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Cited by 439Medical and Biological SciencesMemory and Neural MechanismsNeuroscience and Neuropharmacology Research

The diencephalon of the albino rat: studies on the brain of the rat. No. 2

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Gurdjian, E. S.·1927·Journal of Comparative Neurology
DOI 10.1002/cne.900430102
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Abstract

Place in a solution of 3 to 5 per cent chloral hydrate in 95 per cent alcohol f o r five days (three changes).Treat with silver nitrate the same as in method I. Reduce either in the above-mentioned reducing mixture or-Pyrogallic acid, 3 Hydroquinone, 2 Formalin, 5 Water, 100for three to five days (three changes).Dehydrate and embed as in method I.Method 111.This method has given u s quite constantly certain differential staining changes which are very helpful if conservatively used (in the present paper the proper course of the medial amygdalo-hypothalamic tract was worked out in material prepared according to this method).Fix in-Concentrated ammonia, 1 cc.Chloral hydrate, 3 grams 95 per cent alcohol, 100 ec.for five days (three changes).Place in 1 to 14 per cent silver-nitrate solution for seven to ten.days as in method I. Reduce in the reducing mixture mentioned under method 11.This is very important.Method IV.This method is employed where it is desired to impregnate large pieces.I f possible to inject the material, this is done.This method is essentially the same as the method I, except that the material is left in the fixing fluid for ten to twenty days, in the silver solution (0.75 per cent) for two to four weeks, if necessary.For the silver reduction the material is left in the reducing fluid for a week to ten days and longer, if necessary.

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  1. UROUWER, B. 1917 Uber die Sehstrahlung des hfenschen. Monatsch. fur Psy- CAJAL, S. R. Y 1911 Histologie (In systkine nerveux, vol. 2. CRAIGIE, E. 11. 1925 Finer anatonry of the central nervous system based upon
  2. T H E JOC'RNkL O F COXP4RdTIVE NFVROLOC>Y, VOL. 43, NO. 1
  3. I,EWANDOWS KY, M. 190% Truncus cerebri. Janaiscbe Dcnksch., Bd. 10. Arb. Neur. Inst. Wiener Universitat, Bd. 10. Jour. Comp. Neur., vol'. 20. New York. Jour. Comp. Neur., vol. 28. 1923 Jour. Comp. Neur., vol. 35.
  4. R M I T I ~, P. 1926 Ablation and transplantation of the hypophysis i n the rat. Anat. Ree., vol. 32.

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Authors
Gurdjian, E. S.
Affiliations
University of Michigan (US)
Corresponding author
Gurdjian, E. S.
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
1927-04
Volume
43
Issue
1
Pages
1-114
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
DOI
10.1002/cne.900430102
OpenAlex
W2012600555
Cited by
439
References
23
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 7 · 2019: 1 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 4
FWCI
10.87
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 28315 d)
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • Deep Blue (University of Michigan) (acceptedVersion)
Topics
  • Health Sciences › Medicine › Anatomy › Medical and Biological Sciences
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
diencephalon, silver staining, silver nitrate impregnation, pyrogallol, formalin fixation, brain histology

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