Alternative Excision Repair Pathway of UV-damaged DNA inSchizosaccharomyces pombe Operates Both in Nucleus and in Mitochondria
Open accessAbstract
The fission yeast, Schizosaccharomyces pombe, possesses a UV-damaged DNA endonuclease-dependent excision repair (UVER) pathway in addition to nucleotide excision repair pathway for UV-induced DNA damage. We examined cyclobutane pyrimidine dimer removal from the myo2 locus on the nuclear genome and the coI locus on the mitochondrial genome by the two repair pathways. While nucleotide excision repair repairs damage only on the nuclear genome, UVER efficiently removes cyclobutane pyrimidine dimers on both nuclear and mitochondrial genomes. The ectopically expressed wild type UV-damaged DNA endonuclease was localized to both nucleus and mitochondria, while modifications of N-terminal methionine codons restricted its localization to either of two organelles, suggesting an alternative usage of multiple translation initiation sites for targeting the protein to different organelles. By introducing the same mutations into the chromosomal copy of the uvde(+) gene, we selectively inactivated UVER in either the nucleus or the mitochondria. The results of UV survival experiments indicate that although UVER efficiently removes damage on the mitochondrial genome, UVER in the mitochondria hardly contributes to UV resistance of S. pombe cells. We suggest a possible UVER function in mitochondria as a backup system for other UV damage tolerance mechanisms.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Following UVB irradiation, UVDE-initiated repair results in rapid, long-patch processes, improving survival in DNA repair-deficient cells.
“Collectively, these data present consistent evidence that following UVB irradiation, UVDE-initiated repair results in rapid, long-patch processes, improving survival in DNA repair-deficient cells.”
From Modulation of UVB-induced Carcinogenesis by Activation of Alternative DNA Repair Pathways (2018) · cites this paper as [34]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe
Moreno, S., Klar, A., & Nurse, P. (1991) · Methods in Enzymology · doi:10.1016/0076-6879(91)94059-l · Cited by 3,620 · see citing works
Bootsma, D., & Hoeijmakers, J. H. J. (1996) · Trends in Genetics · doi:10.1016/0168-9525(96)81408-9 · Cited by 2,285 · see citing works
A knowledge base for predicting protein localization sites in eukaryotic cells
Nakai, K., & Kanehisa, M. I. (1992) · Genomics · doi:10.1016/s0888-7543(05)80111-9 · Cited by 1,512 · see citing works
Comparison ofSchizosaccharomyces pombeexpression systems
Forsburg, S. L. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.12.2955 · Cited by 440 · see citing works
Donahue, B. A., Yin, S., Taylor, J., Reines, D., & Hanawalt, P. C. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.18.8502 · Cited by 358 · see citing works
Todo, T., Takemori, H., Ryo, H., lhara, M., Matsunaga, T., Nikaido, O., Sato, K., & Nomura, T. (1993) · Nature · doi:10.1038/361371a0 · Cited by 298 · see citing works
Mitochondrial targeting of human DNA glycosylases for repair of oxidative DNA damage
Takao, M., Aburatani, H., Kobayashi, K., & Yasui, A. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.12.2917 · Cited by 282 · see citing works
Self-citationSvejstrup, J. Q., Wang, Z., Feave, W. J., Wu, X., Bushnell, D. A., Donahue, T. F., Friedberg, E. C., & Kornberg, R. D. (1995) · Cell · doi:10.1016/0092-8674(95)90447-6 · Cited by 278 · see citing works
Todo, T., Ryo, H., Yamamoto, K., Toh, H., Inui, T., Ayaki, H., Nomura, T., & Ikenaga, M. (1996) · Science · doi:10.1126/science.272.5258.109 · Cited by 247 · see citing works
PCR technology, current innovations
Tracy, S., & Romero, J. (1995) · Virus Research · doi:10.1016/0168-1702(95)90075-6 · Cited by 242 · see citing works
Griffin, H. G., & Griffin, A. M. (1994) · CRC Press eBooks · doi:10.1201/b12376 · Cited by 232 · see citing works
A new class of DNA photolyases present in various organisms including aplacental mammals.
