Effect of light intensity on algal biomass accumulation and biodiesel production for mixotrophic strains Chlorella kessleri and Chlorella protothecoide cultivated in highly concentrated municipal wastewater
Abstract
In this research, the effect of light intensity on biomass accumulation, wastewater nutrient removal through algae cultivation, and biodiesel productivity was investigated with algae species Chlorella kessleri and Chlorella protothecoide. The light intensities studied were 0, 15, 30, 60, 120, and 200 µmol m(-2) s(-1). The results showed that light intensity had profound impact on tested responses for both strains, and the dependence of these responses on light intensity varied with different algae strains. For C. kessleri, the optimum light intensity was 120 µmol m(-2) S(-1) for all responses except for COD removal. For C. protothecoide, the optimum light intensity was 30 µmol m(-2) S(-1). The major components of the biodiesel produced from algae biomass were 16-C and 18-C FAME, and the highest biodiesel contents were 24.19% and 19.48% of dried biomass for C. kessleri and C. protothecoide, respectively. Both species were capable of wastewater nutrients removal under all lighting conditions with high removal efficiencies.
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In outdoor microalgal culture systems where high light intensity of sunlight is used, only those species of Chlorella that are not sensitive to photo-inhibition of organic carbon uptake would be suitable for mixotrophic cultivation.
“Therefore, in outdoor microalgal culture systems where high light intensity of sunlight is used, only those species of Chlorella that are no sensitive to photo-inhibition of the organic carbon uptake would be suitable for mixotrophic cultivation [37].”
From Challenges and Opportunities to Increase Economic Feasibility and Sustainability of Mixotrophic Cultivation of Green Microalgae of the Genus Chlorella (2022) · cites this paper as [37]
High removal efficiencies of nutrients from centrate were found when C. kessleri and C. protothecoides were cultivated under mixotrophic conditions aiming simultaneous wastewater treatment and biodiesel production.
“High removal efficiencies of nutrients from centrate were found when C. kessleri and C. protothecoides were cultivated under mixotrophic conditions aiming simultaneous wastewater treatment and biodiesel production [37].”
From Challenges and Opportunities to Increase Economic Feasibility and Sustainability of Mixotrophic Cultivation of Green Microalgae of the Genus Chlorella (2022) · cites this paper as [37]
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- Authors
- Li, Y., Zhou, W., Hu, B., Min, M., Chen, P., & Ruan, R. R.
- Affiliations
- University of Minnesota (US)
- Corresponding author
- Ruan, Roger R.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2012-03-22
- Volume
- 109
- Issue
- 9
- Pages
- 2222-2229
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2150284398
- Cited by
- 144
- References
- 24
- Citations by year
- 2026: 9 · 2025: 4 · 2024: 8 · 2023: 5 · 2022: 12 · 2021: 9 · 2020: 14 · 2019: 19 · 2018: 12 · 2017: 9 · 2016: 5 · 2015: 12 · 2014: 14 · 2013: 11 · 2012: 1
- FWCI
- 4.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Environmental Science › Waste Management and Disposal › Wastewater Treatment and Reuse
- Keywords
- light intensity, Chlorella kessleri, mixotrophic cultivation, biodiesel production, municipal wastewater treatment, nutrient removal, fatty acid methyl esters, COD removal, microalgae
- MeSH terms
- Chlorella, Light, Sewage, Waste Disposal, Fluid, Biomass, Biofuels
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