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Cited by 278

The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.

Vibrational spectroscopy—A powerful tool for the rapid identification of microbial cells at the single‐cell level

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Harz, M., Rösch, P., Popp, J.·2009·Cytometry Part A

Abstract

Rapid microbial detection and identification with a high grade of sensitivity and selectivity is a great and challenging issue in many fields, primarily in clinical diagnosis, pharmaceutical, or food processing technology. The tedious and time‐consuming processes of current microbiological approaches call for faster ideally on‐line identification techniques. The vibrational spectroscopic techniques IR absorption and Raman spectroscopy are noninvasive methods yielding molecular fingerprint information; thus, allowing for a fast and reliable analysis of complex biological systems such as bacterial or yeast cells. In this short review, we discuss recent vibrational spectroscopic advances in microbial identification of yeast and bacterial cells for bulk environment and single‐cell analysis. IR absorption spectroscopy enables a bulk analysis whereas micro‐Raman‐spectroscopy with excitation in the near infrared or visible range has the potential for the analysis of single bacterial and yeast cells. The inherently weak Raman signal can be increased up to several orders of magnitude by applying Raman signal enhancement methods such as UV‐resonance Raman spectroscopy with excitation in the deep UV region, surface enhanced Raman scattering, or tip‐enhanced Raman scattering. © 2008 International Society for Advancement of Cytometry

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Metadata

Authors
Harz, M., Rösch, P., & Popp, J.
Venue
Cytometry Part A
Publisher
Wiley
Published
2009-01-20
Volume
75A
Issue
2
Pages
104-113
ISSN
  • 1552-4922 (ISSN-L)
  • 1552-4930
OpenAlex
W2079818745
Cited by
278
References
67
FWCI
13.12
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Vibrational spectroscopy—A powerful tool for the rapid identification of microbial cells at the single‐cell level. https://app.cochat.ai/papers/10.1002/cyto.a.20682
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Harz, M., Rösch, P., & Popp, J. (2009). Vibrational spectroscopy—A powerful tool for the rapid identification of microbial cells at the single‐cell level. Cytometry Part A. https://doi.org/10.1002/cyto.a.20682

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