Yasui, A., Eker, A. P., Yasuhira, S., Yajima, H., Kobayashi, T., Takao, M., & Oikawa, A. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06961.x · Cited by 219 · see citing works
Self-citationKitayama, C., Sugimoto, A., & Yamamoto, M. P. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.137.6.1309 · Cited by 212 · see citing works
Involvement of Flap Endonuclease 1 in Base Excision DNA Repair
Kim, K. M., Biade, S., & Matsumoto, Y. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.15.8842 · Cited by 201 · see citing works
Efficient Repair of Abasic Sites in DNA by Mitochondrial Enzymes
Pinz, K. G., & Bogenhagen, D. F. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.3.1257 · Cited by 190 · see citing works
How can the products of a single gene be localized to more than one intracellular compartment?
Danpure, C. J. (1995) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)89016-9 · Cited by 167 · see citing works
Prakash, L. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80010-6 · Cited by 155 · see citing works
Microtubules mediate mitochondrial distribution in fission yeast.
Yaffe, M. P., Harata, D., Verde, F., Eddison, M., Toda, T., & Nurse, P. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.21.11664 · Cited by 151 · see citing works
Nuclear and mitochondrial forms of human uracil-DNA glycosylase are encoded by the same gene
Slupphaug, G., Markussen, F.-H., Olosen, L. C., Aasland, R., Aarsæther, N., Bakke, O., Krokan, H. E., & Helland, D. E. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.11.2579 · Cited by 135 · see citing works
Yajima, H., Takao, M., Yasuhira, S., Zhao, J. H., Ishii, C., Inoue, H., & Yasui, A. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb07234.x · Cited by 131 · see citing works
Self-citationYonemasu, R., McCready, S. J., Murray, J. M., Osman, F., Takao, M., Yamamoto, K., Lehmann, A., & Yasui, A. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.8.1553 · Cited by 97 · see citing works
Self-citationA nuclear mutation defective in mitochondrial recombination in yeast.
Ling, F., Makishima, F., Morishima, N., & Shibata, T. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb00081.x · Cited by 85 · see citing works
Waters, R., & Moustacchi, E. (1974) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(74)90282-2 · Cited by 77 · see citing works
Carr, A. M., Sheldrick, K. S., Sheldrick, S., Murray, J. M., Al-Harithy, R. N., Watts, F. Z., & Lehmann, A. R. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.6.1345 · Cited by 76 · see citing works
Ulery, T. L., Jang, S. H., & Jaehning, J. A. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.2.1160 · Cited by 75 · see citing works
Takao, M., Yonemasu, R., Yamamoto, K., & Yasui, A. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.7.1267 · Cited by 72 · see citing works
Self-citationMolecular biology of the fission yeast
Lee, M. G. (1990) · Trends in Genetics · doi:10.1016/0168-9525(90)90211-n · Cited by 71 · see citing works
Haffter, P., & Fox, T. D. (1992) · Genetics · doi:10.1093/genetics/131.2.255 · Cited by 71 · see citing works
Kanno, S.-I., Shigenori, I., Takao, M., & Yasui, A. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.15.3096 · Cited by 66 · see citing works
Self-citationGuzder, S. N., Sung, P., Prakash, L., & Prakash, S. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.11.6292 · Cited by 64 · see citing works
An Alternative Eukaryotic DNA Excision Repair Pathway
Freyer, G. A., Davey, S. K., Ferrer, J. V., Martin, A. M., Beach, D., & Doetsch, P. W. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.8.4572 · Cited by 64 · see citing works
Fission yeast gene structure and recognition
Zhang, M. Q., & Marr, T. G. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.9.1750 · Cited by 61 · see citing works
Pyrimidine dimers block simian virus 40 replication forks.
Berger, C. A., & Edenberg, H. J. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.10.3443 · Cited by 51 · see citing works
Mitochondrial DNA repair by photolyase
Yasui, A., Yajima, H., Kobayashi, T., Eker, A. P. M., & Oikawa, A. (1992) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(92)90084-g · Cited by 47 · see citing works
Self-citationKlocker, H., Schneider, R., Hj, B., Auer, B., Hirsch‐Kauffmann, M., & Schweiger, M. R. (1986) · PubMed · Cited by 37 · see citing works
Yasuhira, S., Mitani, H., & Shima, A. (1991) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1991.tb03925.x · Cited by 35 · see citing works
Self-citationMitochondrial DNA in the fission yeast Schizosaccharomyces pombe
Bostock, C. J. (1969) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(69)90667-4 · Cited by 33 · see citing works
Yasuhira, S., Yasui, A., & Morimyo, M. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.38.26822 · Cited by 27 · see citing works
Self-citationNicolas, P., Hussein, Y., Heizmann, P., & Nigon, V. (1980) · Molecular and General Genetics MGG · doi:10.1007/bf00337862 · Cited by 25 · see citing works
Massardo, D. R., Manna, F., Schäfer, B., WOLF, K. W., & Giudice, L. D. (1994) · Current Genetics · doi:10.1007/bf00712972 · Cited by 21 · see citing works
Accommodation of pyrimidine dimers during replication of UV-damaged simian virus 40 DNA.
