Cited by 280Hypothalamic control of reproductive hormonesNeuropeptides and Animal PhysiologyNeuroendocrine regulation and behavior

Coexpression of dynorphin and neurokinin B immunoreactivity in the rat hypothalamus: morphologic evidence of interrelated function within the arcuate nucleus

Burke, Michelle C., Letts, Penny A., Krajewski, Sally J., Rance, Naomi E.·2006·Journal of Comparative Neurology

Abstract

Considerable evidence suggests that dynorphin and neurokinin B (NKB) neurons in the hypothalamic arcuate nucleus participate in the sex-steroid regulation of reproduction. In the present study, we used dual-label immunofluorescence to explore the distribution of prodynorphin and proNKB immunoreactivity in the rat hypothalamus. Additionally, we investigated whether arcuate prodynorphin-ir (immunoreactive) neurons expressed the neurokinin 3 receptor (NK3R) or nuclear estrogen receptor-alpha (ERalpha). We found that the majority of prodynorphin-ir neurons in the rat arcuate nucleus expressed proNKB, whereas nearly all (99%) of the proNKB neurons were immunoreactive for prodynorphin. The arcuate nucleus was the only site in the hypothalamus where neuronal somata coexpressing prodynorphin and proNKB-immunoreactivity were identified. A dense plexus of double-labeled prodynorphin/proNKB-ir fibers was found within the arcuate nucleus extending to the median eminence and throughout the periventricular zone of the hypothalamus. Prodynorphin/proNKB fibers were also identified in the paraventricular nucleus, anterior hypothalamic area, medial preoptic area, median preoptic nucleus, anteroventral periventricular nucleus, and bed nucleus of the stria terminalis in a distribution consistent with previously described arcuate nucleus projections. Interestingly, the majority of prodynorphin-ir neurons in the arcuate nucleus expressed NK3R, and nearly 100% of the prodynorphin-ir neurons contained nuclear ERalpha. Our results suggest that there is a close functional relationship between dynorphin and NKB peptides within the arcuate nucleus of the rat, which may include an autofeedback loop mediated through NK3R. The diverse hypothalamic projections of fibers expressing both prodynorphin and proNKB provide evidence that these neurons may participate in a variety of homeostatic and neuroendocrine processes.

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Crossref · 10.1002/cne.21086

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Authors
Burke, M. C., Letts, P. A., Krajewski, S. J., & Rance, N. E.
Affiliations
University of Arizona (US)
Corresponding author
Rance, Naomi E.
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
2006-08-17
Volume
498
Issue
5
Pages
712-726
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
OpenAlex
W1977946309
Cited by
280
References
57
Citations by year
2026: 3 · 2025: 10 · 2024: 9 · 2023: 8 · 2022: 9 · 2021: 22 · 2020: 10 · 2019: 13 · 2018: 23 · 2017: 16 · 2016: 19 · 2015: 19 · 2014: 24 · 2013: 21 · 2012: 24
FWCI
6.69
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute on Aging (AG09214, T32AG007434)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
Keywords
dynorphin, neurokinin B, prodynorphin, arcuate nucleus, hypothalamus, neurokinin-3 receptor, estrogen receptor alpha, hypothalamic projections, median eminence, paraventricular nucleus, bed nucleus of the stria terminalis, neuroendocrine regulation
MeSH terms
Animals, Arcuate Nucleus of Hypothalamus, Cell Count, Enkephalins, Female, Immunohistochemistry, Neural Pathways, Neurons, Protein Precursors, Tissue Distribution, Neurokinin B, Rats, Sprague-Dawley, Receptors, Tachykinin, Rats

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