A Deep Dive into the Complex Chemical Mixture and Toxicity of Tire Wear Particle Leachate in Fathead Minnow
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The ecological impact of tire wear particles in aquatic ecosystems is a growing environmental concern. We combined toxicity testing, using fathead minnow (Pimephales promelas) embryos, with nontarget high-resolution liquid chromatography Orbitrap mass spectrometry to characterize the toxicity and chemical mixture of organic chemicals associated with tire particle leachates. We assessed: 1) exposure to tire particle leachates after leaching for 1-, 3-, and 10-d; and 2) the effect of the presence and absence of small tire particulates in the leachates. We observed a decrease in embryonic heart rates, hatching success, and lengths, as well as an increase in the number of embryos with severe deformities and diminished eye and body pigmentation, after exposure to the leachates. Overall, there was a pattern whereby we observed more toxicity in the 10-d leachates, and greater toxicity in unfiltered leachates. Redundancy analysis showed that several benzothiazoles and aryl-amines were correlated with the toxic effects observed in the embryos. These included benzothiazole, 2-aminobenzothiazole, 2-mercaptobenzothiazole, N,N′-diphenylguanidine, and N,N′-diphenylurea. However, many other chemicals characterized as unknowns are likely to also play a key role in the adverse effects observed. Our study provides insight into the types of chemicals likely to be important toxicological drivers in tire leachates, and improves our understanding of the ecotoxicological impacts of tire wear particles. Environ Toxicol Chem 2022;41:1144–1153. © 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Abstract Redundancy analysis (RDA) shows positive correlations between chemicals benzothiazoles and aryl-amines in tire leachates, and increased hatch severity and lack of pigmentation in fathead minnow embryos. OHBT = hydroxybenzothiazole; PBI = 3-phenyl-1,3-benzothiazol-2-imine; NHBT = 2-aminobenzothiazole; MeSBT = 2-(methylthio)benzothiazole; MBT = 2-mercaptobenzothiazole; DPG = N,N′-diphenylguanidine; CPU = cyclohexyl-3-phenylurea; DHU = N,N′-dicyclohexylurea; DPU = N,N′-diphenylurea; DHPA = 4,5-diphenyl-1H-pyrazole-3-amine; DHA = dicyclohexylamine; HM = hexa(methoxymethyl)melamine; ABZ = 3-aminobenzamide; BT = benzothiazole; ACT = acetoacetanilide; TMQ = 1,2-Dihydro-2,2,4-trimethylquinoline; ACR = acridene.
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Laboratory experiments using fish have found that microplastics can cause deformities during development.
“and deformities during development.27”
From Microplastics and Anthropogenic Particles in Recreationally Caught Freshwater Fish from an Urbanized Region of the North American Great Lakes (2024) · cites this paper as [27]
Tire rubber microparticles have been demonstrated to have harmful effects on fish.
“owing to the harmful effects that tire rubber microparticles have been demonstrated to have on fish.”
From Microplastics and Anthropogenic Particles in Recreationally Caught Freshwater Fish from an Urbanized Region of the North American Great Lakes (2024) · cites this paper as [27]
Fish exposed to tire particles and their leachate have been observed to show altered behavior and growth, as well as decreased heart rates and reduced hatching success.
“fish exposed to tire particles and their leachate have been observed to show altered behavior and growth,26 as well as decreased heart rates and reduced hatching success.27”
From Microplastics and Anthropogenic Particles in Recreationally Caught Freshwater Fish from an Urbanized Region of the North American Great Lakes (2024) · cites this paper as [27]
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- Authors
- Chibwe, L., Parrott, J. L., Shires, K., Khan, H., Clarence, S., Lavalle, C., Sullivan, C., O'Brien, A. M., De Silva, A. O., Muir, D. C. G., & Rochman, C. M.
- Affiliations
- University of Toronto (CA)
- Environment and Climate Change Canada (CA)
- Aquatic Contaminants Research Division Environment & Climate Change Canada, Burlington Ontario Canada
- Corresponding author
- Chibwe, Leah
- Rochman, Chelsea M.
- ORCID
- Venue
- Environmental Toxicology and Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2021-06-14
- Volume
- 41
- Issue
- 5
- Pages
- 1144-1153
- ISSN
- 0730-7268 (ISSN-L)
- 1552-8618
- PMC
- PMC9291566
- OpenAlex
- W3170459253
- Cited by
- 159
- References
- 59
- Citations by year
- 2026: 27 · 2025: 48 · 2024: 46 · 2023: 22 · 2022: 12 · 2021: 4
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Environmental Toxicology and Chemistry (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Keywords
- tire wear particles, combined toxicity, fathead minnow, embryotoxicity, benzothiazole, aromatic amines, N,N'-diphenylurea, embryonic malformations, pigmentation loss, aquatic ecotoxicology
- MeSH terms
- Animals, Cyprinidae, Water Pollutants, Chemical, Ecosystem, Toxicity Tests, Ecotoxicology
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