Stacks, P. C., White, J. H., & Dixon, K. (1983) · Molecular and Cellular Biology · doi:10.1128/mcb.3.8.1403 · Cited by 20 · see citing works
Existence and expression of photoreactivation repair genes in various yeast species
Yasui, A., Eker, A. P. M., & Koken, M. H. M. (1989) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(89)90029-3 · Cited by 12 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 2
- Yaffe, M. P., Harata, D., Verde, F., Eddison, M., Toda, T., and Nurse, P. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 11664 -11668
- Donahue, B. A., Yin, S., Taylor, J. S., Reines, D., and Hanawalt, P. C. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 8502-8506
Cited by
Sorted by citations
How nucleotide excision repair protects against cancer
Friedberg, E. C. (2001) · Nature Reviews Cancer · doi:10.1038/35094000 · Cited by 730 · see citing works
Borkovich, K. A., Alex, L. A., Yarden, O., Freitag, M., Turner, G., Read, N. D., Seiler, S., Bell‐Pedersen, D., Paietta, J. V., Plesofsky, N. S., et al. (2004) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.68.1.1-108.2004 · Cited by 626 · see citing works
Nuclear and mitochondrial DNA repair: similar pathways?
Larsen, N. B., Rasmussen, M., & Rasmussen, L. J. (2005) · Mitochondrion · doi:10.1016/j.mito.2005.02.002 · Cited by 289 · see citing works
Kaarniranta, K., Pawłowska, E., Szczepańska, J., Jabłkowska, A., & Błasiak, J. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20102374 · Cited by 193 · see citing works
DNA repair in organelles: Pathways, organization, regulation, relevance in disease and aging
Boesch, P., Weber‐Lotfi, F., Ibrahim, N., Тарасенко, В. И., Cosset, A., Paulus, F., Lightowlers, R. N., & Dietrich, A. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2010.10.002 · Cited by 165 · see citing works
Bess, A. S., Crocker, T. L., Ryde, I. T., & Meyer, J. N. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks532 · Cited by 133 · see citing works
Maintenance of mitochondrial DNA integrity: repair and degradation
Kang, D., & Hamasaki, N. (2002) · Current Genetics · doi:10.1007/s00294-002-0312-0 · Cited by 112 · see citing works
Vongsamphanh, R., Fortier, P.-K., & Ramotar, D. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.5.1647-1655.2001 · Cited by 104 · see citing works
Schäfer, B. (2003) · Current Genetics · doi:10.1007/s00294-003-0404-5 · Cited by 74 · see citing works
Alternative Excision Repair Pathways
Yasui, A. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a012617 · Cited by 59 · see citing works
Self-citationBeaudet, D., Terrat, Y., Halary, S., de la Providencia, I. E., & Hijri, M. (2013) · Genome Biology and Evolution · doi:10.1093/gbe/evt120 · Cited by 46 · see citing works
Verma, S., & Idnurm, A. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003769 · Cited by 45 · see citing works
Topoisomerase I-driven repair of UV-induced damage in NER-deficient cells
Saha, L. K., Wakasugi, M., Akter, S., Prasad, R., Wilson, S. H., Shimizu, N., Sasanuma, H., Huang, S.-Y. N., Agama, K., Pommier, Y. G., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1920165117 · Cited by 36 · see citing works
Osman, F., Bjørås, M., Alseth, I., Morland, I., McCready, S. J., Seeberg, E. C., & Tsaneva, I. R. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03440.x · Cited by 33 · see citing works
Mourrain, L., & Boissonneault, G. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222212418 · Cited by 16 · see citing works
Dinh, N., & Bonnefoy, N. (2023) · IUBMB Life · doi:10.1002/iub.2801 · Cited by 14 · see citing works
Tanihigashi, H., Yamada, A., Igawa, E., & Ikeda, S. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.06.191 · Cited by 13 · see citing works
Modulation of UVB-induced Carcinogenesis by Activation of Alternative DNA Repair Pathways
