The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
A novel recovery system for cultured cells using plasma‐treated polystyrene dishes grafted with poly(N‐isopropylacrylamide)
Abstract
Poly(N‐isopropyl acrylamide) (PIPA Am) demonstrated a fully expanded chain conformation below 32°C and a collapsed, compact conformation at high temperatures. This unique temperature responsive polymer was grafted onto surfaces of commercial polystyrene dishes and used as temperature switches for creating hydrophilic surfaces below 32°C and hydrophobic surfaces above 32°C. Cells attachment and the growth of bovine endothelial cells and rat hepatocytes on PIPA Am‐grafted surfaces at 37°C demonstrated similar behavior to the commercialized culture dishes. Both cell types were observed to detach from the PIPA Am‐grafted surface simply by reducing the temperature below the polymer transition temperature (collapse). Cells recovered by this method maintained substrate adhesivity, growth, and secretion activities nearly identical to those found in primary cultured cells in contrast to the compromised function found in cultured cells damaged by trypsinization. These results provide strong evidence that PIPA Am‐grafted surfaces, as thermal switches are very effective for reversing cell attachment and detachment without cell damage. Properties of cell culture surfaces can be readily transformed by this technique reversibly into hydrophilic and hydrophobic coatings of PIPA Am‐grafted polymers. © 1993 John Wiley & Sons, Inc.
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Cites
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Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells
Yamada, N., Okano, T., Sakai, H., Karikusa, F., Sawasaki, Y., & Sakurai, Y. (1990) · Die Makromolekulare Chemie, Rapid Communications · doi:10.1002/marc.1990.030111109 · Cited by 983
Self-citationCellular Adhesiveness and Extracellular Substrata
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Thermally on-off switching polymers for drug permeation and release
Okano, T., Bae, Y. H., Jacobs, H., & Kim, S. W. (1990) · Journal of Controlled Release · doi:10.1016/0168-3659(90)90138-j · Cited by 616
Self-citationTemperature dependence of swelling of crosslinked poly(N,N′‐alkyl substituted acrylamides) in water
Bae, Y. H., Okano, T., & Kim, S. W. (1990) · Journal of Polymer Science Part B: Polymer Physics · doi:10.1002/polb.1990.090280609 · Cited by 578
Self-citationThermo‐sensitive polymers as on‐off switches for drug release
Bae, Y. H., Okano, T., Hsu, R., & Kim, S. W. (1987) · Die Makromolekulare Chemie, Rapid Communications · doi:10.1002/marc.1987.030081002 · Cited by 545
Self-citationBen-Ze'ev, A., Robinson, G. S., Bucher, N. L., & Farmer, S. R. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.7.2161 · Cited by 529
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Hydrogels in pharmaceutical formulations
Peppas, N. (2000) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/s0939-6411(00)00090-4 · Cited by 4,011
Scaffolding in tissue engineering: general approaches and tissue-specific considerations
Chan, B. P., & Leong, K. W. (2008) · European Spine Journal · doi:10.1007/s00586-008-0745-3 · Cited by 1,708
Thermoresponsive hydrogels in biomedical applications
Klouda, L., & Mikos, A. G. (2008) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2007.02.025 · Cited by 1,349
Chung, J. E., Yokoyama, M., Yamato, M., Aoyagi, T., Sakurai, Y., & Okano, T. (1999) · Journal of Controlled Release · doi:10.1016/s0168-3659(99)00029-2 · Cited by 599
Self-citationThermo-responsive polymers and their application as smart biomaterials
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Metadata
- Authors
- Okano, T., Yamada, N., Sakai, H., & Sakurai, Y.
- Venue
- Journal of Biomedical Materials Research
- Publisher
- Wiley
- Published
- 1993-10
- Volume
- 27
- Issue
- 10
- Pages
- 1243-1251
- ISSN
- 0021-9304 (ISSN-L)
- 1097-4636
- OpenAlex
- W1980780439
- Cited by
- 1,020
- References
- 18
- FWCI
- 5.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- Okano, T., Yamada, N., Sakai, H., & Sakurai, Y. (1993). A novel recovery system for cultured cells using plasma‐treated polystyrene dishes grafted with poly(N‐isopropylacrylamide). Journal of Biomedical Materials Research. https://doi.org/10.1002/jbm.820271005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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