Cited by 193Neurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene RegulationCongenital heart defects research

Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis

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Kawaguchi, Ayano, Ikawa, Tomoko, Kasukawa, Takeya, Ueda, Hiroki R., Kurimoto, Kazuki, Saitou, Mitinori, Matsuzaki, Fumio·2008·Development

Abstract

Cellular diversity of the brain is largely attributed to the spatial and temporal heterogeneity of progenitor cells. In mammalian cerebral development,it has been difficult to determine how heterogeneous the neural progenitor cells are, owing to dynamic changes in their nuclear position and gene expression. To address this issue, we systematically analyzed the cDNA profiles of a large number of single progenitor cells at the mid-embryonic stage in mouse. By cluster analysis and in situ hybridization, we have identified a set of genes that distinguishes between the apical and basal progenitors. Despite their relatively homogeneous global gene expression profiles, the apical progenitors exhibit highly variable expression patterns of Notch signaling components, raising the possibility that this causes the heterogeneous division patterns of these cells. Furthermore, we successfully captured the nascent state of basal progenitor cells. These cells are generated shortly after birth from the division of the apical progenitors, and show strong expression of the major Notch ligand delta-like 1, which soon fades away as the cells migrate in the ventricular zone. We also demonstrated that attenuation of Notch signals immediately induces differentiation of apical progenitors into nascent basal progenitors. Thus, a Notch-dependent feedback loop is likely to be in operation to maintain both progenitor populations.

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  • Modules G2 and G3 are enriched in SZi, and G3 is enriched for genes marking Svet1+ IPs in E14 mouse SZ.

    “Modules G2 and G3 are enriched in the SZi (Fig. 3d, white bars in bottom two rows), and G3 is enriched for genes marking Svet1+ IPs in E14 mouse SZ43, including ELAVL4, LRP8, NEUROD1, NRN1, SLC17A6, SSTR2, and TP53INP1.”

    From Transcriptional landscape of the prenatal human brain (2014) · cites this paper as [43]

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Authors
Kawaguchi, A., Ikawa, T., Kasukawa, T., Ueda, H. R., Kurimoto, K., Saitou, M., & Matsuzaki, F.
Affiliations
  • RIKEN (JP)
  • Japan Science and Technology Agency (JP)
Corresponding author
  • Kawaguchi, Ayano
  • Matsuzaki, Fumio
Venue
Development
Published
2008-09-15
Volume
135
Issue
18
Pages
3113-3124
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W2141932458
Cited by
193
References
40
Citations by year
2026: 6 · 2025: 3 · 2024: 7 · 2023: 4 · 2022: 4 · 2021: 13 · 2020: 13 · 2019: 9 · 2018: 9 · 2017: 12 · 2016: 10 · 2015: 12 · 2014: 13 · 2013: 15 · 2012: 13
FWCI
5.08
Type
journal article
Language
en
Open access
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology (CREST)
  • Japan Society for the Promotion of Science (18680030, 15700264, CREST)
  • Japan Science and Technology Agency (CREST)
  • Core Research for Evolutional Science and Technology
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
Keywords
neural progenitor cells, apical progenitors, basal progenitors, Notch signaling, Delta-like 1, Notch ligands, progenitor heterogeneity, brain development, progenitor cell differentiation, single-cell gene expression profiling
MeSH terms
Animals, Embryo, Mammalian, Immunohistochemistry, Mammals, Mice, Inbred Strains, Nervous System, Neurons, Stem Cells, Signal Transduction, Biomarkers, Cluster Analysis, Prosencephalon, In Situ Hybridization, DNA, Complementary, Gene Expression Regulation, Developmental, Cell Lineage, Gene Expression Profiling, Organogenesis, Mice, Receptors, Notch

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