Sha, Y., Vartanian, V., Owen, N., Mengden Koon, S. J., Calkins, M. J., Thompson, C. S., Mirafzali, Z., Mir, S., Goldsmith, L. E., He, H., et al. (2018) · Scientific Reports · doi:10.1038/s41598-017-17940-8 · Cited by 12 · see citing works
Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast Schizosaccharomyces pombe
Kanamitsu, K., & Ikeda, S. (2010) · Journal of Nucleic Acids · doi:10.4061/2010/450926 · Cited by 11 · see citing works
Milo, S., Adar, S., & Covo, S. (2019) · mBio · doi:10.1128/mbio.02623-19 · Cited by 10 · see citing works
Dynamic responses of Fusarium mangiferae to ultra-violet radiation
Milo, S., Namawejje, R., Krispin, R., & Covo, S. (2024) · Fungal Biology · doi:10.1016/j.funbio.2024.02.007 · Cited by 7 · see citing works
Structure-specific DNA endonuclease T7 endonuclease I cleaves DNA containing UV-induced DNA lesions
Matsubara, K., Ueda, S., Yamamoto, J., Iwai, S., Shioi, N., Takedachi, A., & Kuraoka, I. (2024) · The Journal of Biochemistry · doi:10.1093/jb/mvae024 · Cited by 3 · see citing works
Doetsch, P. W., Beljanski, V., & Song, B. (2005) · NSUWorks (Nova Southeastern University) · Cited by 4 · see citing works
Mitochondrial Genetics in a Petite-Negative Yeast
Schäfer, B., & WOLF, K. W. (2004) · Springer eBooks · doi:10.1007/978-3-662-10360-9_28 · Cited by 4 · see citing works
The Role of Mitochondrial Oxidative Stress in Retinal Dysfunction
Jarrett, S. G., Lewin, A. S., & Boulton, M. E. (2012) · Humana Press eBooks · doi:10.1007/978-1-61779-606-7_10 · Cited by 3 · see citing works
Szabó, J. E., Nyíri, K., Andrási, D., Matejka, J., Ozohanics, O., & Vértessy, B. G. (2021) · Scientific Reports · doi:10.1038/s41598-021-98790-3 · Cited by 2 · see citing works
The Ultraviolet Damage Endonuclease (UVDE) Protein and Alternative Excision Repair
Beljanski, V., Song, B., & Doetsch, P. W. (2005) · CRC Press eBooks · doi:10.1201/9780849352683.ch11 · Cited by 1 · see citing works
Disorders of mitochondrial DNA polymerase: a clinical, biochemical and structural study
Palin, E. (2013) · Työväentutkimus Vuosikirja
Role of Mitochondrial Dynamics and Autophagy in Removal of Helix-Distorting Mitochondrial DNA Damage
Bess, A. S. (2012) · DukeSpace (Duke University)
Sorbic acid resistance: the inoculum effect
Steels, H., James, S. A., Roberts, I. N., & Stratford, M. (2000) · Yeast · doi:10.1002/1097-0061(20000930)16:13 · Cited by 92 · see citing works
Repair of Mitochondrial DNA Damage
Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A., & Ellenberger, T. E. (2014) · ASM Press eBooks · doi:10.1128/9781555816704.ch13
Alternative Excision Repair of DNA
Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A., & Ellenberger, T. E. (2014) · ASM Press eBooks · doi:10.1128/9781555816704.ch11
Jati, O. M., Astuti, R. I., & Wahyudi, A. T. (2025) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2025.103882
Fungal DNA damage repair: from model to driver of virulence and AMR
Parkin, C., Bainbridge, A. G., & Rack, J. G. M. (2026) · Bioscience Reports · doi:10.1042/bsr20250315
- Authors
- Yasuhira, S., & Yasui, A.
- Affiliations
- Tohoku University (JP)
- Institute of Development, Aging and Cancer, Tohoku University (JP)
- Corresponding author
- Yasuhira, Shinji
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-04
- Volume
- 275
- Issue
- 16
- Pages
- 11824-11828
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2003502465
- Cited by
- 34
- References
- 42
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2014: 2 · 2013: 4 · 2012: 3
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- excision repair, nucleotide excision repair, cyclobutane pyrimidine dimers, Schizosaccharomyces pombe, mitochondrial genome, nuclear genome, protein localization, UV resistance, DNA damage tolerance
- MeSH terms
- Cell Nucleus, Deoxyribonuclease I, DNA Repair, DNA, Fungal, Fungal Proteins, Kinetics, Mitochondria, Schizosaccharomyces, Ultraviolet Rays
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
