Rapid determination of bulk microalgal biochemical composition by Fourier-Transform Infrared spectroscopy
Abstract
Analysis of bulk biochemical composition is a key in fundamental and applied studies of microalgae and is essential to understanding responses to different cultivation scenarios. Traditional biochemical methods for the quantification of lipids, carbohydrates and proteins are often time-consuming, often involve hazardous reagents, require significant amounts of biomass and are highly dependent on practitioner proficiency. This study presents a rapid and non-destructive method, utilising Fourier-Transform Infrared (FTIR) spectroscopy for the simultaneous determination of lipid, protein and carbohydrate content in microalgal biomass. A simple univariate regression was applied to sets of reference microalgal spectra of known composition and recognised IR peak integrals. A robust single-species model was constructed, with coefficients of determination r(2)>0.95, high predictive accuracy and relative errors below 5%. The applicability of this methodology is demonstrated by monitoring the time-resolved changes in biochemical composition of the marine alga Nannochloropsis sp. grown to nitrogen starvation.
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FTIR spectroscopy has been used for rapid assessment of lipids without lipid extraction and to monitor changes within intracellular macromolecular pools of microalgae.
“Many of the studies have reported the use of FTIR spectroscopy in the rapid assessment of lipids, without the need for lipid extraction and to monitor the changes within the intracellular macromolecular pools of microalgae [15,20 -23].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [22]
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- Authors
- Mayers, J. J., Flynn, K. J., & Shields, R. J.
- Affiliations
- Swansea University (GB)
- Corresponding author
- Flynn, Kevin J.
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2013-11
- Volume
- 148
- Pages
- 215-220
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W1965058293
- Cited by
- 179
- References
- 37
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 14 · 2023: 17 · 2022: 10 · 2021: 27 · 2020: 18 · 2019: 23 · 2018: 8 · 2017: 13 · 2016: 15 · 2015: 6 · 2014: 7
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Chalmers Publication Library (Chalmers University of Technology) (submittedVersion)
- Chalmers Research (Chalmers University of Technology) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- microalgal biomass, biochemical composition, lipid content, protein content, carbohydrate content, FTIR spectroscopy, non-destructive analysis, univariate regression, Nannochloropsis, nitrogen starvation
- MeSH terms
- Absorption, Carbohydrates, Carbon, Lipids, Models, Theoretical, Nitrogen, Proteins, Reference Standards, Time Factors, Spectroscopy, Fourier Transform Infrared, Biomass, Microalgae, Batch Cell Culture Techniques
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