The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Novel NG2‐CreERT2 knock‐in mice demonstrate heterogeneous differentiation potential of NG2 glia during development
Open accessAbstract
NG2 (nerve/glia antigen‐2) is a type I transmembrane glycoprotein and also known as chondroitin sulfate proteoglycan 4. In the parenchyma of the central nervous system, NG2‐expressing (NG2+) cells have been identified as a novel type of glia with a strong potential to generate oligodendrocytes (OLs) in the developing white matter. However, the differentiation potential of NG2 glia remained controversial, largely attributable to shortcomings of transgenic mouse models used for fate mapping. To minimize these restrictions and to more faithfully mimic the endogenous NG2 expression in vivo, we generated a mouse line in which the open reading frame of the tamoxifen‐inducible form of the Cre DNA recombinase (CreERT2) was inserted into the NG2 locus by homologous recombination. Results from this novel mouse line demonstrate that at different developmental stages of the brain, NG2+ cells either stayed as NG2 glia or differentiated into OLs during the whole life span. Interestingly, when Cre activity was induced at embryonic stages, a significant number of reporter+ astrocytes could be detected in the gray matter after birth. However, in other brain regions, such as olfactory bulb, brain stem, and cerebellum, all of the NG2 glia was restricted to the OL lineage. In addition, tamoxifen‐sensitive and NG2 gene locus‐dependent gene recombination could be detected in a small, but persistent population of cortical NeuN+ neurons starting from the second postnatal week. GLIA 2014;62:896–913
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Citations
Cites
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain
Madisen, L., Zwingman, T. A., Sunkin, S. M., Oh, S. W., Zariwala, H. A., Gu, H., Ng, L. L., Palmiter, R. D., Hawrylycz, M. J., Jones, A. R., et al. (2010) · Nature Neuroscience · doi:10.1038/nn.2467 · Cited by 6,907
Kessaris, N., Fogarty, M., Iannarelli, P., Grist, M., Wegner, M., & Richardson, W. D. (2006) · Nature Neuroscience · doi:10.1038/nn1620 · Cited by 1,195
PDGFRA/NG2 glia generate myelinating oligodendrocytes and piriform projection neurons in adult mice
Rivers, L. E., Young, K. M., Rizzi, M., Jamen, F., Psachoulia, K., Wade, A., Kessaris, N., & Richardson, W. D. (2008) · Nature Neuroscience · doi:10.1038/nn.2220 · Cited by 961
Dawson, M. (2003) · Molecular and Cellular Neuroscience · doi:10.1016/s1044-7431(03)00210-0 · Cited by 927
Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling
Young, K. M., Psachoulia, K., Tripathi, R. B., Dunn, S.-J., Cossell, L., Attwell, D., Tohyama, K., & Richardson, W. D. (2013) · Neuron · doi:10.1016/j.neuron.2013.01.006 · Cited by 919 · cited 3×
“Recent reports using EdU (5-ethynyl-20-deoxyuridine) instead of BrdU even suggest that not only 80% but almost all NG2 glia (more than 98%) incorporated EdU, thereby reducing the portion of potential neurogenic NG2 glia (Clarke et al., 2012; Young et al., 2013).”
BackgroundMethodNeurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte–Neuron Communication
Frühbeis, C., Fröhlich, D., Kuo, W. P., Amphornrat, J., Thilemann, S., Saab, A. S., Kirchhoff, F., Möbius, W., Goebbels, S., Nave, K.-A., et al. (2013) · PLoS Biology · doi:10.1371/journal.pbio.1001604 · Cited by 902
Self-citationPolydendrocytes (NG2 cells): multifunctional cells with lineage plasticity
Nishiyama, A., Komitova, M., Suzuki, R., & Zhu, X. (2009) · Nature Reviews Neuroscience · doi:10.1038/nrn2495 · Cited by 865
Age-dependent fate and lineage restriction of single NG2 cells
Zhu, X., Hill, R. A., Dietrich, D., Komitova, M., Suzuki, R., & Nishiyama, A. (2011) · Development · doi:10.1242/dev.047951 · Cited by 464
The NG2 proteoglycan: Past insights and future prospects
Stallcup, W. B. (2002) · Journal of Neurocytology · doi:10.1023/a:1025731428581 · Cited by 296
A crucial role for Olig2 in white matter astrocyte development
Cai, J., Chen, Y., Cai, W.-H., Hurlock, E. C., Wu, H., Kernie, S. G., Parada, L. F., & Lu, Q. R. (2007) · Development · doi:10.1242/dev.02847 · Cited by 203
Cited by
Verkhratsky, A., & Nedergaard, M. (2018) · Physiological Reviews · doi:10.1152/physrev.00042.2016 · Cited by 1,678
Amyloid β oligomers constrict human capillaries in Alzheimer's disease via signaling to pericytes
Nortley, R., Korte, N., Izquierdo, P., Hirunpattarasilp, C., Mishra, A., Jaunmuktane, Z., Kyrargyri, V., Pfeiffer, T., Khennouf, L., Madry, C., et al. (2019) · Science · doi:10.1126/science.aav9518 · Cited by 682
“Experiments were carried out on acute cortical brain slices from P44 to P88 mice, of either sex, generated by crossing tamoxifen-inducible NG2Cre mice (45) with floxed GCaMP5G-IREStdTomato mice (JAX 024477).”
MethodSelf-citationSingle-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues
Bartosovic, M., Kabbe, M., & Castelo-Branco, G. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-00869-9 · Cited by 483
Lifelong cortical myelin plasticity and age-related degeneration in the live mammalian brain
Hill, R. A., Li, A. M., & Grutzendler, J. (2018) · Nature Neuroscience · doi:10.1038/s41593-018-0120-6 · Cited by 467
Fard, M. K., van der Meer, F., Sánchez, P., Cantuti-Castelvetri, L., Mandad, S., Jäkel, S., Fornasiero, E. F., Schmitt, S., Ehrlich, M., Starost, L., et al. (2017) · Science Translational Medicine · doi:10.1126/scitranslmed.aam7816 · Cited by 249
“We crossbred transgenic mice expressing a tamoxifen-inducible Cre DNA recombinase inserted into the NG2 locus [NG2-CreERT2 (22)] with a widely used reportermouse strain containing amembrane-targeted enhanced green fluorescent protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus (23).”
Method
Metadata
- Authors
- Huang, W., Zhao, N., Bai, X., Karram, K., Trotter, J., Goebbels, S., Scheller, A., & Kirchhoff, F.
- Venue
- Glia
- Publisher
- Wiley
- Published
- 2014-02-28
- Volume
- 62
- Issue
- 6
- Pages
- 896-913
- ISSN
- 0894-1491 (ISSN-L)
- 1098-1136
- OpenAlex
- W1519559613
- Cited by
- 181
- References
- 11
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Huang, W., Zhao, N., Bai, X., Karram, K., Trotter, J., Goebbels, S., Scheller, A., & Kirchhoff, F. (2014). Novel NG2‐CreERT2 knock‐in mice demonstrate heterogeneous differentiation potential of NG2 glia during development. Glia. https://doi.org/10.1002/glia.22648
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