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Cited by 228Neuroscience and Neuropharmacology ResearchNeuroendocrine regulation and behaviorRegulation of Appetite and Obesity

The magnocellular and parvocellular paraventricular nucleus of rat: intrinsic organization

Van Den Pol, Anthony N.·1982·Journal of Comparative Neurology
DOI 10.1002/cne.902060402
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Abstract

The magnocellular and paravocellular regions of the rat hypothalamic paraventricular nucleus (PVN) were examined in several hundred brains. Converging qualitative and quantitative anatomical methods, including Golgi impregnations, Nissl stains, silver stains, and immunocytochemistry were used to study the intrinsic organization of the PVN with light, scanning, and transmission electron microscopy. A computer-assisted quantitative analysis of dendritic branching patterns was used to examine total dendritic length, center of mass, orientation of dendritic tree, and several other parameters of dendritic organization and revealed statistically significant differences between cells in the lateral and posterolateral magnocellular and medial parvocellular areas of PVN. Electron microscopy, Golgi impregnation, and neurophysin immunohistochemistry showed that dendrites of posterolateral cells were generally oriented perpendicular to the third ventricle; dendrites of cells in the lateral PVN usually projected medially from the perikaryon. Cells in the medial zone of PVN had dendritic trees which often paralleled the third ventricle. Large numbers of axons entered and left PVN ventrally near the midline and laterally in the area of the posterolateral PVN; axons generally were oriented parallel to the mean major axis of dendritic trees in these areas. Ultrastructural examination of serial thin sections showed a peculiar astroglia multiple lamellar isolation of axodendritic synaptic contacts. Intrinsic axons commonly arose from parvocellular but not from magnocellular neurons and contacted dendrites of both medial parvocellular and more lateral magnocellular neurons. Synapses were found on shafts and spines of dendrites, on perikarya and somatic appendages, and invaginated into the soma. Both dendrites axons with large neurosecretory vesicles and immunostained with neurophysin antiserum were found postsynaptic to other axons. Presynaptic neurosecretory axons were not found within the PVN. A semiquantitative analysis of catecholamine axons identified with the glyoxylic acid method and fibers immunoreactive with ACTH and Substance P antisera indicated that the parvocellular region of PVN received ggreater innervation than the lateral magnocellular area; similarly, a reater density of stained fibers was found in the medial parvocellular PVN region with Golgi impregnations and silver stains. With a stereological analysis of 1-micrometer plastic sections, the parvocellular area had a significantly greater neuropil to cell volume ration, with cells accounting for 48 +/- 9% in the lateral magnocellular zone, but only for 26 +/- 7% in the parvocellular area. A quantitative analysis of vasculature from thin sections showed that the PVN had 3.3 times more blood vessels, and 3.6 times more lumen perimeter than a control area ventrolateral to PVN; an interesting finding here was that the medial parvocellular PVN had a high degree of vascularity, not significantly different from the lateral magnocellular zone...

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Research works printed but not linked: 9

  1. Armstrong W. E.(1979)Organization of Projection Neurons in the Rat Hypothalamic Paraventricular Nucleus. Doctoral Dissertation Michigan State University.
  2. Barry J., 1975, Essai de classification, en technique de Golgi, des diverses categories de neurones du noyau paraventriculaire chez la Souris, C. R. Soc. Biol. (Paris), 169, 978
  3. Buijs R. M., 1980, A Light‐ and Electron Microscope Study
  4. Christ J. F., 1966, The Pituitary. Berkeley, 62
  5. Hatton G. I., 1981, Recurrent inhibition and phasic bursting in rat paraventricular neurons: A challenge from the hypothalamic in vitro slice, Neurosci. Abstr., 7, 324
  6. Koh E. T.(1981)Anatomical Evidence of a Visceral Map Within the Paraventricular Nucleus of the Hypothalamus. Doctoral Dissertation. Harvard University.
  7. Renaud L. P., 1978, The Hypothalamus, 269
  8. Sternberger L., 1979, Immunocytochemistry
  9. van den Pol A. N., 1978, A Golgi and EM study of the rat hypothalamic magnocellular paraventricular nucleus, Soc. Neurosci. Abstr., 4, 357

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Authors
Van Den Pol, A. N.
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
1982-04-20
Volume
206
Issue
4
Pages
317-345
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
DOI
10.1002/cne.902060402
OpenAlex
W2015442553
Semantic Scholar
1b514835b0d3dd296f6f578870cb1eeca391883f
Cited by
228
References
72
Citations by year
2026: 3 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 5 · 2017: 2 · 2016: 4 · 2015: 9 · 2014: 6 · 2013: 10 · 2012: 1
FWCI
5.18
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 8208 d)
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
Keywords
magnocellular neurons, parvocellular neurons, dendrite orientation, axodendritic synapses, neurosecretory axons, neurophysin, catecholamine innervation, ACTH, substance P, hypothalamus, third ventricle, stereology, rat brain
MeSH terms
Animals, Axons, Cell Communication, Dendrites, Hypothalamus, Microscopy, Electron, Neurons, Neurophysins, Optic Chiasm, Oxytocin, Paraventricular Hypothalamic Nucleus, Rats, Inbred F344, Supraoptic Nucleus, Synapses, Synaptic Membranes, Synaptic Vesicles, Vasopressins, Rats

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