Advances in process monitoring tools for cell culture bioprocesses
Abstract
The productivity of cell culture manufacturing for biologics has increased momentously in the past decades. Increasingly, the process research efforts are devoted into improving product quality and consistency. Consistent process performance and successful implementation of quality by design practice requires well‐utilized process analytical technology. This review summarizes recent progress and current status of bioprocess monitoring. Many sensors for bioprocess monitoring have been available for decades while new ones, especially spectrometric sensors, are making their way into cell culture bioprocesses. On‐line sampling devices have grown mature in the past decade thus making many instruments traditionally used for off‐line analysis available for at‐line use. With a general trend of using better defined medium for cell cultivation and increasing emphasis of process analytical tools, the spectrometric methods are also making headway in cell culture process monitoring. The integration of those sensing technologies will be important to advance the real‐time monitoring of the state of cellular physiology for the control for process consistency and product quality.
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MIR, NIR, and Raman vibrational spectroscopies are promising techniques to monitor multiple molecular analytes in any biological processes on-line/in-line.
“Overall, MIR, NIR, and Raman vibrational spectroscopies are promising techniques to monitor multiple molecular analytes in any biological processes on-line/in-line [31].”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [31]
On-line chemometric calibration models of NIR spectra of macromolecules in biotechnology can be considered one of the most complex applications of chemometrics in process monitoring.
“On the other hand, on-line chemometric calibration models of NIR spectra of macromolecules in biotechnology can be considered one of the most complex applications of chemometrics in process monitoring [31].”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [31]
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- Authors
- Zhao, L., Fu, H., Zhou, W., & Hu, W.
- Affiliations
- University of Minnesota (US)
- WuXi AppTec (China) (CN)
- Corresponding author
- Hu, Wei‐Shou
- Venue
- Engineering in Life Sciences
- Publisher
- Wiley
- Published
- 2015-04-24
- Volume
- 15
- Issue
- 5
- Pages
- 459-468
- ISSN
- 1618-0240 (ISSN-L)
- 1618-2863
- OpenAlex
- W1967102319
- Cited by
- 81
- References
- 74
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 6 · 2023: 8 · 2022: 8 · 2021: 10 · 2020: 6 · 2019: 9 · 2018: 10 · 2017: 6 · 2016: 7 · 2015: 2
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Engineering in Life Sciences (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Engineering › Control and Systems Engineering › Fault Detection and Control Systems
- Keywords
- process monitoring, process analytical technology, quality by design, biopharmaceutical manufacturing, real-time monitoring, cell physiology, process consistency, product quality
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