A new class of DNA photolyases present in various organisms including aplacental mammals.
Open accessAbstract
DNA photolyase specifically repairs UV light-induced cyclobutane-type pyrimidine dimers in DNA through a light-dependent reaction mechanism. We have obtained photolyase genes from Drosophila melanogaster (fruit fly), Oryzias latipes (killifish) and the marsupial Potorous tridactylis (rat kangaroo), the first photolyase gene cloned from a mammalian species. The deduced amino acid sequences of these higher eukaryote genes show only limited homology with microbial photolyase genes. Together with the previously cloned Carassius auratus (goldfish) gene they form a separate group of photolyase genes. A new classification for photolyases comprising two distantly related groups is proposed. For functional analysis P.tridactylis photolyase was expressed and purified as glutathione S-transferase fusion protein from Escherichia coli cells. The biologically active protein contained FAD as light-absorbing cofactor, a property in common with the microbial class photolyases. Furthermore, we found in the archaebacterium Methanobacterium thermoautotrophicum a gene similar to the higher eukaryote photolyase genes, but we could not obtain evidence for the presence of a homologous gene in the human genome. Our results suggest a divergence of photolyase genes in early evolution.
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Photolyases appear to be absent or nonfunctional in placental mammals such as humans.
“Photolyases seem to be absent or nonfunctional in placental mammals like human [118, 132, 160].”
From Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair (2010) · cites this paper as [118]
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- Authors
- Yasui, A., Eker, A. P., Yasuhira, S., Yajima, H., Kobayashi, T., Takao, M., & Oikawa, A.
- Affiliations
- Tohoku University (JP)
- Venue
- The EMBO Journal
- Published
- 1994-12-01
- Volume
- 13
- Issue
- 24
- Pages
- 6143-6151
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC395594
- OpenAlex
- W1785791360
- Cited by
- 219
- References
- 39
- Citations by year
- 2026: 5 · 2025: 1 · 2024: 7 · 2023: 6 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 7 · 2016: 3 · 2014: 9 · 2013: 3 · 2012: 6
- FWCI
- 4.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- DNA photolyase, cyclobutane pyrimidine dimers, UV-induced DNA damage, FAD cofactor, Drosophila melanogaster, medaka, goldfish, Methanobacterium thermoautotrophicum, GST fusion protein
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Deoxyribodipyrimidine Photo-Lyase, Drosophila melanogaster, Escherichia coli, Humans, Macropodidae, Molecular Sequence Data, Oryzias, Phylogeny, Pigments, Biological, Recombinant Fusion Proteins, Gene Library, Methanobacterium, Sequence Homology, Amino Acid, DNA, Complementary
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