The DOI matches its Crossref record.
Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension
Abstract
We describe a modification in the sample application mode for isoelectric focusing with immobilized pH gradients. Instead of being applied at the surface of the gel in a sample cup, the sample is introduced into the gel during the immobilized pH gradient strip rehydration step. This modification implies the use of low percentage gels (below 3.5%T) and specially designed, but simple, rehydration chambers. The main advantages are a uniform resolution without side effects and the possibility of handling large sample volumes (500 μL for a standard 3 × 160 × 0.5 mm strip), allowing micropreparative work (milligram samples) with a simple experimental design.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Proteomics Analysis of Cellular Response to Oxidative Stress
Rabilloud, T., Heller, M., Gasnier, F., Luche, S., Rey, C., Aebersold, R., Benahmed, M., Louisot, P., & Lunardi, J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m106585200 · Cited by 393
Self-citationPeltier, J.-B., Friso, G., Kalume, D. E., Roepstorff, P., Nilsson, F., Adamska, I., & van Wijka, K. J. (2000) · The Plant Cell · doi:10.1105/tpc.12.3.319 · Cited by 355
Kawada, N., Kristensen, D. B., Asahina, K., Nakatani, K., Minamiyama, Y., Seki, S., & Yoshizato, K. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102630200 · Cited by 339
Use of thiourea to increase the solubility of membrane proteins in two‐dimensional electrophoresis
Rabilloud, T. (1998) · ELECTROPHORESIS · doi:10.1002/elps.1150190526 · Cited by 337
Self-citationChevallet, M., Santoni, V., Poinas, A., Rouquié, D., Fuchs, A., Kieffer, S., Kieffer, S., Lunardi, J., Garin, J., & Rabilloud, T. (1998) · ELECTROPHORESIS · doi:10.1002/elps.1150191108 · Cited by 331
Self-citation
Metadata
- Authors
- Rabilloud, T., Valette, C., & Lawrence, J. J.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1994-01
- Volume
- 15
- Issue
- 1
- Pages
- 1552-1558
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2018571987
- Cited by
- 366
- References
- 0
- FWCI
- 6.61
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension. https://app.cochat.ai/papers/10.1002/elps.11501501223
- The paper
- Rabilloud, T., Valette, C., & Lawrence, J. J. (1994). Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension. ELECTROPHORESIS. https://doi.org/10.1002/elps.11501501223
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
