Cited by 141Retinal Development and DisordersNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

Notch signaling regulates regeneration in the avian retina

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Hayes, Susan, Nelson, Branden R., Buckingham, Brian, Reh, Thomas A.·2007·Developmental Biology

Abstract

The chicken retina is capable of limited regeneration. In response to injury, some Müller glia proliferate and de-differentiate into progenitor cells. However, most of these progenitors fail to differentiate into neurons. The Notch pathway is upregulated during retinal regeneration in both fish and amphibians. Since the Notch signaling pathway maintains cells in a progenitor state during development, we hypothesized that a persistently active Notch pathway might prevent a more successful regeneration in the chick retina. We found that Notch signaling components are upregulated in the proliferating progenitors. We also found that blocking the Notch pathway while Müller glia are de-differentiating into progenitor cells prohibits regeneration; conversely, blocking the Notch pathway after the progenitors have been generated from the Müller glia caused a significant increase in the percentage of new neurons. Thus, Notch signaling appears to play two distinct roles during retinal regeneration. Initially, Notch activity is necessary for the de-differentiation/proliferation of Müller glia, while later it inhibits the differentiation of the newly generated progenitor cells.

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Crossref · 10.1016/j.ydbio.2007.09.046

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Authors
Hayes, S., Nelson, B. R., Buckingham, B., & Reh, T. A.
Affiliations
University of Washington (US)
Corresponding author
Reh, Thomas A.
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2007-12
Volume
312
Issue
1
Pages
300-311
ISSN
0012-1606 (ISSN-L)
OpenAlex
W2152602928
Cited by
141
References
45
Citations by year
2026: 5 · 2025: 5 · 2024: 6 · 2023: 9 · 2022: 6 · 2021: 8 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 11 · 2015: 13 · 2014: 7 · 2013: 8 · 2012: 9
FWCI
3.31
Type
journal article
Language
en
Open access
Locations
  • Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (EY13475)
  • Israel National Road Safety Authority (F32 EY016636)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
Notch signaling, retinal regeneration, Müller cells, progenitor cells, dedifferentiation, neuronal differentiation, chicken retina, avian retina, retinal damage
MeSH terms
Animals, Cell Count, Cell Differentiation, Chickens, gamma-Aminobutyric Acid, Neuroglia, Neurons, Regeneration, Repressor Proteins, Retina, Stem Cells, Triglycerides, Signal Transduction, Down-Regulation, Up-Regulation, Cell Proliferation, Receptors, Notch

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