The use of FTIR spectroscopy to assess quantitative changes in the biochemical composition of microalgae
Abstract
A mid-infrared spectroscopic method was developed for the simultaneous and quantitative determination of total protein, carbohydrate and lipid contents of microalgal cells. Based on a chemometric approach, measured FTIR (Fourier transform infrared) spectra from algal cells were reconstructed by a partial least square algorithm, using the spectra of the reference substances to determine their relative contribution to the overall cell spectrum. From this specific absorption, absolute macromolecular cell composition [pg cell(-1)] can be calculated using calibration curves, which have been validated by independent biochemical methods. The future potential of this method for photosynthesis research is shown by its application to follow time-resolved changes in the cellular composition of microalgae during an illumination period of several hours. We show how the macromolecular composition can be investigated by FTIR spectroscopy methods. This can substantially increase the efficiency of screening processes like bioreactor monitoring and may be beneficial in metabolic engineering of algal cells.
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- Authors
- Wagner, H., Liu, Z., Langner, U., Stehfest, K., & Wilhelm, C.
- Affiliations
- Leipzig University (DE)
- Martin Luther University Halle-Wittenberg (DE)
- Humboldt-Universität zu Berlin (DE)
- Corresponding author
- Wilhelm, Christian
- Venue
- Journal of Biophotonics
- Publisher
- Wiley
- Published
- 2010-07-19
- Volume
- 3
- Issue
- 8-9
- Pages
- 557-566
- ISSN
- 1864-063X (ISSN-L)
- 1864-0648
- OpenAlex
- W1998023097
- Cited by
- 134
- References
- 34
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 12 · 2023: 9 · 2022: 4 · 2021: 12 · 2020: 15 · 2019: 15 · 2018: 8 · 2017: 9 · 2016: 4 · 2015: 5 · 2014: 9 · 2013: 9 · 2012: 8
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biophotonics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Centre National de la Recherche Scientifique
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- microalgae, FTIR spectroscopy, mid-infrared spectroscopy, partial least squares, protein content, carbohydrate content, lipid content, macromolecular composition, chemometrics, photosynthesis, bioreactor monitoring
- MeSH terms
- Bacterial Proteins, Chlamydomonas reinhardtii, Spectroscopy, Fourier Transform Infrared, Microalgae
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