Ultrasound-Enhanced Attenuated Total Reflection Mid-infrared Spectroscopy In-Line Probe: Acquisition of Cell Spectra in a Bioreactor
Open accessAbstract
This article presents a novel method for selective acquisition of Fourier transform infrared (FT-IR) spectra of microorganisms in-line during fermentation, using Saccharomyces cerevisiae as an example. The position of the cells relative to the sensitive region of the attenuated total reflection (ATR) FT-IR probe was controlled by combing a commercially available ATR in-line probe with contact-free, gentle particle manipulation by ultrasonic standing waves. A prototype probe was successfully constructed, assembled, and tested in-line during fed-batch fermentations of S. cerevisiae. Control over the position of the cells was achieved by tuning the ultrasound frequency: 2.41 MHz was used for acquisition of spectra of the cells (pushing frequency fp) and 1.87 MHz, for retracting the cells from the ATR element, therefore allowing spectra of the medium to be acquired. Accumulation of storage carbohydrates (trehalose and glycogen) inside the cells was induced by a lack of a nitrogen source in the feed medium. These changes in biochemical composition were visible in the spectra of the cells recorded in-line during the application of fp and could be verified by reference spectra of dried cell samples recorded off-line with a FT-IR microscope. Comparison of the cell spectra with spectra of trehalose, glycogen, glucose, and mannan, i.e., the major carbohydrates present in S. cerevisiae, and principal components analysis revealed that the changes observed in the cell spectra correlated well with the bands specific for trehalose and glycogen. This proves the applicability and capability of ultrasound-enhanced in-line ATR mid-IR spectroscopy as a real-time PAT method for the in situ monitoring of cellular biochemistry during fermentation.
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Most applications of vibrational spectroscopy PAT require calibration and validation of chemometric models, with the exception of some analytes such as carotenoids measured through Raman.
“Most applications of vibrational spectroscopy PAT require calibration and validation of chemometric models [21, 48,102] with the exception of some analytes, for example carotenoids measured through Raman [94].”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [94]
The introductory passage (labeled Abstract in our copy; page unavailable) says chemometrics can extract chemical information from spectroscopic data, and the Data Analysis section describes using PCA. The paper does not establish that most vibrational-spectroscopy PAT applications require calibration and validation of chemometric models. The carotenoid exception is attached to a different citation in the citing sentence. (LLM, conf=0.81)
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Sorted by citations
Advances in process monitoring tools for cell culture bioprocesses
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- Authors
- Koch, C., Brandstetter, M., Wechselberger, P., Lorantfy, B., Plata, M. R., Radel, S., Herwig, C., & Lendl, B.
- Affiliations
- TU Wien (AT)
- Vienna University of Technology , , Gumpendorferstraße 1a , ,
- Vienna University of Technology , , Getreidemarkt 9/164-UPA , ,
- Corresponding author
- Lendl, Bernhard
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-01-12
- Volume
- 87
- Issue
- 4
- Pages
- 2314-2320
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- PMC
- PMC4333607
- OpenAlex
- W2095503753
- Cited by
- 35
- References
- 46
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 4 · 2022: 2 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 1
- FWCI
- 2.02
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Analytical Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Chalmers Publication Library (Chalmers University of Technology) (submittedVersion)
- Chalmers Research (Chalmers University of Technology) (submittedVersion)
- Funders
- Austrian Science Fund (P24154-N17)
- Österreichische Forschungsförderungsgesellschaft (825340)
- Fakultät für Technische Chemie, Technische Universität Wien (NA)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Chemistry › Spectroscopy › Spectroscopy and Laser Applications
- Keywords
- Saccharomyces cerevisiae, fed-batch fermentation, process analytical technology, trehalose, glycogen, storage carbohydrates, nitrogen starvation, principal component analysis, real-time fermentation monitoring
- MeSH terms
- Fermentation, Saccharomyces cerevisiae, Ultrasonics, Molecular Probes, Spectroscopy, Fourier Transform Infrared, Bioreactors
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