The effects of garlic-derived sulfur compounds on cell proliferation, caspase 3 activity, thiol levels and anaerobic sulfur metabolism in human hepatoblastoma HepG2 cells
Open accessAbstract
The aim of the present studies was to determine whether the mechanism of biological action of garlic‐derived sulfur compounds in human hepatoma (HepG2) cells can be dependent on the presence of labile sulfane sulfur in their molecules. We investigated the effect of allyl sulfides from garlic: monosulfide, disulfide and trisulfide on cell proliferation and viability, caspase 3 activity and hydrogen peroxide (H 2 O 2) production in HepG2 cells. In parallel, we also examined the influence of the previously mentioned compounds on the levels of thiols, glutathione, cysteine and cysteinyl‐glycine, and on the level of sulfane sulfur and the activity of its metabolic enzymes: rhodanese, 3‐mercaptopyruvate sulfurtransferase and cystathionase. Among the compounds under study, diallyl trisulfide (DATS), a sulfane sulfur‐containing compound, showed the highest biological activity in HepG2 cells. This compound increased the H 2 O 2 formation, lowered the thiol level and produced the strongest inhibition of cell proliferation and the greatest induction of caspase 3 activity in HepG2 cells. DATS did not affect the activity of sulfurtransferases and lowered sulfane sulfur level in HepG2 cells. It appears that sulfane sulfur containing DATS can be bioreduced in cancer cells to hydroperthiol that leads to H 2 O 2 generation, thereby influencing transmission of signals regulating cell proliferation and apoptosis. Copyright © 2011 John Wiley & Sons, Ltd.
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Organo-sulfur compounds (OSC) including allicin, DADS, alliin, DAS, allyl mercaptan (AM), and S-allyl cysteine (SAC) isolated from garlic have anti-tumor properties.
“Iciek et al. [83] have reported the anti-tumor properties of organo-sulfur compounds (OSC) including allicin, DADS, alliin, DAS, allyl mercaptan (AM), and S-allyl cysteine (SAC), isolated from garlic.”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [83]
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Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review
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- Authors
- Iciek, M., Kwiecień, I., Chwatko, G., Sokołowska‐Jeżewicz, M., Kowalczyk‐Pachel, D., & Rokita, H.
- Affiliations
- Jagiellonian University (PL)
- University of Łódź (PL)
- Corresponding author
- Iciek, Małgorzata
- Venue
- Cell Biochemistry and Function
- Publisher
- Wiley
- Published
- 2011-11-18
- Volume
- 30
- Issue
- 3
- Pages
- 198-204
- ISSN
- 0263-6484 (ISSN-L)
- 1099-0844
- OpenAlex
- W1845640203
- Cited by
- 62
- References
- 33
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 8 · 2020: 6 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 6 · 2014: 4 · 2013: 2 · 2012: 5
- FWCI
- 4.24
- Type
- journal article
- Language
- en
- Open access
- green
- ruj.uj.edu.pl
- Licence
- Locations
- Cell Biochemistry and Function (publishedVersion)
- PubMed (publishedVersion)
- Jagiellonian University Repository (Jagiellonian University) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Keywords
- allyl sulfides, diallyl trisulfide, sulfane sulfur, hepatoblastoma, HepG2 cells, cell proliferation, caspase-3, apoptosis, hydrogen peroxide, thiol content, glutathione, cysteine, cysteinylglycine, rhodanese, 3-mercaptopyruvate sulfurtransferase, cystathionine gamma-lyase, bioreduction
- MeSH terms
- Anaerobiosis, Cell Survival, Garlic, Humans, Liver Neoplasms, Plant Extracts, Sulfhydryl Compounds, Sulfur, Sulfur Compounds, Hepatoblastoma, Cell Proliferation, Caspase 3, Hep G2 Cells
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