hydrolysate were chosen as external standards. This selection was based on spectral similarity and ease of availability. Protein content was based on the area under the amide II band profile around 1,545 cm−1. Because of the heterogeneous lipid composition in the different species, lipid content was determined using integration over the CH stretching vibrational population between 2,984 and 2,780 cm−1. Carbohydrate content was determined using integration over a CO and COC stretching band area between 1,180 and 1,133 cm−1. Linear regression analysis provided three calibration lines, according to which biomasses from ten species were analyzed. This approach showed good intra‐batch reproducibility. With this method we could demonstrate good reproducibility between batches of the same species with similar growth conditions while large differences in biomass composition were observed between the various species. Protein content as determined by FT‐IR spectroscopy compared well with the results obtained from elemental analysis. Biotechnol. Bioeng. 2009;103: 123–129. © 2008 Wiley Periodicals, Inc.

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Research works printed but not linked: 25

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Metadata

Authors
Pistorius, A. M. A., DeGrip, W. J., & Egorova‐Zachernyuk, T. A.
Venue
Biotechnology and Bioengineering
Publisher
Wiley
Published
2009-01-08
Volume
103
Issue
1
Pages
123-129
ISSN
  • 0006-3592 (ISSN-L)
  • 1097-0290
DOI
10.1002/bit.22220
OpenAlex
W2078752137
Cited by
177
References
1
FWCI
4.52
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)

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