Cited by 299Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsAdvanced Biosensing Techniques and Applications

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A Review on Quantitative Multiplexed Proteomics

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Pappireddi, Nishant, Martin, Lance, Wühr, Martin·2019·ChemBioChem

DOI 10.1002/cbic.201800650PMC PMC6520187

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Abstract

Over the last few decades, mass spectrometry‐based proteomics has become an increasingly powerful tool that is now able to routinely detect and quantify thousands of proteins. A major advance for global protein quantification was the introduction of isobaric tags, which, in a single experiment, enabled the global quantification of proteins across multiple samples. Herein, these methods are referred to as multiplexed proteomics. The principles, advantages, and drawbacks of various multiplexed proteomics techniques are discussed and compared with alternative approaches. We also discuss how the emerging combination of multiplexing with targeted proteomics might enable the reliable and high‐quality quantification of very low abundance proteins across multiple conditions. Lastly, we suggest that fusing multiplexed proteomics with data‐independent acquisition approaches might enable the comparison of hundreds of different samples without missing values, while maintaining the superb measurement precision and accuracy obtainable with isobaric tag quantification.

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Authors: Pappireddi, N., Martin, L., & Wühr, M.
Affiliations: Princeton University (US)
Corresponding author: Wühr, Martin
ORCID: Wühr, Martin
Venue: ChemBioChem
Publisher: Wiley
Published: 2019-04-18
Volume: 20
Issue: 10
Pages: 1210-1224
ISSN: 1439-4227 (ISSN-L)
1439-7633
DOI: 10.1002/cbic.201800650
PMC: PMC6520187
OpenAlex: W2908241150
Cited by: 299
References: 106
Citations by year: 2026: 20 · 2025: 32 · 2024: 44 · 2023: 53 · 2022: 56 · 2021: 53 · 2020: 34 · 2019: 6
FWCI: 3.84
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: onlinelibrary.wiley.com/termsAndConditions#am (am, 261 d)
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: ChemBioChem (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders: National Institute of General Medical Sciences (1R35GM128813, T32GM007388)
U.S. Department of Energy (DE-SC0018420, DE-SC0018260)
Topics: Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
Keywords: multiplexed proteomics, quantitative proteomics, isobaric tags, targeted proteomics, low-abundance proteins, data-independent acquisition, mass spectrometry
MeSH terms: Humans, Isotope Labeling, Proteins, Proteome, Proteomics, Tandem Mass Spectrometry

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